Dietary probiotic-derived extracellular vesicles as delivery systems of bioactive compounds to maintain intestinal homeostasis

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159546 - 159546

Published: Jan. 1, 2025

Language: Английский

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Odoribacter splanchnicus-derived extracellular vesicles alleviate inflammatory bowel disease by modulating gastrointestinal inflammation and intestinal barrier function via the NLRP3 inflammasome suppression

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: Feb. 11, 2025

Abstract Background Extracellular vesicles (EVs) derived from specific bacteria exert therapeutic potential on inflammatory diseases. Previous reports suggest the protective role of Odoribacter splanchnicus ( O.splanchnicus ) in bowel disease (IBD). The effect EVs (Os-EVs) and underlying mechanism IBD were surveyed here. Methods Os-EVs with ultracentrifugation before characterization by transmission electron microscopy nanoparticle tracking analysis. Based model mice induced dextran sulfate sodium (DSS), effects symptoms, intestinal barrier dysfunction, colonic apoptosis, inflammation as well NLRP3 inflammasome activation analyzed. knockout exploited to judge against IBD. Results typically shaped a double concave disc (average diameter = 95 nm). administration attenuated DSS-induced body weight loss, colon shortening, activity index score, histological injury mice. could also relieve dysfunction evidenced up-regulation zona occludens-1 Occludin decrease TUNEL-positive staining tissues downregulated expression interleukin-1β (IL-1β), tumor necrosis factor-α, IL-6, elevated IL-10, accompanied blockage Furthermore, experiments revealed that relies regulating NLRP3. Conclusion Our finding indicated effectively ameliorated through repressing NLRP3, strongly supporting probiotic-derived for alleviating

Language: Английский

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Lactic acid bacteria derived extracellular vesicles: emerging bioactive nanoparticles in modulating host health

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Nov. 13, 2024

Lactic acid bacteria derived extracellular vesicles (LAB-EVs) are nano-sized and carry a variety of biological cargoes. LAB-EVs have proven to be potential mediators intercellular communication, serving not only the parental but also host cell in both physiology pathology. therapeutically beneficial various diseases through cell-free strategy. Particularly, EVs secreted from probiotics can exert health-promoting effects on humans. Additionally, excitement around has extended their use as drug carriers, since they traverse barriers. Nevertheless, significant challenges terms isolation, characterization, safety must addressed ensure clinical application LAB-EVs. Therefore, this review emphasizes isolation purification methods We introduce biogenesis, cargo sorting, functions The regulatory factors summarized discussed. Special attention is given interaction between host, ability maintain intestinal homeostasis, immunity inflammation induce diverse diseases. Furthermore, we summarize characterization LAB-EV cargoes by advanced analytical such proteomics. Finally, discuss opportunities means diagnosis treatment translation. In conclusion, scrutinizes current knowledge provides guidelines for proposing new perspectives future research field

Language: Английский

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Impact of probiotics-derived extracellular vesicles on livestock gut barrier function

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 7, 2024

Abstract Probiotic extracellular vesicles (pEVs) are biologically active nanoparticle structures that can regulate the intestinal tract through direct or indirect mechanisms. They enhance barrier function in livestock and poultry help alleviate diseases. The specific effects of pEVs depend on their internal functional components, including nucleic acids, proteins, lipids, other substances. This paper presents a narrative review impact across various segments tract, exploring mechanisms action while highlighting limitations current research. Investigating which probiotics operate via could deepen our understanding provide theoretical foundation for application production.

Language: Английский

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Limosilactobacillus reuteri ZY15 Alleviates Intestinal Inflammation and Barrier Dysfunction via AKT/mTOR/HIF-1α/RORγt/IL-17 Signaling and the Gut Microbiota in ETEC K88-Challenged Mice

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 58 - 58

Published: Jan. 6, 2025

Limosilactobacillus reuteri, a recognized probiotic, improves intestinal health in animals, but the mechanism remains unclear. This study investigates mechanisms by which L. reuteri ZY15, isolated from healthy pig feces, mitigates barrier damage and inflammation caused oxidative stress Enterotoxigenic Escherichia coli (ETEC) K88-challenged mice. The results indicated that ZY15 increased antioxidant capacity reducing serum reactive oxygen species (ROS) superoxide dismutase (SOD) levels. enhanced upregulating mucin 1, 2, occludin, zonula occludens-1 (ZO-1), claudin-1 expressions protein mRNA It significantly alleviated proinflammatory cytokines interleukin-1β (IL-1β), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-17 (IL-17) Notably, suppressed inhibiting AKT/mTOR/HIF-1α/RORγt/IL-17 pathway activation. Additionally, it altered structure of gut microorganisms enriching Akkermansia Clostridia_UCG.014, thereby re-establishing colonization resistance alleviating ETEC K88-induced Taken together, our findings reveal protective mice challenged with K88 regulating signaling microbial imbalance. Leveraging these properties, live offers promising alternative treatment for coli-induced diarrhea weaned piglets.

