Increased reproductive outcomes after optimized sperm preparation DOI Creative Commons
Matías D. Gómez‐Elías, Guillermina M. Luque,

Natalia Oscoz-Susino

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: May 13, 2025

A key factor to the success of in vitro fertilization (IVF) is preparation human sperm, a critical step that directly impacts efficacy procedure. This proof-of-concept study evaluated effect HyperSperm, novel sperm technique designed enhance function, on fertilization, embryo development, and pregnancy outcomes both mouse model first-in-human trial following IVF. In mice, HyperSperm treatment significantly increased hyperactivated motility (p < 0.05), leading improved blastocyst development as well higher implantation rates 0.05) larger litter sizes 0.05). Offspring displayed normal growth fertility. samples from normozoospermic men, exhibited high safety profile, with motility, acrosome reaction, viability, DNA fragmentation comparable controls. first-in-human, prospective, single-center, split-oocyte 10 couples undergoing IVF donated oocytes demonstrated similar between control groups = 0.425), but usable arm (43.8% vs. 67.9%, p 0.0122). Morphokinetic parameters were groups. These results suggest enhances hyperactivation, hallmark capacitation, mice humans. represents promising approach optimizing assisted reproduction, warranting further clinical investigation.

Language: Английский

Cytosolic and Acrosomal pH Regulation in Mammalian Sperm DOI Creative Commons
Julio C. Chávez,

Gabriela Carrasquel-Martínez,

Sandra Hernández-Garduño

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(10), P. 865 - 865

Published: May 17, 2024

As in most cells, intracellular pH regulation is fundamental for sperm physiology. Key functions like swimming, maturation, and a unique exocytotic process, the acrosome reaction, necessary gamete fusion, are deeply influenced by pH. Sperm regulation, both intracellularly within organelles such as acrosome, requires coordinated interplay of various transporters channels, ensuring that this cell primed fertilization. Consistent with pivotal importance mammalian physiology, several its dependent on cytosolic Examples include Ca

Language: Английский

Citations

4
Activation of motility and chemotaxis in the spermatozoa DOI Creative Commons
Manabu Yoshida, Kaoru Yoshida

Reproductive Medicine and Biology, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 1, 2025

Sperm motility and chemotaxis are important early steps in the interaction between sperm oocytes during fertilization. Understanding these processes is essential for their basic biological clinical applications. This review outlines advances understanding molecular mechanisms of activation over past two decades. The focuses on pathways Ca2+ signaling role CatSper channel involved this signaling, examines comprehensive that regulate aquatic invertebrates, fish, mammals. activated by environmental changes (e.g., pH osmolality) egg-derived factors. channels mediate influx cell chemotaxis. In addition to Ca2+, cAMP membrane potential also regulation motility. Alternative exist species lacking CatSper, highlighting diversity mechanisms. There has been significant progress mediated notably with but other factors remain unclear. Future research should focus uncover commonalities using genome editing transcriptomic analyses.

Language: Английский

Citations

0
Increased reproductive outcomes after optimized sperm preparation DOI Creative Commons
Matías D. Gómez‐Elías, Guillermina M. Luque,

Natalia Oscoz-Susino

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: May 13, 2025

A key factor to the success of in vitro fertilization (IVF) is preparation human sperm, a critical step that directly impacts efficacy procedure. This proof-of-concept study evaluated effect HyperSperm, novel sperm technique designed enhance function, on fertilization, embryo development, and pregnancy outcomes both mouse model first-in-human trial following IVF. In mice, HyperSperm treatment significantly increased hyperactivated motility (p < 0.05), leading improved blastocyst development as well higher implantation rates 0.05) larger litter sizes 0.05). Offspring displayed normal growth fertility. samples from normozoospermic men, exhibited high safety profile, with motility, acrosome reaction, viability, DNA fragmentation comparable controls. first-in-human, prospective, single-center, split-oocyte 10 couples undergoing IVF donated oocytes demonstrated similar between control groups = 0.425), but usable arm (43.8% vs. 67.9%, p 0.0122). Morphokinetic parameters were groups. These results suggest enhances hyperactivation, hallmark capacitation, mice humans. represents promising approach optimizing assisted reproduction, warranting further clinical investigation.

Language: Английский

Citations

0