Nano Today, Journal Year: 2025, Volume and Issue: 64, P. 102817 - 102817
Published: May 28, 2025
Language: Английский
Science Advances, Journal Year: 2025, Volume and Issue: 11(9)
Published: Feb. 26, 2025
Naturally occurring extracellular vesicles (EVs) and synthetic nanoparticles like liposomes have revolutionized precision diagnostics medicine. EVs excel in biocompatibility cell targeting, while offer enhanced drug loading capacity scalability. The clinical translation of is hindered by challenges including low yield heterogeneity, whereas face rapid immune clearance limited targeting efficiency. To bridge these gaps, biomimetic (SVs) emerged as innovative platforms, combining the advantageous properties liposomes. This review emphasizes critical aspects EV biology, such mechanisms EV-cell interaction source-dependent functionalities modulation, tissue regeneration, informing SV engineering. We reviewed a broad array SVs, with focus on lipid bilayered functionalized proteins. These include cell-derived nanovesicles, protein-functionalized liposomes, hybrid vesicles. By addressing current highlighting opportunities, this aims to advance SVs for transformative biomedical applications.
Language: Английский
Citations
3
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: Feb. 27, 2025
Androgenetic alopecia (AGA), a chronic and progressive disease, significantly impacts the patients' social, emotional, mental well-being. Current treatment for AGA are mainly limited by drug side effects stratum corneum (SC) barrier of scalp. To address these issues, we developed microneedle (MN) system loaded with black phosphorus nanosheets (BP) encapsulating baicalin (BA), natural ingredient, effective AGA. We first fabricated BP-BA based on BP properties high loading capacity excellent photothermal conversion efficiency. Upon 635 nm laser irradiation, demonstrated efficient to mild thermal ~ 42 °C. This effect controlled BA's stimuli-responsive release, enhanced cellular uptake, effectively modulated gene expression in dihydrotestosterone-treated human dermal papilla cells, downregulating negative regulators such as SRD5A2, AR, DKK1, TGFB1, while upregulating positive like CTNNBIP1 VEGFA. Furthermore, encapsulated MN fabricating BP-BA@MNs overcome SC barrier. Compared without irradiation penetration into subcutaneous area accumulation at follicular site. Importantly, synergistic efficacy against testosterone-induced vivo through combining BA chemotherapy, BP-mediated therapy, delivery, well good biocompatibility biosafety, underlying mechanism was elucidated terms microenvironment reconstruction. is promising approach targeted providing multifunctional strategy addressing clinical needs anti-AGA.
Language: Английский
Citations
2
Expert Opinion on Drug Delivery, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 22, 2025
Introduction Androgenic alopecia is a multifactorial disease with high incidence and great psychological burden on patients. The current FDA-approved treatment topical minoxidil or oral finasteride. However, both present significant limitations. While the systemic absorption of finasteride causes serious sexual side effects, minoxidil's low solubility imposes challenge in obtaining non-irritative effective formulation. One way to solve such limitations by using nanocarriers targeting drug delivery hair follicles upon application.
Language: Английский
Citations
1
Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)
Published: March 31, 2025
Mesenchymal stem cell exosomes are the most extensively researched type of Extracellular vesicles (EVs) that offer novel avenues for hair regeneration. However, their use in field regeneration was limited by poor production exosomes. It has been discovered intracellular (IVs), which produced at a higher rate than exosomes, play comparable biological purpose. As result, we developed HTMI-MN, microneedle uses tremella, Tremella polysaccharide (TPS), and hyaluronic acid (HA) as matrix materials. magnetic (Mag-IVs), work concert to treat androgenic alopecia (AGA) encourage growth. After characterization Mag-IVs, evaluated effect on angiogenesis scratch assay, Western Blot ELISA assay. In addition, tested protective Human papillary cells (HHDPCs) CCK-8 method, flow cytometry. Finally, effects tremella M1/M2 polarization macrophages were detected fluorescence staining, AGA model established vivo DHT, treatment given injection. Our study found Mag-IVs have greater power promote protect HHDPCs from apoptosis compared other vesicles. Besides, can make transformation anti-inflammatory phenotype. Taken together, experiments showed faster HTMI-MN-treated mice. These results indicate synergistically improve microenvironment, promising future treatment.
Language: Английский
Citations
1
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: April 8, 2025
The critical role of the immune system in brain function and dysfunction is well recognized, yet development therapies for psychiatric diseases has been slow due to concerns about iatrogenic deficiencies. These are emphasized by lack objective diagnostic tools psychiatry. A promise resolve this conundrum lies exploitation extracellular vesicles (EVs) that physiologically produced or can be synthetized. EVs regulate recipient cell functions offer potential EVs-based therapies. Intranasal administration enables targeting specific regions functions, thereby facilitating design precise treatments diseases. such requires navigating four dynamically interacting networks: neuronal, glial, immune, EVs. networks profoundly influenced fluid distribution. They crucial homeostasis, cellular intercellular communication. Fluid abnormalities, like edema altered cerebrospinal (CSF) dynamics, disrupt these networks, negatively impacting health. deeper understanding above-mentioned vital creating biomarker panels identify distinct patient subsets with similar neuro-behavioral symptoms. Testing functional pathways biomarkers could lead new therapeutic tools. Regulatory approval will depend on robust preclinical data reflecting progress interdisciplinary areas, which pave way innovative treatments. Highly collaborative teams needed achieve ambitious goals.
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 314, P. 144460 - 144460
Published: May 20, 2025
Language: Английский
Citations
0
BioNanoScience, Journal Year: 2025, Volume and Issue: 15(2)
Published: April 3, 2025
Language: Английский
Citations
0
Nano Today, Journal Year: 2025, Volume and Issue: 64, P. 102817 - 102817
Published: May 28, 2025
Language: Английский
Citations
0