Cell-type-specific expression of Nav1.6 in the developing brain of mice and its involvement in glial activation in Alzheimer’s disease DOI
Khabir Ahmad, Bakhtawar Khan, Khizar Khan

et al.

Cerebral Cortex, Journal Year: 2025, Volume and Issue: 35(2)

Published: Feb. 1, 2025

Voltage-gated sodium channels (Nav's), particularly Nav1.6, are crucial for action potential generation in neurons and linked to brain disorders. This study explores the cell-type-specific expression of Nav1.6 C57BL/6 mice brains at various developmental stages. Coronal sections from embryonic day 14 postnatal 30 were examined. increased both protein messenger RNA (mRNA) levels during this period. Immunofluorescence double staining revealed that is primarily localized on neurons. Astrocytes show increasing prenatal stages, correlating with maturation. Microglia exhibit low-intensity throughout development. Co-expression oligodendrocyte precursor cell marker NG2 observed E14 through prominent co-expression 21 30. Consistent olig2 E16 In primary cultures, astrocytes had higher compared microglia cells. was upregulated APP/amyloid beta protein/presenilin 1 (PS1) transgenic mice. Down-regulating vitro reduced amyloid beta-induced microglial activation cytokine (IL-1β, TNF-α). These findings highlight as a target therapeutic interventions against neurodegenerative diseases.

Language: Английский

Cell-type-specific expression of Nav1.6 in the developing brain of mice and its involvement in glial activation in Alzheimer’s disease DOI
Khabir Ahmad, Bakhtawar Khan, Khizar Khan

et al.

Cerebral Cortex, Journal Year: 2025, Volume and Issue: 35(2)

Published: Feb. 1, 2025

Voltage-gated sodium channels (Nav's), particularly Nav1.6, are crucial for action potential generation in neurons and linked to brain disorders. This study explores the cell-type-specific expression of Nav1.6 C57BL/6 mice brains at various developmental stages. Coronal sections from embryonic day 14 postnatal 30 were examined. increased both protein messenger RNA (mRNA) levels during this period. Immunofluorescence double staining revealed that is primarily localized on neurons. Astrocytes show increasing prenatal stages, correlating with maturation. Microglia exhibit low-intensity throughout development. Co-expression oligodendrocyte precursor cell marker NG2 observed E14 through prominent co-expression 21 30. Consistent olig2 E16 In primary cultures, astrocytes had higher compared microglia cells. was upregulated APP/amyloid beta protein/presenilin 1 (PS1) transgenic mice. Down-regulating vitro reduced amyloid beta-induced microglial activation cytokine (IL-1β, TNF-α). These findings highlight as a target therapeutic interventions against neurodegenerative diseases.

Language: Английский

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