Bioanalysis, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 3
Published: Dec. 4, 2024
Language: Английский
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Jan. 6, 2025
Scorpion is a commonly used drug in traditional Chinese medicine for treating epilepsy, although the exact mechanisms are not yet fully understood. This study aimed to compare treatment effects of water extract (SWE) and ethanol (SEE) on mice with pentetrazole-induced epilepsy investigate possible through metabolomics methods. A epileptic model was assess corrective SWE SEE. Untargeted metabolomics, utilizing UPLC-Q-TOF/MS, employed analyze metabolic profiles identify changes following scorpion treatment. The results revealed that only showed therapeutic mice. Metabolomics analysis demonstrated significant alterations signatures between groups. By orthogonal partial least squares discrimination analysis, 44 108 potential biomarkers mouse serum were identified positive negative ion modes, respectively. Differential metabolites related then pinpoint relevant pathways mice, such as linoleic acid metabolism, biosynthesis unsaturated fatty acids, glycerophospholipid ether lipid metabolism. In conclusion, this highlights provides insight into underlying involved its efficacy.
Language: Английский
Citations
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Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 706 - 706
Published: Feb. 5, 2025
Plasma contains metabolites with diverse physicochemical properties, ranging from highly polar to apolar, and concentrations spanning at least nine orders of magnitude. metabolome analysis is valuable for monitoring health evaluating medical interventions but challenging due the metabolome’s diversity complexity. This study aims develop validate targeted LC-MS/MS methods quantifying 235 mammalian 17 compound classes in porcine plasma without prior derivatization. Utilizing reversed-phase hydrophilic interaction liquid chromatography coupled tandem mass spectrometry, each analyte identified quantified using two selected reaction (SRM) transitions. Fast polarity switching scheduled SRM enhance coverage throughput, enabling one sample about 40 min. A simple “dilute shoot” preparation protocol was employed, samples injected dilution levels align metabolite within calibration curve ranges. Validation included assessments carryover, linearity, detection quantification limits, repeatability recovery. The method further applied various animal species, demonstrating its applicability human studies. establishes robust comprehensive quantification, advancing large-scale metabolomics biomedical research.
Language: Английский
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Journal of Chromatography B, Journal Year: 2025, Volume and Issue: 1254, P. 124504 - 124504
Published: Feb. 5, 2025
Language: Английский
Citations
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Chemico-Biological Interactions, Journal Year: 2025, Volume and Issue: unknown, P. 111449 - 111449
Published: Feb. 1, 2025
Language: Английский
Citations
0
Food Safety and Health, Journal Year: 2025, Volume and Issue: unknown
Published: April 20, 2025
ABSTRACT Food safety is a critical public health concern for preventing foodborne illnesses and ensuring consumer protection. hazards may present throughout the food supply chain, from farm to fork, posing significant risks. This comprehensive review explored prevalent in Bangladesh highlighted smart sensor technologies hazard detection. By reviewing recent literature on Bangladeshi web, this study discusses potential consequences of these their detection methods. Finally, evaluation existing challenges sensor‐based techniques are provided. Bacterial pathogens, agrochemical residues, toxic preservatives, adulteration highly chain. The key country lack awareness, unhygienic practices handling preparation, multiplicity laws coordination among regulatory authorities, bureaucratic complexities, inadequate infrastructure skilled human resources. Smart offers promising solution limitations conventional determination techniques, providing rapid accurate results with low cost, portability, ease operation, thereby significantly enhancing country’s scenario. help policymakers, academicians better understand chain develop more effective strategies mitigating risks, safety, health.
Language: Английский
Citations
0
Published: Jan. 1, 2025
Language: Английский
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bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 7, 2024
Abstract High-performance liquid chromatography (HPLC) is a common medium-throughput technique to quantify the components of often complex mixtures like those typically obtained from biological tissue extracts. However, analysis HPLC data multianalyte samples hampered by lack tools accurately determine precise analyte quantities on level precision equivalent mass-spectrometry approaches. To address this problem, we developed tool call PeakClimber, that uses sum exponential Gaussian functions deconvolve overlapping, peaks in traces. Here analytically show are well-fit an function, PeakClimber more quantifies known peak areas than standard industry software for both and mass spectrometry applications, differences triglyceride abundances between colonized germ-free fruit flies.
Language: Английский
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Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(19)
Published: Oct. 1, 2024
Sinomenium acutum (Thunb.) Rehd. et Wils is widely used in the treatment of rheumatoid arthritis, with its alkaloid compound sinomenine (SIN) being renowned for significant anti-inflammatory properties. However, despite widespread application, vivo mechanisms and metabolic pathways SIN remain incompletely understood. This study established a rapid reliable method based on an ultra-high-performance liquid chromatography coupled Quadrupole-Exactive Orbitrap mass spectrometry molecular docking to identify characterize 69 metabolites rat plasma, urine, feces, revealing primary hydroxylation, demethylation, sulfation, glucuronidation. Molecular results revealed that phase I reactions, including dedimethylation, dehydrogenation, dihydroxylation, along their composite were pivotal influencing SIN's activity. M28, M36, M59 are potentially most active vivo. comprehensive analysis unveils pathways, offering insights into biological processes suggesting novel approach exploring drug constituents. These findings pave way further understanding mechanisms, contributing significantly development new therapeutic strategies.
Language: Английский
Citations
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Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: Nov. 6, 2024
Systemic lupus erythematosus (SLE) is a multifaceted autoimmune disease affecting various body organs and systems. The diagnosis of SLE its complications based on evident clinical symptoms, serological marker levels, pathological findings. Some markers have low sensitivity specificity, biopsy procedures are invasive in nature. Hence, metabolomics has emerged as valuable tool for screening categorization. Its application contributed significantly to identifying pathogenesis, improving diagnosis, developing treatment approaches. This review provides an overview the utilization study SLE, focusing advancements understanding disease’s aiding monitoring efficacy.
Language: Английский
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Bioanalysis, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 3
Published: Dec. 4, 2024
Language: Английский
Citations
0