Irradiation with cold atmospheric direct plasma: an innovative approach to treating murine cutaneous wounds DOI Creative Commons
Baida M. Ahmed, Sahar A. H. Al-Sharqi, Madyan A. Khalaf

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Abstract Cold atmospheric direct plasma (CADP), an ionized gas at ambient temperature, represents a promising approach to enhancing tissue regeneration. A laboratory-based study was conducted investigate the effects of medical CADP on reparative potential full-thickness acute skin wounds in murine models. For vivo investigations, two dermal injuries were induced each subject, with diameter approximately 8 mm (n = 20). We employed floating electrode within system that generates pressure air plasma, characterized by temperature ranging from 36 38°C. The received three treatments, administered twice daily for irradiation durations 5, 15, and 25 seconds. These subsequently evaluated intervals 2, 4, 6, 8, 11 days post-wounding through histological examination gene expression analysis. On eleventh day, wound healing rates recorded 34.80% control group, while plasma-treated groups achieved 56.62%, 84.97%, 97.82%, respectively. Histological revealed plasma-treatment promotes development epidermal keratin granular strata, along formation hair follicles sebaceous glands. Gene analysis indicates increased levels growth factors decrease white blood cell counts. therapeutic intervention has significantly enhanced efficacy dermatological lesions without any noticeable adverse or simultaneous activation pro-inflammatory signaling pathways. findings underscore benefits employing applications management clinical settings.

Language: Английский

Endothelial FOXM1 and Dab2 promote diabetic wound healing DOI Creative Commons
Sudarshan Bhattacharjee,

Jianing Gao,

Yao Wei Lu

et al.

JCI Insight, Journal Year: 2025, Volume and Issue: 10(2)

Published: Jan. 22, 2025

Diabetes mellitus can cause impaired and delayed wound healing, leading to lower extremity amputations; however, the mechanisms underlying regulation of vascular endothelial growth factor-dependent (VEGF-dependent) angiogenesis remain unclear. In our study, molecular underpinnings dysfunction in diabetes are investigated, focusing on roles disabled-2 (Dab2) Forkhead box M1 (FOXM1) VEGF receptor 2 (VEGFR2) signaling cell function. Bulk RNA-sequencing analysis identified significant downregulation Dab2 high-glucose-treated primary mouse skin cells. diabetic mice with deficiency Dab2, vivo vitro healing were reduced when compared wild-type mice. Restoration expression by injected mRNA-containing, LyP-1-conjugated lipid nanoparticles rescued Furthermore, FOXM1 was downregulated cells under high-glucose conditions as determined analysis. found bind promoter, regulating its influencing VEGFR2 signaling. The inhibitor FDI-6 phosphorylation VEGFR2. Our study provides evidence crucial establishes targeted delivery a promising treatment for complications.

Language: Английский

Citations

2
Controlled delivery of HIF-1α via extracellular vesicles with collagen-binding activity for enhanced wound healing DOI Creative Commons
Sungmi Jeon, Seongeon Cho,

Seongkyeong Yoo

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 380, P. 330 - 347

Published: Feb. 8, 2025

Language: Английский

Citations

2
Irradiation With Cold Atmospheric Direct Plasma: An Innovative Approach to Treating Murine Cutaneous Wounds DOI
Madyan A. Khalaf, Baida M. Ahmed, Sahar A. H. Al-Sharqi

et al.

Plasma Chemistry and Plasma Processing, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

1
Dermal fibroblast-derived extracellular matrix (ECM) synergizes with keratinocytes in promoting re-epithelization and scarless healing of skin wounds: Towards optimized skin tissue engineering DOI
Xiangyu Dong,

Han Xiang,

Jiajia Li

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 47, P. 1 - 17

Published: Jan. 8, 2025

Language: Английский

Citations

0
Circular RNA Telomerase Reverses Endothelial Senescence in Progeria DOI Creative Commons

Wei-Feng Qin,

Kathrina Castillo,

Haoju Li

et al.

