Application of Saccharide Cryoprotectants in the Freezing or Lyophilization Process of Lipid Nanoparticles Encapsulating Gene Drugs for Regenerative Medicine DOI Creative Commons

Wanqi Li,

Ting Wang, Jin Chen

et al.

Regenerative medicine and dentistry., Journal Year: 2024, Volume and Issue: unknown, P. 3 - 3

Published: Dec. 20, 2024

Article Application of Saccharide Cryoprotectants in the Freezing or Lyophilization Process Lipid Nanoparticles Encapsulating Gene Drugs for Regenerative Medicine Wanqi Li 1,†, Ting Wang Jianyang Chen 1, Minmei Guo Ling 1 and Akon Higuchi 1,2,3,* State Key Laboratory Ophthalmology, Optometry Visual Science, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, 325027, China 2 Department Chemical Materials Engineering, National Central 300, Jhongda RD., Jhongli District, Taoyuan 32001, Taiwan 3 R&D Center Membrane Technology, Chung Yuan Christian Chungli 32023, * Correspondence: [email protected] [email protected] † These authors contributed equally to this work. Received: 14 November 2024; Revised: 15 December Accepted: 16 Published: 20 2024 Abstract: nanoparticles (LNPs) have emerged as highly efficient drug delivery systems gene therapy regenerative medicine demonstrated great potential recent years. Notably, LNPs encapsulating mRNA vaccines achieved remarkable success combating COVID-19 epidemic. However, encounter issues physical chemical instability need be stored transported under harsh conditions. technology, which is commonly used increase stability nanomedicines, has been increasingly applied stabilize mRNA-LNPs. Appropriate cryoprotectants, such saccharides, glycerin, dimethyl sulfoxide (DMSO), added mRNA-LNPs during freezing lyophilization process effectively preserve properties mRNA-LNPs, ensuring their stability. Saccharides (i.e., sucrose, trehalose, maltose) are most widely cryoprotectants protect integrity This because saccharides relatively safe molecules compared with other cells animals. different varying levels protective effects on mRNA-LNP formulations, optimal saccharide concentration varies depending specific mRNA-LNP. article reviews application mechanisms saccharide-based LNP-delivered therapies medicines, offering guidance selecting appropriate drugs processes.

Language: Английский

A platform method for simultaneous quantification of lipid and nucleic acid components in lipid nanoparticles DOI
Wan-Chih Su,

Raymond Lieu,

Yige Fu

et al.

Journal of Chromatography A, Journal Year: 2025, Volume and Issue: 1746, P. 465788 - 465788

Published: Feb. 17, 2025

Language: Английский

Citations

1
Influence of salt solution on the physicochemical properties and in vitro/ in vivo expression of mRNA/LNP DOI Creative Commons
Siyuan Tang, Lei Huang, Jiahao Ge

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 19, 2025

Lipid nanoparticles (LNPs) have revolutionized nucleic acid delivery, enabling significant advances in mRNA-based therapeutics. While extensive research has focused on lipid composition, the impact of preparation solutions LNP performance remains underexplored. This study systematically investigated effects pH, salt type, and concentration across key solutions—mRNA aqueous, dilution, exchange, storage solutions—on physicochemical properties, stability, expression efficiency SM102-based mRNA/LNPs. Findings revealed that pH mRNA aqueous solution was critical, with a 4 optimizing encapsulation (EE) cellular expression. The exchange solution's significantly influenced biodistribution, particularly liver-specific following intravenous intramuscular administration. Sucrose identified as essential for freeze-thaw 300 mM minimizing aggregation leakage. Furthermore, were shown to influence structural integrity LNPs, impacting their vivo vitro performance. These insights highlight importance conditions formulations clinical applications, offering foundation enhanced therapeutic design delivery.

