Tunable PhenoCycler Imaging of the Murine Pre-Clinical Tumour Microenvironments DOI Creative Commons
Madelyn Jean Abraham, Christophe Gonçalves, Paige McCallum

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 22, 2023

Abstract The tumour microenvironment (TME) consists of tumour-supportive immune cells, endothelial and fibroblasts. PhenoCycler, a high-plex single cell imaging platform, is used to characterize the complexity TME. Here, we PhenoCycler spatially resolve TME 8 routinely employed pre-clinical models lymphoma, breast cancer, melanoma. Our data reveal distinct TMEs in different cancer that were imaged, show cell-cell contacts differ depending on type examined. For instance, found infiltration murine model melanoma altered cellular organization melanomas become resistant αPD-1 therapy, with depletions number interactions. Furthermore, provide detailed pipelines for conjugation antibodies are optimized staining FFPE tissues specifically, alongside open-source analysis procedures. Overall, this valuable resource study seamlessly adaptable any field research involving models.

Language: Английский

Tunable PhenoCycler imaging of the murine pre-clinical tumour microenvironments DOI Creative Commons
Madelyn Jean Abraham, Christophe Gonçalves, Paige A. McCallum

et al.

Cell & Bioscience, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 4, 2024

The tumour microenvironment (TME) consists of tumour-supportive immune cells, endothelial and fibroblasts. PhenoCycler, a high-plex single cell spatial biology imaging platform, is used to characterize the complexity TME. Researchers worldwide harvest bank tissues from mouse models which are employed model plethora human disease. With explosion interest in biology, these panoplies archival provide valuable resource answer new questions. Here, we describe our protocols for developing tunable PhenoCycler multiplexed panels open-source data analysis pipeline. Using protocols, spatially resolve TME 8 routinely pre-clinical lymphoma, breast cancer, melanoma preserved as FFPE.

Language: Английский

Citations

3
Abnormal metabolism in melanocytes participates in the activation of dendritic cell in halo nevus DOI

Ling Jiang,

Yibo Hu,

Yushan Zhang

et al.

Clinical Immunology, Journal Year: 2024, Volume and Issue: 265, P. 110300 - 110300

Published: June 29, 2024

Language: Английский

Citations

2
Nevi DOI

Asja Prohic

Published: Jan. 1, 2024

Citations

0
Tunable PhenoCycler Imaging of the Murine Pre-Clinical Tumour Microenvironments DOI Creative Commons
Madelyn Jean Abraham, Christophe Gonçalves, Paige McCallum

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 22, 2023

Abstract The tumour microenvironment (TME) consists of tumour-supportive immune cells, endothelial and fibroblasts. PhenoCycler, a high-plex single cell imaging platform, is used to characterize the complexity TME. Here, we PhenoCycler spatially resolve TME 8 routinely employed pre-clinical models lymphoma, breast cancer, melanoma. Our data reveal distinct TMEs in different cancer that were imaged, show cell-cell contacts differ depending on type examined. For instance, found infiltration murine model melanoma altered cellular organization melanomas become resistant αPD-1 therapy, with depletions number interactions. Furthermore, provide detailed pipelines for conjugation antibodies are optimized staining FFPE tissues specifically, alongside open-source analysis procedures. Overall, this valuable resource study seamlessly adaptable any field research involving models.

Language: Английский

Citations

0