Frontiers in Cell and Developmental Biology,
Journal Year:
2022,
Volume and Issue:
10
Published: April 20, 2022
Considerable
progress
has
been
made
in
our
knowledge
of
the
morphological
and
functional
varieties
anchoring
junctions.
Cell-cell
adhesion
contacts
consist
discrete
junctional
structures
responsible
for
mechanical
coupling
cytoskeletons
allow
transmission
signals
across
cell
collective.
The
three
main
complexes
are
adherens
junctions,
tight
desmosomes.
Microscopy
played
a
fundamental
role
understanding
these
on
different
levels
both
physiological
pathological
conditions.
In
this
review,
we
discuss
light
electron
microscopy
techniques
used
to
unravel
structure
composition
cell-cell
epithelial
endothelial
cells.
It
functions
as
guide
pick
appropriate
imaging
technique(s)
interest.
We
also
point
out
latest
that
have
emerged.
At
end,
problems
investigators
encounter
during
their
research
using
microscopic
techniques.
The Journal of Physical Chemistry B,
Journal Year:
2023,
Volume and Issue:
127(21), P. 4708 - 4721
Published: May 19, 2023
Recent
advances
in
fluorescence-based
microscopy
techniques,
such
as
single
molecule
fluorescence,
Förster
resonance
energy
transfer
(FRET),
fluorescence
intensity
fluctuations
analysis,
and
super-resolution
have
expanded
our
ability
to
study
proteins
greater
detail
within
their
native
cellular
environment
investigate
the
roles
that
protein
interactions
play
biological
functions,
inter-
intracellular
signaling
cargo
transport.
In
this
Perspective,
we
provide
an
up-to-date
overview
of
current
state
art
detection
living
cells
with
emphasis
on
recent
developments
facilitated
characterization
spatial
temporal
organization
into
oligomeric
complexes
presence
absence
natural
artificial
ligands.
Further
advancements
field
will
only
deepen
understanding
underlying
mechanisms
processes
help
develop
new
therapeutic
targets.
Optica,
Journal Year:
2023,
Volume and Issue:
10(9), P. 1147 - 1147
Published: July 31, 2023
The
need
to
observe
objects
that
are
smaller
than
the
diffraction
limit
has
led
development
of
various
superresolution
techniques.
However,
most
such
techniques
require
active
interaction
with
sample,
which
may
not
be
possible
in
multiple
practical
scenarios.
recently
developed
technique
Hermite–Gaussian
imaging
(HGI)
achieves
by
passively
observing
light
coming
from
an
object.
This
approach
involves
decomposing
incoming
field
into
basis
spatial
modes
and
measuring
amplitude
or
intensity
each
component.
From
these
measurements,
original
object
can
reconstructed.
implementing
HGI
experimentally
proven
challenging,
previous
achievements
have
focused
on
coherent
parameter
estimation
simple
objects.
In
this
paper,
we
implement
interferometric
incoherent
regime
demonstrate
a
three-fold
improvement
resolution
compared
direct
imaging.
We
evaluate
performance
our
method
under
different
noise
levels.
Our
results
constitute
step
towards
powerful
passive
fluorescent
microscopy
astronomy.
Lab on a Chip,
Journal Year:
2023,
Volume and Issue:
23(5), P. 1011 - 1033
Published: Jan. 1, 2023
We
discuss
the
recent
trends
in
integrating
deep-learning
(DL)
and
optofluidic
imaging.
A
holistic
understanding
of
them
could
incentivize
DL-powered
imaging
for
advancing
a
wide
range
novel
applications
science
biomedicine.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Feb. 24, 2024
Abstract
Recent
work
by
our
laboratory
and
others
indicates
that
co-display
of
multiple
antigens
on
protein-based
nanoparticles
may
be
key
to
induce
cross-reactive
antibodies
provide
broad
protection
against
disease.
To
reach
the
ultimate
goal
a
universal
vaccine
for
seasonal
influenza,
mosaic
influenza
nanoparticle
(FluMos-v1)
was
developed
clinical
trial
(NCT04896086).
FluMos-v1
is
unique
in
it
designed
four
recently
circulating
haemagglutinin
(HA)
strains;
however,
current
analysis
techniques
are
limited
population
analysis,
thus,
unable
determine
valency
an
individual
nanoparticle.
For
first
time,
we
demonstrate
total
internal
reflection
fluorescence
microscopy
supportive
physical–chemical
methods
indeed
achieved
single
nanoparticles.
Additionally,
have
determined
percentages
multivalent
(mosaic)
with
four,
three,
or
two
HA
proteins.
The
integrated
imaging
physicochemical
multivalency
will
serve
further
understand
immunogenicity
data
from
trial.
Frontiers in Cell and Developmental Biology,
Journal Year:
2022,
Volume and Issue:
10
Published: April 20, 2022
Considerable
progress
has
been
made
in
our
knowledge
of
the
morphological
and
functional
varieties
anchoring
junctions.
Cell-cell
adhesion
contacts
consist
discrete
junctional
structures
responsible
for
mechanical
coupling
cytoskeletons
allow
transmission
signals
across
cell
collective.
The
three
main
complexes
are
adherens
junctions,
tight
desmosomes.
Microscopy
played
a
fundamental
role
understanding
these
on
different
levels
both
physiological
pathological
conditions.
In
this
review,
we
discuss
light
electron
microscopy
techniques
used
to
unravel
structure
composition
cell-cell
epithelial
endothelial
cells.
It
functions
as
guide
pick
appropriate
imaging
technique(s)
interest.
We
also
point
out
latest
that
have
emerged.
At
end,
problems
investigators
encounter
during
their
research
using
microscopic
techniques.
