Chitosan-Based Intelligent Microneedles for Delivery of Amphotericin B Loaded Oleosomes: Antifungal Ocular Patch Targeting for Effective Against Fungal Keratitis Using Rabbit Model via TLR4/NLRP3 Pathway

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 5949 - 5981

Published: May 1, 2025

Language: Английский

Isoform-Specific vs Isoform-Universal Drug Targeting: A New Targeting Paradigm Illustrated by New Anti-ICAM-1 Antibodies

Journal of drug targeting, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: Dec. 6, 2024

Drug targeting can be achieved by coupling drugs or their carriers to affinity molecules, mostly antibodies (Abs), which recognize specific protein targets. However, most proteins are not expressed in an exclusive configuration but as various isoforms. Hence, selected molecules may fail target with enough efficiency clinical trials, is overlooked. We illustrate this ICAM-1, a cell-surface overexpressed many pathologies. Most ICAM-1 studies used Ab R6.5, binds domain 2 (D2). Yet, literature and our data show that D2 frequently absent among thus produced battery of five new Abs (B4, B6, B11, C12, G2) tested ability both full-length -D2 ICAM-1. In solution, all recognized forms (from 5.3x10

Language: Английский

Presence of ICAM-1 Isoforms in Human Cells Impacts the Selection of Antibodies for Nanocarrier Targeting

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 106582 - 106582

Published: Dec. 1, 2024

Language: Английский