Promoting activities of human cyclophilin A on calcium oxalate stone formation at crystal growth, aggregation and crystal-cell adhesion phases DOI Creative Commons

Sudarat Hadpech,

Waralee Srinarawat,

Visith Thongboonkerd

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143374 - 143374

Published: April 1, 2025

Kidney stone is a specialized form of biomineralization involving complex interactions between crystals and urinary macromolecules. Renal tubular cells secrete cyclophilin A (CyPA), protein whose elevated level associated with various kidney diseases. Nevertheless, its role in formation has not previously been explored. This study thus aimed to investigate roles CyPA through calcium oxalate (CaOx) crystal assays. Recombinant human was generated mimic secretory excreted into the urine. Crystal assays yielded first evidence demonstrating that significantly promoted CaOx growth, aggregation crystal-cell adhesion, all which are critical steps during initial formation. Despite lack specific Ca2+-binding Ox2--binding domains inability bind free Ca2+/Ox2- ions solution, demonstrated distinct ability crystals. Upon binding, facilitated further adjacent adhesion. These findings unravel novel mechanism pathogenesis, expanding known functions CyPA. research also provides solid how became one compositions matrix highlights potential be therapeutic/preventive target for management/prevention disease.

Language: Английский

Promoting activities of human cyclophilin A on calcium oxalate stone formation at crystal growth, aggregation and crystal-cell adhesion phases DOI Creative Commons

Sudarat Hadpech,

Waralee Srinarawat,

Visith Thongboonkerd

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143374 - 143374

Published: April 1, 2025

Kidney stone is a specialized form of biomineralization involving complex interactions between crystals and urinary macromolecules. Renal tubular cells secrete cyclophilin A (CyPA), protein whose elevated level associated with various kidney diseases. Nevertheless, its role in formation has not previously been explored. This study thus aimed to investigate roles CyPA through calcium oxalate (CaOx) crystal assays. Recombinant human was generated mimic secretory excreted into the urine. Crystal assays yielded first evidence demonstrating that significantly promoted CaOx growth, aggregation crystal-cell adhesion, all which are critical steps during initial formation. Despite lack specific Ca2+-binding Ox2--binding domains inability bind free Ca2+/Ox2- ions solution, demonstrated distinct ability crystals. Upon binding, facilitated further adjacent adhesion. These findings unravel novel mechanism pathogenesis, expanding known functions CyPA. research also provides solid how became one compositions matrix highlights potential be therapeutic/preventive target for management/prevention disease.

Language: Английский

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