Obtaining mutant Chlorella vulgaris strains with excellent self-flocculation properties and high wastewater treatment efficiency via atmospheric and room temperature plasma technique DOI

Guixia Ma,

Qianya Meng,

Ruimin Mu

et al.

Journal of Water Process Engineering, Journal Year: 2023, Volume and Issue: 53, P. 103644 - 103644

Published: March 22, 2023

Language: Английский

Comprehensive analysis of codon bias in 13 Ganoderma mitochondrial genomes DOI Creative Commons
Peng Wu,

Wenqi Xiao,

Yingyong Luo

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: May 4, 2023

Codon usage bias is a prevalent phenomenon observed across various species and genes. However, the specific attributes of codon in mitochondrial genome Ganoderma remain unknown.In this study, we investigated 12 core protein-coding genes (PCGs) 9 species, including 13 strains.The codons all strains showed preference for ending A/T. Additionally, correlations between base composition adaptation index (CAI), (CBI) frequency optimal (FOP) were identified, demonstrating impact on bias. Various indicators found to vary or within strains, GC3s, CAI, CBI, FOP. The results also revealed that PCGs have an average effective number (ENC) lower than 35, indicating strong toward certain codons. Evidence from neutrality plot PR2-bias analysis indicates natural selection major factor affecting Ganoderma. 11 22 (ΔRSCU>0.08 RSCU>1) identified with GCA, AUC, UUC being most widely used By analyzing combined sequences relative synonymous (RSCU) values, genetic relationships determined, variations them. Nevertheless, RSCU-based illustrated intra- interspecies species.This study deepens our insight into characteristics, genetics, evolution important fungal group.

Language: Английский

Citations

14
Application of biophosphates in geo-environmental engineering: An overview DOI Creative Commons
Jiangshan Li, Lijun Han, Qiang Xue

et al.

Biogeotechnics, Journal Year: 2025, Volume and Issue: unknown, P. 100165 - 100165

Published: Jan. 1, 2025

Language: Английский

Citations

0
Three local plants adapt to ecological restoration of abandoned lead-zinc mines through assembly of rhizosphere bacterial communities DOI Creative Commons
Wei Gao, Shuyi Chen,

Xin Xiao Yu

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 10, 2025

Introduction The plant restoration and ecological of lead-zinc mines are very important. Methods In this study, we used three local plants to carry out abandoned lead–zinc mining areas detected the adaptive mechanisms soil bacterial diversity function during through 16S rRNA sequencing. Results results revealed that significantly reduced diversity, including Shannon, Simpson, observed species indices, whereas planting restored microbial a certain extent, leading increases in Shannon index Observed indices. Mining activities abundances RB41 Bryobacter bulk compared with those nonmining areas, increased rhizosphere areas. Following types ecologically plants, community structure partially recovered. addition, different have been found functions process, iron complex transport system-permitting proteins ATP binding cassettes. Discussion This study confirms for first time adapt remediation process by non-randomly assembling communities functions, providing reference screening resources microbe joint bioremediation strategies mines.

Language: Английский

Citations

0
Improvement of Esterifying Power of Isolated Bacillus velezensis from Daqu by Atmospheric Pressure and Room Temperature Plasma Mutagenesis DOI Creative Commons
Chuan Song, Tongwei Guan, Zhuang Xiong

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 800 - 800

Published: Feb. 26, 2025

Strong-flavor Baijiu, a widely popular distilled spirit in China, derives its characteristic aroma and quality largely from ethyl hexanoate, key flavor compound. The concentration of influenced by precursor hexanoic acid, is critical defining the style this Baijiu variety. In study, atmospheric room temperature plasma (ARTP) mutagenesis technology was applied to strains isolated Daqu enhance their acid ester production capabilities. A acid-producing strain, identified as Bacillus velezensis WY4 through morphological, physiological, biochemical, molecular analyses, used starting strain. Following 90 s ARTP exposure, mutant WY4-3, successfully developed, achieving balance between high mutation diversity moderate lethality. WY4-3 exhibited robust growth across pH range 4.2 5.0 demonstrated ethanol tolerance. After five days fermentation, produced 0.36 g/L total 0.528 ester, surpassing wild-type Enzymatic activity assays revealed significant enhancements amylase (9.13%), saccharifying enzyme (101.72%), esterification (573.71%) activities WY4-3. Validation multiple artificial systems further confirmed superior capacity These findings enrich microbial germplasm resources for brewing provide solid foundation strain selection genetic improvement processes. This study highlights potential optimizing microorganisms improving consistency Baijiu.

