Peptide-Modified Mesoporous Silica Nanoparticles for the Coordinated Regulation of Macrophage Polarization and Pyroptosis in the Treatment of Implant-Related Infections

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101629 - 101629

Published: March 4, 2025

Implant-related infections (IRIs) present a significant challenge in clinical treatment because of the formation biofilms. The complex architecture biofilms not only impedes antibiotic penetration, fostering evolution multidrug resistance bacteria under minimal selective pressure but also suppresses antimicrobial activity macrophages and induces their pyroptosis large quantities. This excessive impairs collective immune function macrophages, enabling pathogens to evade system clearance rendering infection difficult eradicate. Existing strategies often necessitate extensive surgical debridement, which causes harm patients' physiological health quality life results limited therapeutic outcomes. To address these challenges, this study developed mesoporous silica nanoparticle (MRL) modified with RGD (Arginine-Glycine-Aspartic acid) tripeptide loaded peptide LL-37. LL-37 released from MRL can directly disrupt bacterial cell membranes, preventing developing through conventional mutation mechanisms, enhance by modulating macrophage polarization toward M1 phenotype. However, may induce exacerbate within Therefore, we nanoparticles increase viability reduce number deaths, thereby alleviating immunosuppression caused pyroptosis. In vitro vivo experiments demonstrated that MRL, while preserving immunomodulatory LL-37, significantly reduced protected macrophages. Thus, fine-tuned regulation response was achieved, providing new insights for IRIs.

Language: Английский

Harnessing the human gut microbiota: an emerging frontier in combatting multidrug-resistant bacteria

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 17, 2025

Antimicrobial resistance (AMR) has become a major and escalating global health threat, undermining the effectiveness of current antibiotic antimicrobial therapies. The rise multidrug-resistant bacteria led to increasingly difficult-to-treat infections, resulting in higher morbidity, mortality, healthcare costs. Tackling this crisis requires development novel agents, optimization therapeutic strategies, initiatives infection surveillance control. Recent studies highlight crucial role human gut microbiota defending against AMR pathogens. A balanced protects body through mechanisms such as colonization resistance, positioning it key ally fight AMR. In contrast, dysbiosis disrupts defense, thereby facilitating persistence, colonization, dissemination resistant This review will explore how influence drug-resistant bacterial its involvement various types AMR-related potential for microbiota-targeted therapies, fecal transplantation, prebiotics, probiotics, phage therapy. Elucidating interactions between pathogens provide critical insights developing strategies prevent treat infections. While previous reviews have focused on general impact health, specifically look at latest research evolution spread AMR, highlighting strategies.

Language: Английский

Virulence arresting drugs discovery by strategies targeting bacterial virulence: mainly focusing on quorum-sensing interference and biofilm inhibition

Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101310 - 101310

Published: April 1, 2025

Language: Английский