Alternative drug delivery systems for pregnancy related disorders: A focused review DOI Creative Commons
Ananth Kumar Kammala, Ryan C. V. Lintao, Ramkumar Menon

et al.

Placenta and Reproductive Medicine, Journal Year: 2024, Volume and Issue: 3

Published: Aug. 6, 2024

Objectives: The objective of this review was to summarize currently developed advanced drug delivery systems that can be used for pregnancy related disorders along with their advantages and clinical relevance. Methods: This process a defined search strategy indexed publications from databases like Google scholar, PubMed, Embase, Medline, Scopus UTMB library 2000 2020 included methods assess the quality evidence retrieved. Results: Pregnancy is unique physiological condition where some drugs will lifesaving mother, but they toxic fetal tissue. It mandatory research on novel systems. Although there substantial quantity has been carried out maternal -placental-fetal uptake, transfer, toxicity, therapeutic evaluations in remain infancy. Conclusions: In review, we highlighted considerations placenta summarized recent work as well potential applications reality.

Language: Английский

Targeted delivery systems of siRNA based on ionizable lipid nanoparticles and cationic polymer vectors DOI
Zhao Yao,

Taiqing Liu,

Jingwen Wang

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: 81, P. 108546 - 108546

Published: Feb. 26, 2025

Language: Английский

Citations

2
Placenta-tropic VEGF mRNA lipid nanoparticles ameliorate murine pre-eclampsia DOI
Kelsey L. Swingle, Alex G. Hamilton, Hannah C. Safford

et al.

Nature, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Language: Английский

Citations

11
Modular Design of Lipopeptide‐Based Organ‐Specific Targeting (POST) Lipid Nanoparticles for Highly Efficient RNA Delivery DOI Open Access

Chuanmei Tang,

Yexi Zhang,

Bowen Li

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Lipid nanoparticles (LNPs) with highly efficient and specific extrahepatic targeting abilities are promising in gene delivery, the lipopeptides (LPs) excellent designability functionality expected to empower construction of functional LNPs. This study aims develop ionizable components that accurately match different lipid systems through modular design LPs. Based on this, a lipopeptide-based organ-specific (POST) LNP screening strategy is constructed, which lysine-histidine-based (KH-LPs) designed as components. The optimal KH-LP screened vitro shows siRNA/mRNA transfecting ability various hard-to-transfect cell lines. Compared classic LNPs, POST LNPs vivo achieve even higher (or at least comparable) efficiency specificity delivering mRNA siRNA lung, liver, spleen, respectively. structure-activity relationship (SAR) proves regulation LP structures can provide for systems, demonstrating potential this developing selective open up more possibilities therapy.

Language: Английский

Citations

1
EGF receptor in organ development, tissue homeostasis and regeneration DOI Creative Commons

Claudia Tito,

Silvia Masciarelli, Gianni Colotti

et al.

Journal of Biomedical Science, Journal Year: 2025, Volume and Issue: 32(1)

Published: Feb. 19, 2025

Abstract The epidermal growth factor receptor (EGFR) is a protein embedded in the outer membrane of epithelial and mesenchymal cells, bone blood immune heart glia stem neural cells. It belongs to ErbB family, which includes three other related proteins: HER2/ErbB2/c-neu, HER3/ErbB3, HER4/ErbB4. EGFR binds seven known signaling molecules, including (EGF) transforming factor-alpha (TGF-α). This binding triggers formation pairs (dimers), self-phosphorylation EGFR, activation several pathways within cell. These influence various cellular processes like proliferation, differentiation, migration, survival. plays critical role both development tissue homeostasis, repair adult organ regeneration. Altered expression linked disruption homeostasis diseases, among cancer. review focuses on how contributes different organs placenta, gut, liver, bone, skin, brain, T cell regulation, pancreas, kidneys, mammary glands lungs along with their associated pathologies. involvement organ-specific branching morphogenesis process also discussed. level activity its impact vary across organs. Factors as affinity ligands, recycling or degradation processes, transactivation by proteins environmental factors (such heat stress smoking) play regulating activity. Understanding EGFR’s regulatory mechanisms holds promise for developing targeted therapeutic strategies.

Language: Английский

Citations

1
Nanomaterials in the diagnosis and treatment of gastrointestinal tumors: New clinical choices and treatment strategies DOI Creative Commons
Liping Chen,

Qingqing Li

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101782 - 101782

Published: April 19, 2025

Language: Английский

Citations

1
Lipid nanoparticles for nucleic acid delivery beyond the liver DOI

Nadine Saber,

Mariona Estapé Sentí,

Raymond M. Schiffelers

et al.

