Rational and Semirational Approaches for Engineering Salicylate Production in Escherichia coli DOI

Chenghu Chen,

Cong Gao, Guipeng Hu

et al.

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Salicylate plays a pivotal role as pharmaceutical intermediate in drugs, such aspirin and lamivudine. The low catalytic efficiency of key enzymes the inherent toxicity salicylates to cells pose significant challenges large-scale microbial production. In this study, we introduced salicylate synthase Irp9 into an l-phenylalanine-producing

Language: Английский

Biological effects of vanillic acid, iso-vanillic acid, and orto-vanillic acid as environmental pollutants DOI Creative Commons
Marzena Matejczyk, Piotr Ofman, Edyta Juszczuk‐Kubiak

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 277, P. 116383 - 116383

Published: April 24, 2024

Vanillic acid (4-hydroxy-3-methoxybenzoic acid) (VA) is a natural benzoic derivative commonly found in herbs, rice, maize, and some fruits vegetables. However, due to the wide use of VA various industrial sectors, its presence environment might harm living organisms. This study evaluated toxicity isomers, iso-VA orto-VA. Firstly, antimicrobial effect isomers orto-VA (in doses 1000; 100, 10, 1; 0.1; 0.01 mg/L) against Escherichia coli, Sarcina spp., Enterobacter homaechei, Staphylococcus aureus Candida albicans were identified. The toxic protein degradation potential determined using E. coli grpE:luxCDABE lac:luxCDABE biosensor strains. genotoxicity oxidative stress generation assessed with recA:luxCDABE strain. results showed that VA, iso-VA, exhibited activity all tested bacterial VA's differed from Similar toxic, genotoxic, stress-inducing effects observed for isomers. Each compound toxicity, cellular degradation, genotoxic grpE:luxCDABE, lac:luxCDABE, Analysis reactive oxygen species (ROS) within cells highlighted as contributing factor While findings suggest applications compounds food preservatives, their raises concerns regarding risks posed organisms characteristics.

Language: Английский

Citations

11
Emerging biotechnological strategies advancing biological lignin valorization towards polyhydroxyalkanoates DOI
Haiyuan Jia, Tao Xu,

Chen Wang

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132278 - 132278

Published: Feb. 1, 2025

Language: Английский

Citations

0
Mechanism and improvement of yeast tolerance to biomass-derived inhibitors: A review DOI

Jinling Yu,

Chaolun Li,

Y.W. Cheng

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108562 - 108562

Published: March 1, 2025

Language: Английский

Citations

0
Hydroxybenzoic acids: Microbial metabolism, pathway engineering and products DOI
Ingrida Kutraite, Ernesta Augustiniene, Naglis Malys

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108571 - 108571

Published: March 1, 2025

Language: Английский

Citations

0
Enhanced tolerance of Clostridium tyrobutyricum to lignin-derived phenolic acids by overexpressing native reductases DOI
Hailing Wei, Yuexin Wang, Yu Zeng

et al.

Journal of Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Exploring the Ligninolytic Capabilities of the Oleaginous Yeast Cutaneotrichosporon oleaginosum DOI
Bin Yang, Austin Gluth, Yunqiao Pu

et al.

Published: May 7, 2025

Abstract The current paradigm in synthetic biology for lignin bioconversion platforms includes primarily bacteria and filamentous fungi. Yeast are notoriously understudied their role degradation utilization, despite ubiquity saprophytic microbial communities. A few publications report ligninolytic yeasts, but investigations to date have relied on model aromatic compounds or lignin-containing substrates replete with other carbon sources. In this work, we use a suite of analytical tools evaluate interactions between corn stover-extracted the oleaginous yeast Cutaneotrichosporon oleaginosum. Notably, 2D-NMR analysis showed significant decrease H-lignin component as well resinol (β-β) phenylcoumaran (β-5) linkages. Using super-resolution fluorescence microscopy, demonstrated that may uptake polymeric lignin. To explore mechanisms degradation, transport, aromatics catabolism, extensive secretomics proteomics analyses were conducted. Compared carbon-limited glucose “No Carbon” controls, several putative laccases, quinone reductases, superoxide dismutases, glyoxal/oxalate oxidases upregulated condition. Excitingly, two ferric reductases oxalate exchanger only observed These results indicate C. oleaginosum perform extracellular redox cycling generate lignin-modifying reactive oxygen species. findings enhance our understanding utilization by provide valuable insights metabolic engineering.

Language: Английский

Citations

0
Assessment of lipid synthesis from sugarcane biomass by adaptive strains of Rhodosporidium toruloides DOI
Sâmilla Gabriella Coêlho de Almeida, Jonas Paulino de Souza, Henrique Maziero Fogarin

et al.

Biomass Conversion and Biorefinery, Journal Year: 2024, Volume and Issue: unknown

Published: April 2, 2024

Language: Английский

Citations

2
General mechanisms of weak acid-tolerance and current strategies for the development of tolerant yeasts DOI
Mengmeng Li,

Yunfei Chu,

Xiameng Dong

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 40(2)

Published: Dec. 22, 2023

Language: Английский

Citations

6
Adaptive evolution of Paecilomyces variotii enhanced the biodetoxification of high-titer inhibitors in pretreated lignocellulosic feedstock DOI

Agustian Chen,

Bin Zhang, Jie Bao

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 411, P. 131351 - 131351

Published: Aug. 24, 2024

Language: Английский

Citations

1
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates DOI
Linyue Tian,

Tay Ying Qi,

F. Zhang

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: unknown, P. 108477 - 108477

Published: Nov. 1, 2024

Language: Английский

Citations

1