Surfactant-assisted thermal hydrolysis of sewage sludge: Biomolecule extraction and its potential for protease production DOI Creative Commons
Luis Romero, Juan F. Moreno, Paula Oulego

et al.

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(5), P. 113353 - 113353

Published: June 18, 2024

Efficient management of sewage sludge from wastewater treatment plants (WWTPs) are crucial to obtain industrial value-added products and reducing environmental impact. Thermal hydrolysis, coupled with surfactants enhance solubilisation, offers a promising approach for treatment. This study assessed the impact four surfactants: sodium dodecyl sulphate (SDS), dodecylbenzenesulfonate (SDBS), cetyl trimethyl ammonium chloride (CTAC), tetraethylammonium (TAC) on thermal hydrolysis sludge. The addition anionic (SDS SDBS) significantly increased biomolecule production, proteins, humic-like substances, carbohydrates, DNA concentrations reaching 6018 ± 28 mg/L (306 mg/gVSSo), 2496 103 (127 1822 4 (93 389 3 (20 respectively, after 155 min hydrolysis. Low levels (10 50 mg/gVSSo) also led substantial increase in readily biodegradable fraction hydrolysed sludge, up 90 %. Additionally, surfactant-assisted proved be favourable substrate protease production using Bacillus licheniformis. optimal enzymatic activity 678 14 U/mL was achieved when CTAC added at concentration 10 mg/gVSSo. In summary, this evaluated an innovative solubilisation biomolecules methodology not only positively affected biodegradability but suitable potentially producing valuable like proteases through fermentation B. licheniformis, thus offering new strategy sustainable

Language: Английский

Performance of four thermophilic bacteria for primary sludge hydrolysis: Sludge disintegration and hydrolase activities DOI
Yonghao Liu,

Shangzong Li,

Dongxu Xing

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132123 - 132123

Published: Jan. 1, 2025

Language: Английский

Citations

0
Hydrolysed sewage sludge-derived solids as biosorbents for cationic dye removal DOI Creative Commons
Luis Romero, Paula Oulego, Sergio Collado

et al.

Separation and Purification Technology, Journal Year: 2025, Volume and Issue: unknown, P. 131479 - 131479

Published: Jan. 1, 2025

Language: Английский

Citations

0
Economic production of eco-friendly dehairing keratinase and antioxidant feather hydrolyzate using Bacillus halotolerans DOI Creative Commons
Shaymaa A. Ismail, Shaimaa A. Nour, El-Shahat H.A. Nashy

et al.

Biomass Conversion and Biorefinery, Journal Year: 2024, Volume and Issue: unknown

Published: July 3, 2024

Abstract Feather is a high recalcitrance keratin-based biomass, and keratin-degrading microorganisms have been seen as beneficial tools for its biodegradation production of valuable products with biomedical industrial applications. In the current study, isolated keratinolytic bacteria was identified on base 16 S rDNA sequencing data Bacillus halotolerans , efficiency in hydrolysis feather preservation most amino acid constituent estimated by high-performance liquid chromatography Pico-Tag (HPLC PICO-TAG) method. The produced hydrolysate possessed antioxidant activity 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging 52.3 ± 5.6 mmole TE/g without possessing any cytotoxicity to human normal skin fibroplast cell line BJ-1 MTT assay. Moreover, strain capable producing keratinase under submerged fermentation that optimized combining single-variable-at-a-time optimization application statistical designs (Plackett-Burman Box-Behnken). optimum 140.83 U/mL enhanced 3.2-fold initial activity, purified enzyme at pH 9.5 70 °C half-life 69.3 min. Finally, applicability dehairing bovine hide examined comparison sodium sulfide (Na 2 S) lime (Ca(OH) ) conventional results bio-treatment process, it achieved complete after h good quality manifested scanning electron microscope (SEM). addition, significant reduction pollution load parameters estimated.

Language: Английский

Citations

2
Enhancement of Livestock Wastewater Treatment by a Novel Wooden-modified Biocarrier DOI
Zhiwei Li,

Quan Feng,

Mingyi Lu

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: unknown, P. 125131 - 125131

Published: Oct. 1, 2024

Language: Английский

Citations

1
Surfactant-assisted thermal hydrolysis of sewage sludge: Biomolecule extraction and its potential for protease production DOI Creative Commons
Luis Romero, Juan F. Moreno, Paula Oulego

et al.

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(5), P. 113353 - 113353

Published: June 18, 2024

Efficient management of sewage sludge from wastewater treatment plants (WWTPs) are crucial to obtain industrial value-added products and reducing environmental impact. Thermal hydrolysis, coupled with surfactants enhance solubilisation, offers a promising approach for treatment. This study assessed the impact four surfactants: sodium dodecyl sulphate (SDS), dodecylbenzenesulfonate (SDBS), cetyl trimethyl ammonium chloride (CTAC), tetraethylammonium (TAC) on thermal hydrolysis sludge. The addition anionic (SDS SDBS) significantly increased biomolecule production, proteins, humic-like substances, carbohydrates, DNA concentrations reaching 6018 ± 28 mg/L (306 mg/gVSSo), 2496 103 (127 1822 4 (93 389 3 (20 respectively, after 155 min hydrolysis. Low levels (10 50 mg/gVSSo) also led substantial increase in readily biodegradable fraction hydrolysed sludge, up 90 %. Additionally, surfactant-assisted proved be favourable substrate protease production using Bacillus licheniformis. optimal enzymatic activity 678 14 U/mL was achieved when CTAC added at concentration 10 mg/gVSSo. In summary, this evaluated an innovative solubilisation biomolecules methodology not only positively affected biodegradability but suitable potentially producing valuable like proteases through fermentation B. licheniformis, thus offering new strategy sustainable

Language: Английский

Citations

1