Phage therapeutic delivery methods and clinical trials for combating clinically relevant pathogens DOI
Heba Mohammed Refat M. Selim, Fatma Alzahraa M. Gomaa, Mohammad Y. Alshahrani

et al.

Therapeutic Delivery, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 23

Published: Nov. 15, 2024

The ongoing global health crisis caused by multidrug-resistant (MDR) bacteria necessitates quick interventions to introduce new management strategies for MDR-associated infections and antimicrobial agents' resistance. Phage therapy emerges as an antibiotic substitute its high specificity, efficacy, safety profiles in treating infections. Various vitro vivo studies denoted their eminent bactericidal anti-biofilm potential. This review addresses the latest developments phage regarding attack strategies, formulations, administration routes. It additionally discusses elaborates on status of undergoing clinical trials, challenges encountered usage, explores prospects research application

Language: Английский

Phage therapy DOI
Mikael Skurnik,

Sivan Alkalay‐Oren,

Maarten Boon

et al.

Nature Reviews Methods Primers, Journal Year: 2025, Volume and Issue: 5(1)

Published: Feb. 13, 2025

Citations

2
Targeting Pseudomonas aeruginosa biofilm with an evolutionary trained bacteriophage cocktail exploiting phage resistance trade-offs DOI Creative Commons

Fabian Kunisch,

Claudia Campobasso, Jeroen Wagemans

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 3, 2024

Language: Английский

Citations

9
Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches DOI Creative Commons
Minyoung Kevin Kim, Gina A. Suh, Grace Cullen

et al.

Journal of Clinical Investigation, Journal Year: 2025, Volume and Issue: 135(5)

Published: March 2, 2025

Bacteriophage (phage) therapy has emerged as a promising solution to combat the growing crisis of multidrug-resistant (MDR) infections. There are several international centers actively engaged in implementation phage therapy, and recent case series have reported encouraging success rates patients receiving personalized, compassionate for difficult-to-treat Nonetheless, substantial hurdles remain way more widespread adoption consistent success. This Review offers comprehensive overview current technologies therapeutic approaches. We first delineate common steps development, from bank establishment clinical administration, examine spectrum approaches, personalized fixed cocktails. Using framework conventional drug development pipeline, we then identify critical knowledge gaps areas such cocktail design, formulation, pharmacology, trial design. conclude that, while holds promise, structured pipeline sustained government support crucial MDR

Language: Английский

Citations

1
Phage Delivery Strategies for Biocontrolling Human, Animal, and Plant Bacterial Infections: State of the Art DOI Creative Commons
Marta M. D. C. Vila,

Liliane M. N. Balcão,

Victor M. Balcão

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(3), P. 374 - 374

Published: March 8, 2024

This review aims at presenting the main strategies that are currently available for delivery of bacteriophages to combat bacterial infections in humans, animals, and plants. It can be seen routes phage topical, oral, systemic, airways humans. In topical oral most used. To plant species, spraying plant’s phyllosphere or drenching soil commonly used methods. both therapy biocontrol using phages, very promising results have been obtained so far. However, more experiments needed establish forms treatment doses, among other parameters. Furthermore, general, there is a lack specific standards use phages infections.

Language: Английский

Citations

7
Phage-Based antibacterial hydrogels for bacterial targeting and Ablation: Progress and perspective DOI
Haoran Bai,

Qinggele Borjihan,

Zheng Li

et al.

European Journal of Pharmaceutics and Biopharmaceutics, Journal Year: 2024, Volume and Issue: 198, P. 114258 - 114258

Published: March 12, 2024

Language: Английский

Citations

5
Combination of bacteriophages and vancomycin in a co-delivery hydrogel for localized treatment of fracture-related infections DOI Creative Commons
Baixing Chen,

Luis Ponce Benavente,

Marco Chittò

et al.

npj Biofilms and Microbiomes, Journal Year: 2024, Volume and Issue: 10(1)

Published: Aug. 29, 2024

Fracture-related infections (FRIs), particularly those caused by methicillin-resistant Staphylococcus aureus (MRSA), are challenging to treat. This study designed and evaluated a hydrogel loaded with cocktail of bacteriophages vancomycin (1.2 mg/mL). The co-delivery showed 99.72% reduction in MRSA biofilm vitro. released 54% phages 82% within 72 h maintained activity for eight days, vivo the systemic antibiotic significantly reduced bacterial load 0.99 log10 CFU compared controls, active detected tissues at euthanasia (2 × 103 PFU/mL). No phage resistance was treatment groups, serum neutralization resulted only 20% count. In this work, we show that phage-antibiotic system via CMC is promising adjunct therapy MRSA-induced FRI, highlighting its potential localized, sustained delivery improved outcomes.