Language: Английский

Citations

0
Lipid nanoparticle (LNP) mediated mRNA delivery in neurodegenerative diseases DOI

Seyedeh Ghazal Moosavi,

Niloufar Rahiman, Mahmoud Reza Jaafari

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113641 - 113641

Published: March 1, 2025

Language: Английский

Citations

0
Recent Advances in Drying and Development of Solid Formulations for Stable mRNA and siRNA Lipid Nanoparticles DOI

Kinnari Santosh Arte,

Manlin Chen,

Chanakya Patil

et al.

Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

3
Formulation screening of lyophilized mRNA-lipid nanoparticles DOI Creative Commons
Anna Ruppl,

Denis Kiesewetter,

Monika Koell-Weber

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 125272 - 125272

Published: Jan. 1, 2025

Language: Английский

Citations

0
Cancer Nanovaccines: Mechanisms, Design Principles, and Clinical Translation DOI Creative Commons

Gabriel de Camargo Zaccariotto,

Maria Julia Bistaffa, Angelica Maria Mazuera Zapata

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Cancer immunotherapy has transformed the landscape of oncological treatment by employing various strategies to teach immune system eliminate tumors. Among these, cancer nanovaccines are an emerging strategy that utilizes nanotechnology enhance activation in response tumor antigens. This review addresses principles behind different technologies this field aimed at generating a robust and effective response. The diversity adopted for design is discussed, including types active agents, nanocarriers, their functionalizations, incorporation adjuvants. Furthermore, optimize nanoparticle formulations antigen presentation, target cells, organs promote strong durable antitumor responses explored. Finally, we analyze current state clinical application, highlighting ongoing trials future potential nanovaccines. insights presented aim guide research development efforts field, contributing advancement more targeted fight against cancer.

Language: Английский

Citations

0
Application of Saccharide Cryoprotectants in the Freezing or Lyophilization Process of Lipid Nanoparticles Encapsulating Gene Drugs for Regenerative Medicine DOI Creative Commons

Wanqi Li,

Ting Wang, Jin Chen

et al.

Regenerative medicine and dentistry., Journal Year: 2024, Volume and Issue: unknown, P. 3 - 3

Published: Dec. 20, 2024

Article Application of Saccharide Cryoprotectants in the Freezing or Lyophilization Process Lipid Nanoparticles Encapsulating Gene Drugs for Regenerative Medicine Wanqi Li 1,†, Ting Wang Jianyang Chen 1, Minmei Guo Ling 1 and Akon Higuchi 1,2,3,* State Key Laboratory Ophthalmology, Optometry Visual Science, Eye Hospital, Wenzhou Medical University, No. 270, Xueyuan Road, 325027, China 2 Department Chemical Materials Engineering, National Central 300, Jhongda RD., Jhongli District, Taoyuan 32001, Taiwan 3 R&D Center Membrane Technology, Chung Yuan Christian Chungli 32023, * Correspondence: [email protected] [email protected] † These authors contributed equally to this work. Received: 14 November 2024; Revised: 15 December Accepted: 16 Published: 20 2024 Abstract: nanoparticles (LNPs) have emerged as highly efficient drug delivery systems gene therapy regenerative medicine demonstrated great potential recent years. Notably, LNPs encapsulating mRNA vaccines achieved remarkable success combating COVID-19 epidemic. However, encounter issues physical chemical instability need be stored transported under harsh conditions. technology, which is commonly used increase stability nanomedicines, has been increasingly applied stabilize mRNA-LNPs. Appropriate cryoprotectants, such saccharides, glycerin, dimethyl sulfoxide (DMSO), added mRNA-LNPs during freezing lyophilization process effectively preserve properties mRNA-LNPs, ensuring their stability. Saccharides (i.e., sucrose, trehalose, maltose) are most widely cryoprotectants protect integrity This because saccharides relatively safe molecules compared with other cells animals. different varying levels protective effects on mRNA-LNP formulations, optimal saccharide concentration varies depending specific mRNA-LNP. article reviews application mechanisms saccharide-based LNP-delivered therapies medicines, offering guidance selecting appropriate drugs processes.

Language: Английский

Citations

0