Language: Английский

Citations

0
Physical, Thermal, Chemical and Biological approaches for Plastics degradation - A review DOI Creative Commons

Seetharam Pondala,

Sathish Mohan Botsa

Cleaner Chemical Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 100162 - 100162

Published: March 1, 2025

Language: Английский

Citations

0
Advancing VB12 Production: Insights into Enhancing VB12 Titer in Ensifer adhaerens Casida A through ARTP Mutagenesis and Multiomics Analysis DOI
Wang Qi, Yongheng Liu, Tengteng Zhu

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Ensifer adhaerens, a microorganism recognized for its capacity to synthesize vitamin B12 (VB12), has garnered significant attention in recent years. Nonetheless, practical application been limited by low production yields. Atmospheric and room-temperature plasma (ARTP) mutagenesis was utilized improve VB12 examine the associated mechanisms. Three high-yielding mutant strains─BCA-24, BCB-14, BCC-27─were isolated through multiple rounds of mutagenesis. The titer highly productive strain, BCA-24, rose significantly from 65.64 mg/L 104.54 mg/L. Genome resequencing identified 14 mutated genes, which seven (atpA, gntR, fusA, cobQ, ribD, cirA, UP) were functionally validated overexpression wild-type strains found positively influence biosynthesis. Notably, coexpression cobQ UP genes strain BCA-24 resulted 163.68 Transcriptomic analysis indicated that critical pathways related energy metabolism, S-adenosylmethionine (SAM), 5-aminolevulinic acid (5-ALA), riboflavin synthesis upregulated relative wild type. A multiomics approach clarified mechanisms these mutations increase production, including enhanced transcription translation, optimized supply, improved product efflux. identification novel candidate have not previously studied, provides valuable resources future genetic engineering aimed at enhancing efficiency. This study offers improvements microbial while also delivering essential insights into metabolic regulation this important biosynthetic pathway.

Language: Английский

Citations

0
Addition of vegetable oil to enhance the biosynthesis of butenyl-spinosyn in a high production strain Saccharopolyspora pogona DOI Creative Commons

Zhouqin Xu,

Xinying Li, Chao Guo

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0319332 - e0319332

Published: March 26, 2025

Butenyl-spinosyn discovered from Saccharopolyspora pogona , is a broad-spectrum bioinsecticide. In order to further improve the production, fermentation medium of high-production strain Sa. ASAGF30A11 obtained by mutagenesis, was optimized adding different species and concentrations vegetable oil. our study, effect peanut oil on growth production proved monitoring curves, key gene transcription level content acyl-CoA. After 10 g/L oil, additional carbon sources redirected flux toward growth, inhibiting synthesis butenyl-spinosyn, while increasing biomass approximately 1.5-fold. However, when 1 it functions as surfactant, greatly promoting resulting in 1.52-fold increase production. The research provides promising strategy butenyl-spinosyn

Language: Английский

Citations

0
A Review of Chromium Behavior in Soil and its Remediation Techniques DOI
Jifei Wang, Xiangmei Li, Wei Wang

et al.

Water Air & Soil Pollution, Journal Year: 2024, Volume and Issue: 235(6)

Published: May 22, 2024

Language: Английский

Citations

3
Nitrogen removal characteristics and Cr(VI) tolerance mechanisms of heterotrophic nitrifying bacterium Pseudomonas putida strain LX1 DOI
Lei Yang,

Haoqi Lu,

Yu‐Chao Wang

et al.

Journal of Water Process Engineering, Journal Year: 2024, Volume and Issue: 64, P. 105647 - 105647

Published: June 19, 2024

Language: Английский

Citations

3
Research on the Effect of Natural Seawater in Domesticating Bacillus pasteurii and Reinforcing Calcareous Sand DOI Creative Commons
Ziyu Wang, Wenjing Chen,

Zhiyao Tong

et al.

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(4), P. 542 - 542

Published: March 25, 2024

Microbial-Induced Calcium Carbonate Precipitation (MICP) is an environmentally friendly, efficient, and sustainable new soil reinforcement technology. For this study, Bacillus pasteurii were domesticated cultured in a natural seawater environment with multiple gradients used for coral reef calcareous sand reinforcement, comparing the mineral composition of generated precipitates strength under different domestication gradient conditions. The results revealed that, while inhibits growth pasteurii, method allows bacteria to gradually adapt environment. Notably, their shape becomes thin long Furthermore, MICP mineralisation reaction rate faster and, increase gradient, increased. At same time, environment, small amount components addition CaCO3, such as MgxCay(CO3)z, Mg2+ content increased gradient. When curing effect it was found that can effectively enhance after multi-gradient increasing study provide ideas application technology environments construction South China Sea islands reefs.

Language: Английский

Citations

3