Distinguishing the Metabolic Effect of Fetal and Adult Fibrinogen on Human Fibroblast Cell Culture by IR-MALDESI Mass Spectrometry Imaging DOI
Alena N. Joignant, Anastasia Sheridan, Ashley C. Brown

et al.

Journal of the American Society for Mass Spectrometry, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Mass spectrometry imaging (MSI) of cells can elucidate metabolic changes with cellular and molecular specificity. Fibroblasts are mesenchymal that important in tissue homeostasis wound healing. During early healing, fibroblasts adhere to fibrinogen migrate into fibrin clots, which interactions stabilize blood clots promote subsequent remodeling. It is understood exists distinct forms, fetal adult, have differing glycosylation morphological effects on fibroblasts. Despite their importance healing the extracellular environment, not commonly studied by MSI. While many MSI studies conducted at single-cell or subcellular level, there still utility accessing a broad view cell culture above spatial resolution. This enables wider area larger number directly from culture. In this work, dermal were imaged chamber slides infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI). method enabled treating chambers adult prior reduced sample preparation Many serum type elucidated, membrane lipids such as cholesterol ceramides potentially contributing observed types

Language: Английский

Distinguishing the Metabolic Effect of Fetal and Adult Fibrinogen on Human Fibroblast Cell Culture by IR-MALDESI Mass Spectrometry Imaging DOI
Alena N. Joignant, Anastasia Sheridan, Ashley C. Brown

et al.

Journal of the American Society for Mass Spectrometry, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Mass spectrometry imaging (MSI) of cells can elucidate metabolic changes with cellular and molecular specificity. Fibroblasts are mesenchymal that important in tissue homeostasis wound healing. During early healing, fibroblasts adhere to fibrinogen migrate into fibrin clots, which interactions stabilize blood clots promote subsequent remodeling. It is understood exists distinct forms, fetal adult, have differing glycosylation morphological effects on fibroblasts. Despite their importance healing the extracellular environment, not commonly studied by MSI. While many MSI studies conducted at single-cell or subcellular level, there still utility accessing a broad view cell culture above spatial resolution. This enables wider area larger number directly from culture. In this work, dermal were imaged chamber slides infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI). method enabled treating chambers adult prior reduced sample preparation Many serum type elucidated, membrane lipids such as cholesterol ceramides potentially contributing observed types

Language: Английский

Citations

0