Direct CO2 Transformation to Malate via Bioelectrosynthesis upon Engineered Shewanella oneidensis DOI
Yixin Li, Dong Xia, Yong Xie

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Microbial electrosynthesis (MES) offers a sustainable and low-carbon approach for CO2 valorization, with Shewanella oneidensis (S. oneidensis) MR-1 identified as an ideal microbe MES. However, no prior research has demonstrated that S. can directly metabolize into multicarbon (C2+) products due to its inability perform the intracellular formate assimilation pathway. Here, we provide initial proof-of-concept evidence of direct bioelectrochemical reduction C4 product malate. Specifically, transformation malate attains notable production concentration 1.18 mmol·L-1, marking first instance compound bioelectrosynthesis. Such remarkable CO2-to-C4 conversion performances are attributed successful implementation dual-plasmid systems in MR-1, which facilitate overexpression reductive glycine pathway (Plasmid I) assimilating CO2-derived alternative biosynthetic II) channel metabolic intermediates toward biosynthesis Advancing valorization carbon-negative C2+ bioproducts, our sophisticated engineered microbes be further refined scalable bioelectrolysis objective facilitating industrial applications.

Language: Английский

Direct CO2 Transformation to Malate via Bioelectrosynthesis upon Engineered Shewanella oneidensis DOI
Yixin Li, Dong Xia, Yong Xie

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Microbial electrosynthesis (MES) offers a sustainable and low-carbon approach for CO2 valorization, with Shewanella oneidensis (S. oneidensis) MR-1 identified as an ideal microbe MES. However, no prior research has demonstrated that S. can directly metabolize into multicarbon (C2+) products due to its inability perform the intracellular formate assimilation pathway. Here, we provide initial proof-of-concept evidence of direct bioelectrochemical reduction C4 product malate. Specifically, transformation malate attains notable production concentration 1.18 mmol·L-1, marking first instance compound bioelectrosynthesis. Such remarkable CO2-to-C4 conversion performances are attributed successful implementation dual-plasmid systems in MR-1, which facilitate overexpression reductive glycine pathway (Plasmid I) assimilating CO2-derived alternative biosynthetic II) channel metabolic intermediates toward biosynthesis Advancing valorization carbon-negative C2+ bioproducts, our sophisticated engineered microbes be further refined scalable bioelectrolysis objective facilitating industrial applications.

Language: Английский

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