Cancer Reports, Journal Year: 2024, Volume and Issue: 7(8)
Published: Aug. 1, 2024
Integrin-Binding Sialoprotein (IBSP) has been implicated in tumor progression across various cancers. However, the specific role of IBSP breast cancer remains underexplored. There is a need to investigate mechanisms by which influences and its potential as therapeutic target.
Language: Английский
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137708 - 137708
Published: Nov. 1, 2024
Language: Английский
Citations
5
European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)
Published: Feb. 8, 2025
Emerging evidences have indicated a role of the complement system in pathogenesis diabetic nephropathy (DN). Thus, this study was conducted to explore system-related key biomarkers for patients with DN. DN microarray datasets were downloaded from GEO database, followed by differentially expressed genes (DEGs) screening. Complement (CSRGs) searched various databases. Weighted Gene Co-expression Network Analysis (WGCNA) employed screen DN-related genes, then differential CSRGs (DCSRGs) identified, protein–protein interaction (PPI) network construction. In addition, acquired two machine learning algorithms, immune infiltration analysis, Set Enrichment (GSEA), and potential drugs screening conducted. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) western blotting utilized detect ITGB2 expression. Then cell viability, inflammatory factors, expression epithelial–mesenchymal transition (EMT) fibrosis markers determined using Cell Counting Kit-8 (CCK-8) assay, enzyme linked immunosorbent assay (ELISA), assays, respectively. total, 1012 DEGs 974 screened, intersection analysis three (DN-related CSRGs) yielded 13 which considered as DCSRGs. Subsequently, 2 identified learning, namely VWF ITGB2. The both enriched pathways chemokine signaling pathway, CAMs, focal adhesion natural killer cell-mediated cytotoxicity, significantly correlated activated mast cells, resting NK macrophages. Also, related clinical features, including age, serum creatinine level, GFR (MDRD). Besides, mRNA protein levels HG-treated HK-2 cells remarkably elevated. Moreover, viability TNF-α, IL-6, IL-12, α-SMA, E-cadherin vimentin changed HG administration reversed ITGB2-silence. gene overexpressed DN, inhibition attenuated EMT inflammation
Language: Английский
Citations
0
Starch - Stärke, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 10, 2025
ABSTRACT The biocompatible and biodegradable polysaccharide starch is a great platform for the development of nanoparticles (NPs). Due to its special qualities, which include high stability, low toxicity, ease modification, it desirable option administration anticancer drugs. Targeted cancer therapy has found potential carrier in starch‐based (SNPs). SNPs can be functionalized with ligands target cells particular. These allow targeted medication delivery by identifying overexpressed receptors on tumor surfaces. have been studied relation chemotherapeutics, siRNAs, photothermal treatments, among other agents. They are also used combination therapy, where their combined targeting improves treatment outcomes. being evaluated number clinical trials. trials evaluate patient outcomes, safety, efficacy offer important information future uses. Regulatory bodies closely monitor various treatments based NPs. Clinical translation requires an understanding safety profiles, biodistribution, clearance mechanisms. In this review, we examine enhance through drug targeting.
Language: Английский
Citations
0
European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)
Published: Feb. 12, 2025
Dysregulation of long non-coding RNA H19 (lncRNA H19) is involved in cervical cancer (CC) progression. This study aims to unveil the specific role and relevant mechanism lncRNA CC. The expression CC cells was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). were transfected with sh-H19, followed cell proliferation, apoptosis, migration invasion examined. After location using fluorescence Situ Hybridization (FISH), target microRNAs (miRNAs) genes associated predicted bioinformatics analysis validated dual-luciferase reporter assay. Finally, explored vivo. upregulation observed cells. LncRNA knockdown inhibited migration, cells, remarkably promoted apoptosis. localized nucleus interacted miR-140 that downregulated MiR-140 inhibition reversed effects on development. targets ALDH1A1, decreased ALDH1A1 expression, which rescued inhibition. In vivo experiments also shown reduction diminishes tumor growth via targeting miR-140/ALDH1A1 axis. promotes malignant progression through
Language: Английский
Citations
0
European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)
Published: Feb. 19, 2025
To investigate the influence of MEK5/ERK5 pathway on mitophagy in osteosarcoma (OS), as well involved molecular mechanisms. The overlapped genes mitophagy-related from MSigDB database and DEGs between metastatic primary OS groups GSE32981 were identified. GSVA pathways analyzed. relationships Nur77 pathways, prognosis, immune infiltrating cells, response gene sets investigated. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) western blotting utilized to assess expression levels MEK5, ERK5, Nur77, PINK1, Parkin. Cellular behaviors mitochondrial potential evaluated via CCK-8, Transwell assay JC-1 staining. Total 4 obtained DEGs, including GABARAPL1, HIF1A, RB1CC1. activity scores 3 exhibited significant differences groups. Importantly, was significantly negatively correlated with a (GOBP MITOPHAGY: R = − 0.48, P 0.02). group remarkedly higher than that (P < 0.001). Patients high had poor AUC values all above 0.615 predicting 1-, 3-, 5-year survival. In addition, closely related numerous activated dendritic mast cells M0 macrophages, chemokines cytokines (all 0.05). is OS, overexpressed MEK5/ERK promotes expression, tumorigenesis function U2OS cells. Cytosporone B implement increased sh-MEK5 group, inhibited weaken membrane caused by MEK5 downregulation, reversed protein markers PINK1 Parkin group. MEK5–ERK5 mediates regulating promote These findings offered promising therapeutic targets for OS.
Language: Английский
Citations
0
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: March 4, 2025
The tumor microenvironment (TME) is a complex and dynamic ecosystem that plays critical role in cancer progression. It comprises various cell types, including immune cells, stromal cells. Among these, cancer-associated fibroblasts (CAFs) represent heterogeneous population with diverse origins, phenotypes, functions. Activated CAFs secrete multiple factors promote growth, migration, angiogenesis, contribute to chemoresistance. Additionally, extracellular matrix (ECM) components, such as collagen, which form physical barrier hinders the penetration of chemotherapeutic immunotherapeutic agents. This ECM also influences infiltration, impeding their ability effectively target As result, modulating activity has emerged promising strategy enhance efficacy immunotherapy. Nano-delivery systems, constructed from nanomaterials high targeting specificity biocompatibility, offer compelling approach deliver therapeutic agents or immunomodulatory directly CAFs. modulation can alter CAF function, reduce tumor-promoting effects, thereby improve outcomes review provides an in-depth exploration functions, interactions within TME, particularly context suppression. Furthermore, it discusses potential applications functional nanocarrifers enhancing effectiveness immunotherapy, highlighting significant progress nanotechnology this area.
Language: Английский
Citations
0
Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101203 - 101203
Published: Jan. 1, 2025
Language: Английский
Citations
0
Cancer Reports, Journal Year: 2024, Volume and Issue: 7(8)
Published: Aug. 1, 2024
Integrin-Binding Sialoprotein (IBSP) has been implicated in tumor progression across various cancers. However, the specific role of IBSP breast cancer remains underexplored. There is a need to investigate mechanisms by which influences and its potential as therapeutic target.
Language: Английский
Citations
2