Science Advances, Journal Year: 2025, Volume and Issue: 11(15)

Published: April 11, 2025

The discovery of histone H3 copper reductase activity provides a novel metabolic framework for understanding the functions core residues, which, unlike N-terminal have remained largely unexplored. We previously demonstrated that cysteine 110 (H3C110) contributes to cupric (Cu 2+ ) ion binding and its reduction cuprous 1+ form. However, this residue is absent in Saccharomyces cerevisiae , raising questions about evolutionary functional significance. Here, we report H3C110 has been lost many fungal lineages despite near-universal conservation across eukaryotes. Introduction into S. increased intracellular Cu levels ameliorated iron homeostasis defects caused by inactivation Cup1 metallothionein or glutathione depletion. Enhanced also extended replicative life span under oxidative growth conditions but reduced it fermentative conditions. Our findings suggest trade-off between activity, metabolism, may underlie loss retention

Language: Английский