Dual-mode action of scalable, high-quality engineered stem cell-derived SIRPα-extracellular vesicles for treating acute liver failure DOI Creative Commons
Seohyun Kim, Yoon Kyoung Kim, Seonghyun Kim

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 29, 2024

Abstract Acute liver failure (ALF) is a critical inflammatory condition characterized by rapid hepatocyte death, impaired regeneration due to the delayed removal of necroptotic cells, and high mortality rates. This study introduces novel dual-mode action therapeutic approach using extracellular vesicles expressing Signal Regulatory Protein Alpha (SIRP-EVs) derived from genetically engineered mesenchymal stem cells (MSCs). These SIRP-EVs are designed concurrently resolve necroptosis promote regeneration. Our studies identified CD47 SIRPα as promising targets for ALF. We developed scalable 3D bioreactor-based process that produces high-purity SIRP-EVs, which preserve MSC properties achieve significant production levels. target both macrophages hepatocytes in ALF models, enhancing macrophage phagocytic activity against via blockade promoting reprogramming with MSC-derived cargo. Comprehensive vitro vivo demonstrate decrease + leading reduced damage markers enhanced survival findings highlight potential ALF, offering prospects their application other diseases. Moreover, these results pave way advancing EV-based therapies toward clinical implementation.

Language: Английский

Recent advances in extracellular vesicles for therapeutic cargo delivery DOI Creative Commons
Hyo In Kim, Jin Bong Park, Yin Zhu

et al.

Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: 56(4), P. 836 - 849

Published: April 1, 2024

Abstract Exosomes, which are nanosized vesicles secreted by cells, attracting increasing interest in the field of biomedical research due to their unique properties, including biocompatibility, cargo loading capacity, and deep tissue penetration. They serve as natural signaling agents intercellular communication, inherent ability carry proteins, lipids, nucleic acids endows them with remarkable therapeutic potential. Thus, exosomes can be exploited for diverse applications, chemotherapy, gene therapy, photothermal therapy. Moreover, capacity homotypic targeting self-recognition provides opportunities personalized medicine. Despite advantages novel agents, there several challenges optimizing efficiency structural stability defining exosome origins. Future should include development large-scale, quality-controllable production methods, refinement drug strategies, extensive vivo studies clinical trials. unresolved difficulties, use efficient, stable, safe delivery systems is an interesting area research. Therefore, this review describes summarizes cutting-edge published high-impact journals that have introduced or enhanced effects using a system past 2 years. We provide informative overview current state research, highlighting properties applications exosomes. also emphasize future directions, underscoring importance addressing key issues field. With review, we encourage researchers further develop exosome-based drugs application, such may among most promising next-generation therapeutics.

Language: Английский

Citations

85

Engineered biological nanoparticles as nanotherapeutics for tumor immunomodulation DOI Creative Commons

Juwita N. Rahmat,

Jiayi Liu,

Taili Chen

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(11), P. 5862 - 5903

Published: Jan. 1, 2024

Schematic of the immunomodulatory approaches for tipping balance towards anti-cancer immunity. This image was created with BioRender.com.

Language: Английский

Citations

11

Controlled delivery of HIF-1α via extracellular vesicles with collagen-binding activity for enhanced wound healing DOI Creative Commons
Sungmi Jeon, Seongeon Cho,

Seongkyeong Yoo

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 380, P. 330 - 347

Published: Feb. 8, 2025

Language: Английский

Citations

2

Dual-mode action of scalable, high-quality engineered stem cell-derived SIRPα-extracellular vesicles for treating acute liver failure DOI Creative Commons
Seohyun Kim, Yoon Kyoung Kim, Seonghyun Kim

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 23, 2025

Language: Английский

Citations

1

Unravelling the role of extracellular vesicles in cervical cancer: Mechanisms of progression, resistance, and emerging therapeutic strategies DOI Creative Commons
Michelle van der Merwe, Kathryn H. Myburgh, Cathie Garnis

et al.

Gene, Journal Year: 2025, Volume and Issue: unknown, P. 149467 - 149467

Published: April 1, 2025

Language: Английский

Citations

1

Extracellular Vesicles-Based Cell-Cell Communication in Melanoma: New Perspectives in Diagnostics and Therapy DOI Open Access
Katarzyna Kluszczyńska, Małgorzata Czyż

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 965 - 965

Published: Jan. 4, 2023

Extracellular vesicles (EVs) are a heterogeneous group of cell-secreted particles that carry cargo functional biomolecules crucial for cell-to-cell communication with both physiological and pathophysiological consequences. In this review, we focus on evidence demonstrating the EV-mediated crosstalk between melanoma cells within tumor, immune stromal cells, promotes evasion influences all steps development from local progression, pre-metastatic niche formation, to metastatic colonization distant organs. We also discuss role EVs in resistance immunotherapy therapy BRAFV600/MEK inhibitors, shortly summarize recent advances potential applications diagnostics therapy.

Language: Английский

Citations

14

The Role of Extracellular Vesicles in Metabolic Diseases DOI Creative Commons
Carlos González-Blanco,

Sarai Iglesias-Fortes,

Ángela Cristina Lockwood

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(5), P. 992 - 992

Published: April 30, 2024

Extracellular vesicles represent a group of structures with the capacity to communicate different cells and organs. This complex network interactions can regulate multiple physiological processes in organism. Very importantly, these be altered during appearance diseases including cancer, metabolic diseases, etc. In addition, extracellular transport cargoes, altering initiation disease, driving progression, or even accelerating pathogenesis. Then, we have explored implication alterations such as pancreatic diabetes its complications non-alcoholic fatty liver disease. Finally, more detail communication between pancreas. summary, very efficient system for among tissues permit an biomarkers well being involved extracellular-vesicle-mediated molecules, serving potential therapy diseases.

Language: Английский

Citations

5

Hypoxic regulation of extracellular vesicles: Implications for cancer therapy DOI

Seongkyeong Yoo,

Sanga Choi,

Iljin Kim

et al.

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 363, P. 201 - 220

Published: Sept. 28, 2023

Language: Английский

Citations

12

Extracellular Vesicles as Delivery Systems in Disease Therapy DOI Open Access

Manuel Alejandro Picon,

Liyong Wang, Andrea da Fonseca Ferreira

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(24), P. 17134 - 17134

Published: Dec. 5, 2023

Extracellular vesicles (EVs)/exosomes are nanosized membrane-bound structures that released by virtually all cells. EVs have attracted great attention in the scientific community since discovery of their roles cell-to-cell communication. EVs’ enclosed structure protects bioactive molecules from degradation extracellular space and targets specific tissues according to topography membrane proteins. Upon absorption recipient cells, EV cargo can modify transcription machinery alter cellular functions these playing a role disease pathogenesis. been tested as delivery system for mRNA COVID-19 vaccine. Recently, different therapeutic strategies designed use microRNAs mRNA. In this review, we will focus on exciting various platforms related using vehicles, mainly gene editing CRISPR/Cas9, cancer therapy, drug delivery, vaccines. We also touch upon

Language: Английский

Citations

12

Recent progress in engineered extracellular vesicles and their biomedical applications DOI
Farhan Mazahir, Awesh K. Yadav

Life Sciences, Journal Year: 2024, Volume and Issue: 350, P. 122747 - 122747

Published: May 24, 2024

Language: Английский

Citations

4