Language: Английский

Citations

5
Bacteriophage-mediated approaches for biofilm control DOI Creative Commons
Arianna Mayorga-Ramos, Saskya E. Carrera-Pacheco, Carlos Barba‐Ostria

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Oct. 7, 2024

Biofilms are complex microbial communities in which planktonic and dormant bacteria enveloped extracellular polymeric substances (EPS) such as exopolysaccharides, proteins, lipids, DNA. These multicellular structures present resistance to conventional antimicrobial treatments, including antibiotics. The formation of biofilms raises considerable concern healthcare settings, can exacerbate infections patients compromise the integrity medical devices employed during treatment. Similarly, certain bacterial species contribute bulking, foaming, biofilm development water environments wastewater treatment plants, reservoirs, aquaculture facilities. Additionally, food production facilities provide ideal conditions for establishing biofilms, serve reservoirs foodborne pathogens. Efforts combat antibiotic involve exploring various strategies, bacteriophage therapy. Research has been conducted on effects phages their individual proteins assess potential removal. However, challenges persist, prompting examination refined approaches drug-phage combination therapies, phage cocktails, genetically modified clinical applications. This review aims highlight progress regarding bacteriophage-based eradication different settings.

Language: Английский

Citations

5
Synergistic effects of bacteriophage cocktail and antibiotics combinations against extensively drug-resistant Acinetobacter baumannii DOI Creative Commons
Sanaz Rastegar, Mikael Skurnik, Omid Tadjrobehkar

et al.

BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 26, 2024

The extensively drug-resistant (XDR) strains of Acinetobacter baumannii have become a major cause nosocomial infections, increasing morbidity and mortality worldwide. Many different treatments, including phage therapy, are attractive ways to overcome the challenges antibiotic resistance. This study investigates biofilm formation ability 30 XDR A. isolates efficacy cocktail four tempetate bacteriophages (SA1, Eve, Ftm, Gln) antibiotics (ampicillin/sulbactam, meropenem, colistin) in inhibiting degrading biofilms these strains. majority (83.3%) exhibited strong formation. bacteriophage showed varying degrees effectiveness against biofilms, with higher concentrations generally leading more significant inhibition degradation rates. antibiotics-bacteriophage combinations also enhanced biofilms. findings suggested that is an effective tool combating its depending on concentration. Combining improved removal indicating promising strategy for managing infections. These results contribute our understanding dynamics potential cocktails as novel therapeutic approach combat antibiotic-resistant bacteria.

Language: Английский

Citations

5
Novel Antibacterial Agents and Emerging Therapies in the Fight Against Multidrug-Resistant Acinetobacter baumannii DOI
Elijah Kolawole Oladipo, Stephen Feranmi Adeyemo,

Glory Jesudara Oluwasanya

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: 200, P. 107361 - 107361

Published: Jan. 31, 2025

Language: Английский

Citations

0
The landscape of biofilm models for phage therapy: mimicking biofilms in diabetic foot ulcers using 3D models DOI Creative Commons
Mark Grevsen Martinet, Marvin L. Thomas,

Jörg Bojunga

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 24, 2025

Diabetic foot ulcers (DFU) affect up to 15-25% of patients suffering from diabetes and are considered a global health concern. These may result in delayed wound healing chronic infections, with the potential lead amputations. It has been estimated that 85% diabetes-related amputations preceded by diagnosis DFU. A critical factor persistence this disease is presence polymicrobial biofilms, which generally include Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli. The involvement diabetic comorbidities such as ischemia, hyperglycemia, immune-compromised status creates perfect niche for these bacteria evade body's immune response persist biofilms. Bacteriophage therapy can target lyse specific emerging an effective treatment biofilm-related infections. While shows promise addressing wounds, our current models, including animal static systems, fail capture full complexity Innovative approaches 3D bioengineered skin organoid hydrogel-based systems being developed simulate DFU microenvironments more accurately without using ex vivo or tissues. advanced models evaluating bacteriophage efficacy biofilm-associated DFU, aiming enhance preclinical assessments improve therapeutic outcomes patients.

Language: Английский

Citations

0