Human Gene Therapy, Journal Year: 2024, Volume and Issue: 35(17-18), P. 617 - 627

Published: Aug. 14, 2024

Lipid nanoparticles (LNPs) are the most clinically advanced drug delivery system for nucleic acid therapeutics, exemplified by success of COVID-19 mRNA vaccines. However, their clinical use is currently limited to hepatic diseases and vaccines due tendency accumulate in liver upon intravenous administration. To fully leverage potential, it essential understand address tropism, while also developing strategies enhance tissues beyond liver. Ensuring that these therapeutics reach target cells avoiding off-target both efficacy safety. There three potential targeting strategies-passive, active, endogenous-which can be used individually or combination nonhepatic tissues. In this review, we delve into recent advancements LNP engineering delivering

Language: Английский

Citations

6
Peptide-Functionalized Lipid Nanoparticles for Targeted Systemic mRNA Delivery to the Brain DOI
Emily L. Han, Sophia Tang, Dongyoon Kim

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Systemic delivery of large nucleic acids, such as mRNA, to the brain remains challenging in part due blood-brain barrier (BBB) and tendency vehicles accumulate liver. Here, we design a peptide-functionalized lipid nanoparticle (LNP) platform for targeted mRNA brain. We utilize click chemistry functionalize LNPs with peptides that target receptors overexpressed on endothelial cells neurons, namely RVG29, T7, AP2, mApoE peptides. evaluate effect LNP targeting neuronal cell transfection vitro, investigating factors serum protein adsorption, intracellular trafficking, transcytosis, exosome secretion. Finally, show peptide functionalization enhances mouse reduces hepatic after systemic administration. Specifically, RVG29 improved vivo, establishing its potential nonviral delivering

Language: Английский

Citations

6
Optimizing lipid nanoparticles for fetal gene delivery in vitro, ex vivo, and aided with machine learning DOI Creative Commons

Amr Abostait,

Mahmoud Abdelkarim, Zeqing Bao

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 376, P. 678 - 700

Published: Oct. 28, 2024

There is a clinical need to develop lipid nanoparticles (LNPs) deliver congenital therapies the fetus during pregnancy. The aim of these restore normal fetal development and prevent irreversible conditions after birth. As first step, LNPs be optimized for transplacental transport, safety on placental barrier organs transfection efficiency. We developed characterized library varying compositions used machine learning (ML) models delineate determinants LNP size zeta potential. Utilizing different in vitro with help Random Forest algorithm, we could identify top features driving percentage transport kinetics at 24 h, out total 18 input represented by 41 formulations 48 experiments. further evaluated safety, cell uptake, efficiency trophoblasts lung fibroblasts. To ensure integrity following screened using high-throughput integrated transport-transfection model. Finally, assessed toxicity tracheal occlusion explant showed little no cells. Immunoglobin G (IgG) orientation surface LNPs, PEGylated lipids, ionizable lipids had significant effects transport. algorithm identified kinetics. Zeta potential emerged features. Building ML model results, new optimize leading 622 % increase h versus control formulation. induce preferential siRNA lung, cationic lipid-to-siRNA ratio. Studying an fibroblasts strong correlation between transfection. appeared safe ex vivo lungs as indicated insignificant changes apoptosis (Caspase-3) proliferation (Ki67) markers. In conclusion, have formulation that safe, high Our research findings represent important step toward establishing effectiveness gene delivery organs.

Language: Английский

Citations

6
Potential use of nanoparticles to improve the effectiveness and safety of pharmacotherapy during pregnancy DOI Creative Commons
Ksenia Zagorodnikova, Ofelia A. Bettikher, И. Е. Зазерская

et al.

Kachestvennaya Klinicheskaya Praktika = Good Clinical Practice, Journal Year: 2025, Volume and Issue: 4, P. 35 - 45

Published: Jan. 17, 2025

Relevance . Pharmacotherapy during pregnancy is associated with great risks for the fetus in case of therapy non-obstetric diseases mother, addition, issues improving effectiveness treatment placental pathology, thrombophilic conditions, and other complications remain a priority. Recently, possibilities using nanomaterials medicine have been actively studied, including purpose modifying delivery or distribution medicines. Objective The aim was to conduct structured analysis published literature on use therapeutic purposes pregnancy. Methods publications PubMed, Cyberleninka, eLIBRARY databases keywords “Nanoparticles” “Pregnancy” carried out, exception mentioning nanoparticles context environmental pollutants, as well diagnostic methods. Results Information provided nature that developed studied prospect An overview areas clinical application such materials provided, available evidence their safety. Conclusion Therapeutic systems containing drugs various good potential order ensure targeted drug delivery, modification bio-distribution increase efficacy reduce toxicity drugs.

Language: Английский

Citations

0
Apolipoprotein Fusion Enables Spontaneous Functionalization of mRNA Lipid Nanoparticles with Antibody for Targeted Cancer Therapy DOI Creative Commons
Wonbeom Park, Jiwoong Choi, Jaehyeon Hwang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

The mRNA-lipid nanoparticles (mRNA@LNPs) offer a novel opportunity to treat targets previously considered undruggable. Although antibody conjugation is crucial for enhancing the specificity, delivery efficiency, and minimizing toxicity of mRNA therapeutics, current chemical methods are complex produce heterogeneous particles with misoriented antibodies. In this work, we introduce chemical-free approach functionalize mRNA@LNPs antibodies, mimicking protein corona formation targeted delivery. By fusing apolipoprotein Fc domain targeting antibody, enabled spontaneously display on surface without altering existing LNP process or employing techniques. We demonstrated precise expression using trastuzumab-bound mRNA@LNPs, facilitating specific in HER2-positive cancer cells. was efficiently delivered tumor site after intravenous administration. While control LNPs lacking antibodies caused acute liver toxicity, trastuzumab-displayed showed no systemic toxicity. tumor-specific p53 suppressor led complete regression Thus, fusion enables straightforward scalable production antibody-functionalized offering significant therapeutic potential gene therapy.

Language: Английский

Citations

0