Electrospun Nanofiber-Scaffold-Loaded Levocetirizine Dihydrochloride Cerosomes for Combined Management of Atopic Dermatitis and Methicillin-Resistant Staphylococcus Aureus (MRSA) Skin Infection: In Vitro and In Vivo Studies DOI Creative Commons
Rofida Albash, Samer Khalid Ali, Rehab Abdelmonem

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(5), P. 633 - 633

Published: April 27, 2025

Objectives: In this study, we aimed to incorporate levocetirizine dihydrochloride (LVC) into electrospun nanovesicle-in-nanofiber (NF) scaffolds for combined management of atopic dermatitis and methicillin-resistant Staphylococcus Aureus skin infection, sustain LVC release continuous improvement. Methods: Firstly, was encapsulated in cerosomes (CERs) by employing a thin-film hydration approach using 21.31 factorial design. CERs were assessed calculating entrapment efficiency (EE%), particle size (PS) polydispersity index (PDI). addition, the optimized further subjected stability evaluation. After that, incorporated polyurethane nanofibers (NFs) coaxial electrospinning technique. An vitro assay used calculate amount released from LVC-NFs CERs-NFs. For morphological assessment NFs, CERs-NFs transmission electron microscopy, scanning confocal laser microscopy. Atomic force microscopy utilized evaluate roughness both NFs. The optimum formulation vivo study. Results: exhibited an EE% 65.03 ± 1.07%, PS 680.00 39.50 nm, PDI 0.51 0.04. sustained manner Further, dermatokinetic study confirmed that infiltration LVC, compared with other groups. Finally, safety showed all formulations safe when topically applied rat skin. Conclusions: conclusion, AD MRSA infections may be cured nanofiber-scaffold-loaded CERs, which can thus regarded as promising system.

Language: Английский

Electrospun Nanofiber-Scaffold-Loaded Levocetirizine Dihydrochloride Cerosomes for Combined Management of Atopic Dermatitis and Methicillin-Resistant Staphylococcus Aureus (MRSA) Skin Infection: In Vitro and In Vivo Studies DOI Creative Commons
Rofida Albash, Samer Khalid Ali, Rehab Abdelmonem

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(5), P. 633 - 633

Published: April 27, 2025

Objectives: In this study, we aimed to incorporate levocetirizine dihydrochloride (LVC) into electrospun nanovesicle-in-nanofiber (NF) scaffolds for combined management of atopic dermatitis and methicillin-resistant Staphylococcus Aureus skin infection, sustain LVC release continuous improvement. Methods: Firstly, was encapsulated in cerosomes (CERs) by employing a thin-film hydration approach using 21.31 factorial design. CERs were assessed calculating entrapment efficiency (EE%), particle size (PS) polydispersity index (PDI). addition, the optimized further subjected stability evaluation. After that, incorporated polyurethane nanofibers (NFs) coaxial electrospinning technique. An vitro assay used calculate amount released from LVC-NFs CERs-NFs. For morphological assessment NFs, CERs-NFs transmission electron microscopy, scanning confocal laser microscopy. Atomic force microscopy utilized evaluate roughness both NFs. The optimum formulation vivo study. Results: exhibited an EE% 65.03 ± 1.07%, PS 680.00 39.50 nm, PDI 0.51 0.04. sustained manner Further, dermatokinetic study confirmed that infiltration LVC, compared with other groups. Finally, safety showed all formulations safe when topically applied rat skin. Conclusions: conclusion, AD MRSA infections may be cured nanofiber-scaffold-loaded CERs, which can thus regarded as promising system.

Language: Английский

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