
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 11, 2024
Language: Английский
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 11, 2024
Language: Английский
JCI Insight, Journal Year: 2025, Volume and Issue: 10(2)
Published: Jan. 22, 2025
Diabetes mellitus can cause impaired and delayed wound healing, leading to lower extremity amputations; however, the mechanisms underlying regulation of vascular endothelial growth factor-dependent (VEGF-dependent) angiogenesis remain unclear. In our study, molecular underpinnings dysfunction in diabetes are investigated, focusing on roles disabled-2 (Dab2) Forkhead box M1 (FOXM1) VEGF receptor 2 (VEGFR2) signaling cell function. Bulk RNA-sequencing analysis identified significant downregulation Dab2 high-glucose-treated primary mouse skin cells. diabetic mice with deficiency Dab2, vivo vitro healing were reduced when compared wild-type mice. Restoration expression by injected mRNA-containing, LyP-1-conjugated lipid nanoparticles rescued Furthermore, FOXM1 was downregulated cells under high-glucose conditions as determined analysis. found bind promoter, regulating its influencing VEGFR2 signaling. The inhibitor FDI-6 phosphorylation VEGFR2. Our study provides evidence crucial establishes targeted delivery a promising treatment for complications.
Language: Английский
Citations
2Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 380, P. 330 - 347
Published: Feb. 8, 2025
Language: Английский
Citations
2Plasma Chemistry and Plasma Processing, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
1Bioactive Materials, Journal Year: 2025, Volume and Issue: 47, P. 1 - 17
Published: Jan. 8, 2025
Language: Английский
Citations
0Aging Cell, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 23, 2025
ABSTRACT Telomeres shorten with each cell division, acting as a chronometer of age. The enzyme telomerase, primarily active in stem cells, reverses telomere erosion. We have previously observed that transient transfection human TERT mRNA extends telomeres and mitigates hallmarks senescence replicatively aged cells or those affected by Hutchinson–Gilford progeroid syndrome (HGPS). However, due to its short half‐life, requires frequent administration. In this study, we hypothesized circular (circ) RNA would extend the duration telomerase expression be more effective at reversing endothelial derived from HGPS patients. observe single circRNA is than extension length, determined quantitative fluorescence situ hybridization. Furthermore, reduced number β‐gal positive three‐fold normalized nuclear morphology (HGPS‐ECs). Moreover, substantially senescent markers, inflammatory DNA damage including Progerin, p16, p21, IL‐1B, IL‐6, IL‐8, MCP1, γH2AX. Additionally, it restored NO production, enhanced proliferation, promoted angiogenesis, improved LDL uptake, mitochondrial ROS, membrane potential effectively. Our data suggest superior linear processes involved senescence.
Language: Английский
Citations
0Nano Today, Journal Year: 2025, Volume and Issue: 62, P. 102739 - 102739
Published: April 1, 2025
Language: Английский
Citations
0Injury, Journal Year: 2025, Volume and Issue: 56(6), P. 112314 - 112314
Published: April 6, 2025
Language: Английский
Citations
0Published: April 1, 2025
Language: Английский
Citations
0Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown
Published: April 27, 2025
Abstract Atrophic nonunion, a particularly challenging complication of diaphyseal bone fractures, arises from deteriorated regenerative microenvironment characterized by insufficient vascularization and pathological accumulation reactive oxygen species (ROS). To address this clinical challenge, dual‐function bioactive scaffolds is developed that simultaneously blocks disease progression promotes tissue regeneration. The design incorporates three key components: First, MnO 2 ‐Cu 2+ (MC) nanoparticles are synthesized to combat the through dual mechanisms ROS scavenging angiogenesis promotion. Second, circular morphogenetic protein (circBMP2) engineered for sustained BMP2 expression enhance osteogenic differentiation, with polyethylenimine‐mediated surface conjugation onto MC nanoparticles. Finally, these MC‐circBMP2 (MCB) complexes integrated into dopamine‐modified demineralized matrix create hierarchically structured platform. In vivo experiments showed promoted healing atrophic nonunion radial defects in rabbits improving angiogenesis, ROS, accelerating differentiation endogenous cells. above results indicate designed can precisely block pathology rapidly initiate regeneration damaged tissue, providing theoretical guidance refractory injuries.
Language: Английский
Citations
0International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 5837 - 5857
Published: May 1, 2025
Diabetic foot ulcers (DFU) represent one of the most common side effects diabetes, significantly impacting patients' quality life and imposing considerable financial burdens on families society at large. Despite advancements in therapies targeting lower limb revascularization various medications dressings, outcomes for patients with severe lesions remain limited. A recent breakthrough DFU treatment stems from development mesenchymal stem cells (MSCs). MSCs have shown promising results treating diseases skin wounds due to their ability multidirectional differentiation immunomodulation. Recent studies highlight that primarily repair tissue through paracrine activities, exosomes playing a crucial role as main biologically active components. These transport proteins, mRNA, DNA, other substances, facilitating immunomodulation, antioxidant effects, angiogenesis promotion, endothelial cell migration proliferation, collagen remodeling. Mesenchymal cell-derived (MSC-Exo) not only deliver comparable therapeutic but also mitigate adverse reactions like immune rejection associated transplantation. This article provides an overview pathophysiology explores mechanisms research progress MSC-Exo therapy.
Language: Английский
Citations
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