bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 29, 2024
Abstract
Acute
liver
failure
(ALF)
is
a
critical
inflammatory
condition
characterized
by
rapid
hepatocyte
death,
impaired
regeneration
due
to
the
delayed
removal
of
necroptotic
cells,
and
high
mortality
rates.
This
study
introduces
novel
dual-mode
action
therapeutic
approach
using
extracellular
vesicles
expressing
Signal
Regulatory
Protein
Alpha
(SIRP-EVs)
derived
from
genetically
engineered
mesenchymal
stem
cells
(MSCs).
These
SIRP-EVs
are
designed
concurrently
resolve
necroptosis
promote
regeneration.
Our
studies
identified
CD47
SIRPα
as
promising
targets
for
ALF.
We
developed
scalable
3D
bioreactor-based
process
that
produces
high-purity
SIRP-EVs,
which
preserve
MSC
properties
achieve
significant
production
levels.
target
both
macrophages
hepatocytes
in
ALF
models,
enhancing
macrophage
phagocytic
activity
against
via
blockade
promoting
reprogramming
with
MSC-derived
cargo.
Comprehensive
vitro
vivo
demonstrate
decrease
+
leading
reduced
damage
markers
enhanced
survival
findings
highlight
potential
ALF,
offering
prospects
their
application
other
diseases.
Moreover,
these
results
pave
way
advancing
EV-based
therapies
toward
clinical
implementation.
Experimental & Molecular Medicine,
Journal Year:
2024,
Volume and Issue:
56(4), P. 836 - 849
Published: April 1, 2024
Abstract
Exosomes,
which
are
nanosized
vesicles
secreted
by
cells,
attracting
increasing
interest
in
the
field
of
biomedical
research
due
to
their
unique
properties,
including
biocompatibility,
cargo
loading
capacity,
and
deep
tissue
penetration.
They
serve
as
natural
signaling
agents
intercellular
communication,
inherent
ability
carry
proteins,
lipids,
nucleic
acids
endows
them
with
remarkable
therapeutic
potential.
Thus,
exosomes
can
be
exploited
for
diverse
applications,
chemotherapy,
gene
therapy,
photothermal
therapy.
Moreover,
capacity
homotypic
targeting
self-recognition
provides
opportunities
personalized
medicine.
Despite
advantages
novel
agents,
there
several
challenges
optimizing
efficiency
structural
stability
defining
exosome
origins.
Future
should
include
development
large-scale,
quality-controllable
production
methods,
refinement
drug
strategies,
extensive
vivo
studies
clinical
trials.
unresolved
difficulties,
use
efficient,
stable,
safe
delivery
systems
is
an
interesting
area
research.
Therefore,
this
review
describes
summarizes
cutting-edge
published
high-impact
journals
that
have
introduced
or
enhanced
effects
using
a
system
past
2
years.
We
provide
informative
overview
current
state
research,
highlighting
properties
applications
exosomes.
also
emphasize
future
directions,
underscoring
importance
addressing
key
issues
field.
With
review,
we
encourage
researchers
further
develop
exosome-based
drugs
application,
such
may
among
most
promising
next-generation
therapeutics.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(2), P. 965 - 965
Published: Jan. 4, 2023
Extracellular
vesicles
(EVs)
are
a
heterogeneous
group
of
cell-secreted
particles
that
carry
cargo
functional
biomolecules
crucial
for
cell-to-cell
communication
with
both
physiological
and
pathophysiological
consequences.
In
this
review,
we
focus
on
evidence
demonstrating
the
EV-mediated
crosstalk
between
melanoma
cells
within
tumor,
immune
stromal
cells,
promotes
evasion
influences
all
steps
development
from
local
progression,
pre-metastatic
niche
formation,
to
metastatic
colonization
distant
organs.
We
also
discuss
role
EVs
in
resistance
immunotherapy
therapy
BRAFV600/MEK
inhibitors,
shortly
summarize
recent
advances
potential
applications
diagnostics
therapy.
Biomedicines,
Journal Year:
2024,
Volume and Issue:
12(5), P. 992 - 992
Published: April 30, 2024
Extracellular
vesicles
represent
a
group
of
structures
with
the
capacity
to
communicate
different
cells
and
organs.
This
complex
network
interactions
can
regulate
multiple
physiological
processes
in
organism.
Very
importantly,
these
be
altered
during
appearance
diseases
including
cancer,
metabolic
diseases,
etc.
In
addition,
extracellular
transport
cargoes,
altering
initiation
disease,
driving
progression,
or
even
accelerating
pathogenesis.
Then,
we
have
explored
implication
alterations
such
as
pancreatic
diabetes
its
complications
non-alcoholic
fatty
liver
disease.
Finally,
more
detail
communication
between
pancreas.
summary,
very
efficient
system
for
among
tissues
permit
an
biomarkers
well
being
involved
extracellular-vesicle-mediated
molecules,
serving
potential
therapy
diseases.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(24), P. 17134 - 17134
Published: Dec. 5, 2023
Extracellular
vesicles
(EVs)/exosomes
are
nanosized
membrane-bound
structures
that
released
by
virtually
all
cells.
EVs
have
attracted
great
attention
in
the
scientific
community
since
discovery
of
their
roles
cell-to-cell
communication.
EVs’
enclosed
structure
protects
bioactive
molecules
from
degradation
extracellular
space
and
targets
specific
tissues
according
to
topography
membrane
proteins.
Upon
absorption
recipient
cells,
EV
cargo
can
modify
transcription
machinery
alter
cellular
functions
these
playing
a
role
disease
pathogenesis.
been
tested
as
delivery
system
for
mRNA
COVID-19
vaccine.
Recently,
different
therapeutic
strategies
designed
use
microRNAs
mRNA.
In
this
review,
we
will
focus
on
exciting
various
platforms
related
using
vehicles,
mainly
gene
editing
CRISPR/Cas9,
cancer
therapy,
drug
delivery,
vaccines.
We
also
touch
upon