Aging Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 23, 2025

ABSTRACT Telomeres shorten with each cell division, acting as a chronometer of age. The enzyme telomerase, primarily active in stem cells, reverses telomere erosion. We have previously observed that transient transfection human TERT mRNA extends telomeres and mitigates hallmarks senescence replicatively aged cells or those affected by Hutchinson–Gilford progeroid syndrome (HGPS). However, due to its short half‐life, requires frequent administration. In this study, we hypothesized circular (circ) RNA would extend the duration telomerase expression be more effective at reversing endothelial derived from HGPS patients. observe single circRNA is than extension length, determined quantitative fluorescence situ hybridization. Furthermore, reduced number β‐gal positive three‐fold normalized nuclear morphology (HGPS‐ECs). Moreover, substantially senescent markers, inflammatory DNA damage including Progerin, p16, p21, IL‐1B, IL‐6, IL‐8, MCP1, γH2AX. Additionally, it restored NO production, enhanced proliferation, promoted angiogenesis, improved LDL uptake, mitochondrial ROS, membrane potential effectively. Our data suggest superior linear processes involved senescence.

Language: Английский

Citations

0
Multi-functional nanozyme-integrated astragalus polysaccharide hydrogel for targeted phased therapy in diabetic wound healing DOI
Xiaoru Zhang, S. X. Wen, Qin Liu

et al.

Nano Today, Journal Year: 2025, Volume and Issue: 62, P. 102739 - 102739

Published: April 1, 2025

Language: Английский

Citations

0
Repurposing of activating transcription factor 3 (ATF3) activator molecules with potential wound-healing effects DOI
Yolanda M. Jacobo-Delgado, Juan Valentin Trujillo‐Paez, Alan Santos‐Mena

et al.

Injury, Journal Year: 2025, Volume and Issue: 56(6), P. 112314 - 112314

Published: April 6, 2025

Language: Английский

Citations

0
Biomaterial scaffold-based gene delivery for the repair of complex wounds: Challenges, progress, and future perspectives DOI Creative Commons
Juan Carlos Palomeque Chávez, Matthew McGrath, Cathal J. Kearney

et al.

Published: April 1, 2025

Language: Английский

Citations

0
The Treatment of Large Bone Defects with Atrophic Nonunion by Bioactive Scaffolds Remodeling the Regeneration Microenvironment DOI

Zhuo Liang,

Fei Xue, J. Zhang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

Abstract Atrophic nonunion, a particularly challenging complication of diaphyseal bone fractures, arises from deteriorated regenerative microenvironment characterized by insufficient vascularization and pathological accumulation reactive oxygen species (ROS). To address this clinical challenge, dual‐function bioactive scaffolds is developed that simultaneously blocks disease progression promotes tissue regeneration. The design incorporates three key components: First, MnO 2 ‐Cu 2+ (MC) nanoparticles are synthesized to combat the through dual mechanisms ROS scavenging angiogenesis promotion. Second, circular morphogenetic protein (circBMP2) engineered for sustained BMP2 expression enhance osteogenic differentiation, with polyethylenimine‐mediated surface conjugation onto MC nanoparticles. Finally, these MC‐circBMP2 (MCB) complexes integrated into dopamine‐modified demineralized matrix create hierarchically structured platform. In vivo experiments showed promoted healing atrophic nonunion radial defects in rabbits improving angiogenesis, ROS, accelerating differentiation endogenous cells. above results indicate designed can precisely block pathology rapidly initiate regeneration damaged tissue, providing theoretical guidance refractory injuries.

Language: Английский

Citations

0
Mesenchymal Stem Cell-Derived Exosomes Hold Promise in the Treatment of Diabetic Foot Ulcers DOI Creative Commons
Hui Wang,

Sensen Wu,

Xinyu Bai

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 5837 - 5857

Published: May 1, 2025

Diabetic foot ulcers (DFU) represent one of the most common side effects diabetes, significantly impacting patients' quality life and imposing considerable financial burdens on families society at large. Despite advancements in therapies targeting lower limb revascularization various medications dressings, outcomes for patients with severe lesions remain limited. A recent breakthrough DFU treatment stems from development mesenchymal stem cells (MSCs). MSCs have shown promising results treating diseases skin wounds due to their ability multidirectional differentiation immunomodulation. Recent studies highlight that primarily repair tissue through paracrine activities, exosomes playing a crucial role as main biologically active components. These transport proteins, mRNA, DNA, other substances, facilitating immunomodulation, antioxidant effects, angiogenesis promotion, endothelial cell migration proliferation, collagen remodeling. Mesenchymal cell-derived (MSC-Exo) not only deliver comparable therapeutic but also mitigate adverse reactions like immune rejection associated transplantation. This article provides an overview pathophysiology explores mechanisms research progress MSC-Exo therapy.

Language: Английский

Citations

0