A review of intact glycopeptide enrichment and glycan separation through hydrophilic interaction liquid chromatography stationary phase materials

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1735, P. 465318 - 465318

Published: Aug. 31, 2024

Language: Английский

Hydrophilic Sulfonate Covalent Organic Frameworks for Serum Glycopeptide Profiling

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1957 - 1957

Published: Feb. 24, 2025

Aberrant protein glycosylation is closely associated with a number of biological processes and diseases. However, characterizing the types post-translational modifications (PTMs) from complex samples challenging for comprehensive glycoproteomic analysis. The development high-performance enrichment materials strategies during sample pretreatment process prerequisite to glycoproteome research. Here in this work, sulfonate-rich covalent organic framework (COF) called TpPa-(SO3H)2 (referred as SCOF-2) was synthesized using Schiff base reaction identification glycopeptides. Benefiting high hydrophilicity abundant sulfonate affinity, total 28 16 glycopeptides could be efficiently detected standard glycoproteins horseradish peroxidase (HRP) immunoglobulin G (IgG) tryptic digest, respectively. Moreover, as-prepared SCOF-2 has an ultralow detection limit (0.01 fmol μL-1), excellent selectivity (molar ratio HRP:BSA = 1:5000), satisfactory recovery rate (89.1%), adsorption capacity (150 mg g-1) good reusability individual enrichment. Meanwhile, by adsorbent, 196 194 endogenous serum ovarian cancer patients healthy people among triplicates were successfully enriched identified, respectively, combined nanoLC-MS/MS technology. It demonstrated its great application potential glycoproteomics research provided novel insight design affinity materials.

Language: Английский

Advances in the development of N-glycopeptide enrichment materials based on hydrophilic interaction chromatography

Analytical and Bioanalytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Language: Английский