Language: Английский

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Serum metabolomic analysis in mice exposed to perfluorooctanoic acid and sodium p-perfluorous nonenoxybenzene sulfonate

Molecular & Cellular Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Language: Английский

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Seleno-chitooligosaccharide-induced modulation of intestinal barrier function: Role of inflammatory cytokines, tight junction proteins, and gut microbiota in mice

Journal of Applied Biomedicine, Journal Year: 2025, Volume and Issue: 23(1), P. 45 - 55

Published: March 27, 2025

This study aimed to explore the function of Seleno-chitooligosaccharide (SOA) on intestinal barrier through regulation inflammatory cytokines, tight junction protein, and gut microbiota in mice. The results ELISA assay demonstrated that SOA significantly increased levels IL-2, IL-10, IFN-γ serum ileum. Meanwhile, IL-4 ileum (p < 0.05). In addition, Diamine Oxidase (DAO) concentration was decreased by treatments administration upregulated expression ZO-1 Occludin By 16S rDNA sequencing, reduced ratio Bacillota/Bacteroidota observed treated Within phylum Bacteroidota, relative abundance Deferribacterota, uncultured Bacteroidales bacterium, Bacteroides. Bacillota, Erysipelatoclostridium Lachnoclostridium, Ruminococcaceae UCG-010 were with supplement. summary, has potential modulate prevent diseases.

Language: Английский

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Chlorogenic acid inhibits virulence and resistance gene transfer in outer membrane vesicles of carbapenem-resistant Klebsiella pneumoniae

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 31, 2025

Carbapenem-resistant Klebsiella pneumoniae (CRKp) infection poses a significant global public health challenge, with the misuse of antibiotics further contributing to development resistance and triggering harmful inflammatory responses. Outer membrane vesicles (OMVs) released by CRKp under sub-lethal concentration MEM pressure (KOMV-MEM) exhibit enhanced virulence greater efficiency in transferring genes. We investigated inhibitory effects chlorogenic acid (CA) on KOMV-MEM characteristics its protective role infected mice. Based LC-MS proteomic analysis vesicles, we screened for potential targets promoting macrophage (MØ) pyroptosis pathways inducing gene transfer. Subsequently, computational predictions experimental validation were performed determine how CA regulates these mechanisms. This study confirmed that, pressure, exacerbated levels CRKp-inoculated mice are attributable high KOMV-MEM. Computational results demonstrated that inhibits reducing MØ capture through blocking interaction between GroEL LOX-1. Furthermore, prevents spread genes disrupting conjugation transfer processes recipient bacteria. Finally, vitro vivo assays showed enzymes, thereby preventing hydrolysis environment depriving susceptible bacteria protection. These findings provide first confirmation can inhibit both transmission drug underscores treatment as promising antimicrobial strategy against infection.

Language: Английский

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Faecalibacterium prausnitzii-derived outer membrane vesicles reprogram gut microbiota metabolism to alleviate Porcine Epidemic Diarrhea Virus infection

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 2, 2025

The Porcine Epidemic Diarrhea Virus (PEDV) is one of the major challenges facing global pig farming industry, and vaccines treatments have proven difficult in controlling its spread. Faecalibacterium prausnitzii (F.prausnitzii), a key commensal bacterium gut, has been recognized as promising candidate for next-generation probiotics due to potential wide-ranging health benefits. A decrease F.prausnitzii abundance associated with certain viral infections, suggesting application preventing intestinal infections. In this study, we utilized piglet model examine role PEDV infection was established supplemented F.prausnitzii, revealing that mitigated infection. Further studies found outer membrane vesicles (OMVs) are main functional components proteomics, untargeted metabolomics, small RNA-seq were used analyze composition OMVs. Exhaustion gut microbiota demonstrated function Fp. OMVs relies on presence microbiota. Additionally, metagenomic analysis indicated altered composition, enhancing prausnitzii, Prevotellamassilia timonensis, Limosilactobacillus reuteri. Untargeted metabolomics showed increased phosphatidylcholine (PC) levels, PC identified metabolite alleviating Single-cell sequencing revealed relative cells, number epithelial reduced necroptosis target cells. treatment infected IPEC-J2 Vero cells alleviated activation RIPK1-RIPK3-MLKL signaling axis, thereby improving play critical mitigating These findings provide strategy ameliorate piglets. Video Abstract.

Language: Английский

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Assessment of Antimicrobial Potential of Lactiplantibacillus plantarum and Their Derived Extracellular Vesicles

Molecular Nutrition & Food Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

ABSTRACT Scope Probiotic‐derived antimicrobial substances offer a promising treatment approach with less resistance risk. This study evaluated the activity of 20 Lactiplantibacillus plantarum strains human gut origin and their derived extracellular vesicles (EVs) against pathogens Staphylococcus aureus MTCC 96 Propionibacterium acnes 1950. Methods results The cell‐free supernatants (CFSs) all Lactobacillus isolates were initially screened for potential selected pathogens. CFSs further concentrated subjected to SDS PAGE, bands excised analysis activity. Additionally, EVs isolated characterized from LpJ5 LpJ15 Results indicated that out isolates, CFS (pH 6.5) both demonstrated highest zones inhibition S. (22.9 ± 1.00 mm) P. (15.66 1.52 mm), respectively. showed 18.5 0.2 mm 0.3 . Conclusion Findings this highlight two potent LpJ15, exhibit substantial activity, suggest application as biotherapeutic agents

Language: Английский

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