Recommendations for Design, Execution, and Reporting of Studies on Experimental Thoracic Aortopathy in Preclinical Models

Arteriosclerosis Thrombosis and Vascular Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

There is a recent dramatic increase in research on thoracic aortic diseases that includes aneurysms, dissections, and rupture. Experimental studies predominantly use mice which aortopathy induced by chemical interventions, genetic manipulations, or both. Many parameters should be deliberated experimental design concert with multiple considerations when providing dimensional data characterization of tissues. The purpose this review to provide recommendations guidance (1) the selection mouse model conditions for study, (2) standardizing detection measurements diseases, (3) meaningful interpretation characteristics diseased tissue, (4) reporting standards include rigor transparency.

Language: Английский

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KLF15 regulates macrophage polarization patterns in deep vein thrombosis

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 155, P. 114632 - 114632

Published: April 10, 2025

KLF15 is involved in cardiovascular disease processes by regulating vascular remodeling and metabolic disorders. Macrophages mediate the inflammatory response deep vein thrombosis (DVT) secreting cytokines modulating fibrinolytic system. Therefore, this study aims to discuss effect of on macrophage polarization DVT. In vivo, a DVT animal model was used assess expression polarization. vitro, PMA-treated THP-1 cells with overexpression were differentiated into M1- M2-like macrophages, markers analyzed molecular cellular assays. vivo experiments, levels KLF15, iNOS, CD206, IL-1β, IL-6, IL-10 TGF-β increased model. vitro augmented CD86, IL-12, TNF-α, IL-6 M1-like macrophages. Additionally, diminished IL-10, ARG1, CUT&Tag, peaks bound lgG mainly located promoter intronic regions, protein more than near TSS site. YY1, EIF4E, LCK, HMGB1, GPD2, MORF4L1, HIPK2 NEK2 hub genes that bind KLF15. ChIP assay confirmed macrophages enhanced its transcription NF-κB pathway activity. facilitates M1 via NEK2/NF-κB pathway, highlighting potential as therapeutic target for management. Future studies are warranted explore clinical applicability mechanistic nuances.

Language: Английский

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Identification of BTC and KLF15 in Vascular Smooth Muscle Cells as Key Biomarkers for Abdominal Aortic Aneurysm via Machine Learning and In Vitro Experiments

Cardiovascular Innovations and Applications, Journal Year: 2025, Volume and Issue: 10(1)

Published: Jan. 1, 2025

Objective: We aimed to identify molecular candidates serve as diagnostic biomarkers and therapeutic targets for abdominal aortic aneurysm (AAA). Method: conducted integrative bioinformatics analysis used machine learning key genetic screening. Gene expression profiles at the single-cell level were validated through in vitro experiments. Results: identified Betacellulin (BTC) Kruppel-like transcription factor 15 (KLF15) potential AAA. Notably, BTC is expressed predominantly vascular smooth muscle cells (VSMCs) AAA, whereas KLF15 VSMCs, fibroblasts, other cell types. RT-qPCR a significant decrease mRNA levels of VSMCs after angiotensin II administration. All-trans retinal, which interacts with BTC, was drug AAA treatment. RAD21 might be common TF driving both expression. Conclusions: two agent thus enhancing understanding mechanisms underlying disease offering novel strategies its clinical management.

Language: Английский

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KLF15 prevents ferroptosis in vascular smooth muscle cells via interacting with p53

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: 770, P. 152029 - 152029

Published: May 15, 2025

Language: Английский

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