A Photoelectrochemical Biosensor Mediated by CRISPR/Cas13a for Direct and Specific Detection of MiRNA-21 DOI Creative Commons
Yang Zhang, Pei Miao,

Jingyuan Wang

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(18), P. 6138 - 6138

Published: Sept. 23, 2024

Direct detection of miRNA is currently limited by the complex amplification and reverse transcription processes existing methods, leading to low sensitivity high operational demands. Herein, we developed a CRISPR/Cas13a-mediated photoelectrochemical (PEC) biosensing platform for direct sensitive miRNA-21. The specific recognition target miRNA-21 crRNA-21 eliminates need pre-amplification miRNA-21, thereby preventing signal distortion enhancing precision detection. When binds it activates trans-cleavage activity CRISPR/Cas13a, non-specific cleavage biotin-modified DNA with uracil bases (biotin-rU-DNA). This prevents biotin-rU-DNA from being immobilized on electrode surface. As result, streptavidin cannot attach via biotin binding, reducing spatial resistance causing positively correlated increase in photocurrent response. Cas-PEC biosensor has good analytical capabilities, linear responses between 10 fM nM, minimum limit 9 fM, an excellent recovery rate analysis real human serum samples. work presented innovative solution detecting other biomarkers bioanalysis clinical diagnostics.

Language: Английский

Near-Infrared-Responsive Photoelectrochemical Biosensors DOI
Kheibar Dashtian, Shaaker Hajati, Rezvan Karimi

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 179, P. 117890 - 117890

Published: July 30, 2024

Language: Английский

Citations

10
CRISPR detection of cardiac tumor-associated microRNAs DOI

Youlin Fu,

Peng Zhang, Feng Chen

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: Jan. 11, 2025

Language: Английский

Citations

1
Homogeneous Multicycle Cascaded DNA Circuit for Sensitive “Signal On-Off-Super On” PEC Biosensing DOI
Lingling Luo, Yuying Zhou, Yaqin Chai

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

The minor changes of miRNA levels due to various diseases and cancers bring great challenges for early diagnosis. Here we propose a "signal on-off-super on" PEC biosensor based on homogeneous multicycle cascaded DNA circuit SnSe/CdS photoanode sensitive detection biomarker miRNA-222. Specifically, Z-type heterojunction with greatly enhanced photoanodic performance was developed provide the initial "on" signal. target miRNA-222 converted dendritic structure through cascade circuit. signal can be switched off by further super loading photosensitizer manganese porphyrin (MnPP). It is worth noting that not only improved reaction kinetics but also avoided leakage Compared traditional "signal-on" or "signal-off" readout, this strategy avoids false response background, thereby enhancing sensitivity accuracy biosensor. limit constructed sensor 0.3 fM in linear range from 1 10 nM. outstanding reproducibility, stability, provides promising platform disease

Language: Английский

Citations

0
Lanthanide-doped nanoprobes for microRNA detection DOI Creative Commons
Yunya Liu, Yin Huang,

Yijun Luo

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 536, P. 216644 - 216644

Published: April 5, 2025

Language: Английский

Citations

0
A Photoelectrochemical Biosensor for miRNA Detection Utilizing SnS2-Modified Hollow In2O3 Nanotubes and Enzyme-Assisted DNA Walker Signal Amplification DOI

Le-Chuan Wang,

Yuping Wei, Jingshuai Chen

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 440, P. 137864 - 137864

Published: April 23, 2025

Language: Английский

Citations

0
A Photoelectrochemical Biosensor Mediated by CRISPR/Cas13a for Direct and Specific Detection of MiRNA-21 DOI Creative Commons
Yang Zhang, Pei Miao,

Jingyuan Wang

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(18), P. 6138 - 6138

Published: Sept. 23, 2024

Direct detection of miRNA is currently limited by the complex amplification and reverse transcription processes existing methods, leading to low sensitivity high operational demands. Herein, we developed a CRISPR/Cas13a-mediated photoelectrochemical (PEC) biosensing platform for direct sensitive miRNA-21. The specific recognition target miRNA-21 crRNA-21 eliminates need pre-amplification miRNA-21, thereby preventing signal distortion enhancing precision detection. When binds it activates trans-cleavage activity CRISPR/Cas13a, non-specific cleavage biotin-modified DNA with uracil bases (biotin-rU-DNA). This prevents biotin-rU-DNA from being immobilized on electrode surface. As result, streptavidin cannot attach via biotin binding, reducing spatial resistance causing positively correlated increase in photocurrent response. Cas-PEC biosensor has good analytical capabilities, linear responses between 10 fM nM, minimum limit 9 fM, an excellent recovery rate analysis real human serum samples. work presented innovative solution detecting other biomarkers bioanalysis clinical diagnostics.

Language: Английский

Citations

0