Development of Polyphenol–Metal Film-Modified Colored Porous Microspheres for Enhanced Monkeypox Antigen Detection DOI Creative Commons

Wei-Zhi Zhang,

Chenfei Zhang, Shou‐Nian Ding

et al.

Chemosensors, Journal Year: 2025, Volume and Issue: 13(4), P. 142 - 142

Published: April 12, 2025

The Monkeypox virus (MPXV), a DNA classified under the Orthpoxvirus genus alongside variola virus, has recently garnered significant global health attention due to its increasing transmission and emerging genomic mutations. Point-of-care testing is essential for effective clinical response outbreak mitigation. In this article, we developed novel class of colored microspheres designed application in lateral flow immunoassay (LFIA) platform targeting MPXV-specific biomarkers. Polystyrene-maleic anhydride (SMA-MAA) were synthesized with high-temperature soap-free emulsion polymerization optimized our lab. Subsequent alkali acid treatments employed introduce porosity into microsphere matrix. Solvent Red 27 Disperse 60 incorporated via solvent-swelling thermal-swelling methods, respectively, generate high brightness (HB) carriers. A surface coating composed tannic acid–iron (TA–Fe3⁺) coordination complex was applied form stable metal–polyphenol film (MPF). This not only minimized dye leaching by establishing robust shell but also improved distribution, thereby enhancing overall color intensity. final HB-LFIA system, configured sandwich format, demonstrated favorable sensitivity linear detection range antigen, indicating strong potential diagnostic use.

Language: Английский

A comprehensive review on progress and prospects of modified hydroxyapatite for uranium fixation from water DOI
Yanlin Chen, Qingyan Zhang,

Xijun Fu

et al.

Separation and Purification Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132599 - 132599

Published: March 1, 2025

Language: Английский

Citations

0
Development of Polyphenol–Metal Film-Modified Colored Porous Microspheres for Enhanced Monkeypox Antigen Detection DOI Creative Commons

Wei-Zhi Zhang,

Chenfei Zhang, Shou‐Nian Ding

et al.

Chemosensors, Journal Year: 2025, Volume and Issue: 13(4), P. 142 - 142

Published: April 12, 2025

The Monkeypox virus (MPXV), a DNA classified under the Orthpoxvirus genus alongside variola virus, has recently garnered significant global health attention due to its increasing transmission and emerging genomic mutations. Point-of-care testing is essential for effective clinical response outbreak mitigation. In this article, we developed novel class of colored microspheres designed application in lateral flow immunoassay (LFIA) platform targeting MPXV-specific biomarkers. Polystyrene-maleic anhydride (SMA-MAA) were synthesized with high-temperature soap-free emulsion polymerization optimized our lab. Subsequent alkali acid treatments employed introduce porosity into microsphere matrix. Solvent Red 27 Disperse 60 incorporated via solvent-swelling thermal-swelling methods, respectively, generate high brightness (HB) carriers. A surface coating composed tannic acid–iron (TA–Fe3⁺) coordination complex was applied form stable metal–polyphenol film (MPF). This not only minimized dye leaching by establishing robust shell but also improved distribution, thereby enhancing overall color intensity. final HB-LFIA system, configured sandwich format, demonstrated favorable sensitivity linear detection range antigen, indicating strong potential diagnostic use.

Language: Английский

Citations

0