A stretchable and biomimetic polyurethane membrane for lung alveolar in vitro modelling

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 25, 2025

Abstract The lung alveolus constitutes a morphologically and mechanistically complex tissue that is constantly subjected to cyclic tension exhibits unique elastic properties. Available materials used mimic alveolar often lack biomimicry the mechanical properties required for tension. Here, we report fully synthetic fibrous polyurethane scaffold approximates stiffness, under breathing simulations supports long-term culture of epithelial-like cells. Using electrospinning membrane tuneable thickness, set fibre diameter small pore size prepared. When uniaxial material retains its elasticity at both low high frequency mimicking human mouse breathing. Thanks size, cells can be cultured on apical surface forming an epithelial monolayer. This monolayer maintained long term (at least 15 days) in air–liquid interface. In latter conditions, differentiate exhibit expression surfactant protein A, constituent layer plays key role physiology. Owing lung-mimicking characteristics, electrospun holds potential adapted models.

Language: Английский

---

Fabrication Strategies for Bioinspired and Functional Lung‐on‐Chips

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(46)

Published: Aug. 16, 2024

Abstract Lung‐on‐chips (LoCs) are advanced microsystems designed to culture pulmonary cells under physiologically relevant conditions, including air–liquid interface, cell stretch, and shear stress. As the most promising preclinical models, LoCs aimed reduce ultimately replace conventional ineffective animal studies. Biomimetic functional lead more translational studies that effectively address unmet needs across therapeutic areas. A variety of cell‐ scaffold‐based techniques employed establish biomimetic models closely resemble their in vivo counterparts, which is a challenging yet critical aspect LoCs. Herein, challenges encountered discussed, future perspectives toward higher biomimicry using biofabrication approaches explored.

Language: Английский

---

A stretchable and biomimetic polyurethane membrane for lung alveolar in vitro modelling

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

ABSTRACT The lung alveolus constitutes a morphologically and mechanistically complex tissue that is constantly subjected to cyclic tension exhibits unique elastic properties. Available materials used mimic alveolar often lack biomimicry the mechanical properties required for tension. Here, we report fully synthetic fibrous polyurethane scaffold approximates stiffness, under breathing simulations supports long-term culture of epithelial-like cells. Using electrospinning membrane tuneable thickness, set fibre diameter small pore size prepared. When uniaxial material retains its elasticity at both low high frequency mimicking human mouse breathing. Thanks size, cells can be cultured on apical surface forming an epithelial monolayer. This monolayer maintained long term (at least 15 days) in air-liquid interface. In latter conditions, differentiate exhibit expression surfactant protein A, constituent layer plays key role physiology. Owing lung-mimicking characteristics, electrospun holds potential adapted models.

Language: Английский

---

Bioinspirierte Lunge–auf–Chip als Alternative zu Tierversuchen

BIOspektrum, Journal Year: 2023, Volume and Issue: 29(7), P. 755 - 757

Published: Nov. 1, 2023

Abstract Chronic respiratory diseases are one of the most common causes death globally. Biomimetic lung-on-chip (Lung Chip) technologies, as powerful preclinical in vitro models, have been developed to mimic physiological conditions human lungs. Lung Chips rapidly emerging promising and feasible alternative animal experimentation pharmaceutical research. Herein, I briefly present latest advancements Chips, well our contributions this field

Language: Английский

---