The Journal of Physical Chemistry B,
Journal Year:
2024,
Volume and Issue:
128(41), P. 10272 - 10285
Published: Oct. 8, 2024
Predicting
the
interfacial
properties
of
peptides
is
important
for
replacing
oil-derived
surfactants
in
cosmetics,
oil,
and
agricultural
applications.
This
work
validated
experimentally
estimations
surface
tension
at
critical
micelle
concentration
(STCMC)
six
performed
through
a
random
forest
(RF)
model
previous
contribution.
In
silico
tensions
were
obtained
system
decane–water,
dilational
experiments
applied
to
elucidate
foaming
potential.
The
RF
accurately
classified
into
high
low
potential
reduce
STCMC.
simulations
decane–water
interface
correctly
identified
with
high,
intermediate,
properties,
rheology
allowed
estimation
possible
three
produce
foams.
study
sets
basis
identifying
surface-active
peptides,
but
future
necessary
improve
correlation
between
foam
stabilization.
Topics in Catalysis,
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 18, 2024
Abstract
Lignocellulose
is
considered
to
be
the
most
abundant
and
sustainable
material
on
earth.
The
concept
of
lignocellulosic
biomass
conversion
into
value-added
chemicals
or
materials
gaining
in
importance
worldwide
as
a
means
replacing
conventional
petrochemical
resources
for
environmental
sustainability.
production
biofuels
such
bioethanol
from
consists
three
main
processes:
pretreatment,
enzymatic
saccharification,
fermentation.
As
lignocellulose
exhibits
highly
recalcitrant
structure,
effective
pretreatments
are
required
its
deconstruction,
making
carbohydrates
accessible
microbes
produce
valuable
bioproducts.
These
carbohydrate
polymers
(cellulose
hemicellulose)
then
transformed
free
monomeric
sugars
by
process
saccharification.
Saccharification,
especially
hydrolysis,
crucial
step
achieving
bioconversion.
Several
strategies
have
been
developed
diminishing
recalcitrance,
ultimately
improving
efficiency
product
conversion,
reducing
overall
costs.
Some
these
approaches
include
consolidated
bioprocessing,
bio-saccharification
(on
site),
well
simultaneous
saccharification
fermentation,
separate
hydrolysis
fermentation
(off
site).
This
review
provides
detailed
overview
current
on-site
off-site
highlights
key
factors
obtaining
bioproducts
feedstock
via
economically
feasible
bioconversion
processes.
Moreover,
optimization
various
industrially
important
biomasses
also
summarized.
Sustainability,
Journal Year:
2024,
Volume and Issue:
16(18), P. 7970 - 7970
Published: Sept. 12, 2024
Oil
spills
occur
during
different
operations
in
the
energy
sector,
such
as
crude
oil
transport,
tank
filling
and
cleaning,
fueling.
Such
are
one
of
major
causes
accumulation
derivatives
environment,
requiring
remediation
soil
marine
environments.
The
production
a
biosurfactant
by
Starmerella
bombicola
ATCC
222214
was
maximized
investigating
effect
carbon/nitrogen
sources
culture
conditions.
mineral
medium
selected
for
its
supplemented
with
2.0%
potato
peel
flour,
5.0%
waste
canola
frying
oil,
0.20%
urea.
conditions
were
200
rpm
shaking
speed,
fermentation
time
180
h,
4.0%
inoculum
size.
yield
isolated
7.72
g/L.
emulsification
rates
heavy
motor
65.55
95.00%,
respectively,
indicating
an
affinity
complex
hydrocarbons.
In
stability
tests
performed
at
pH
values,
temperatures,
NaCl
concentrations,
surface
tension
ranged
from
27.14
to
31.08
mN/m.
critical
micelle
concentration
2.0
g/L,
which
33.26
composed
6,6-dimethoxy-octanoic
acid
azelaic
acid,
it
exhibited
low
toxicity
Brassica
oleracea
Solanum
lycopersicum.
kinetic
test,
allowed
removal
82.30%,
96.65%,
98.25%
exhaust
sand,
silty
soil,
clay
while
static
test
packed
columns,
yields
66.62%,
63.03%,
58.45%,
respectively.
produced
this
study
is
promising
environmental
applications
sector.
PeerJ,
Journal Year:
2025,
Volume and Issue:
13, P. e18616 - e18616
Published: Jan. 31, 2025
Thermophilic
cellulases
can
play
a
crucial
part
in
the
efficient
breakdown
of
cellulose-a
major
component
lignocellulosic
plant
biomass,
however,
their
commercial
production
needs
simple
and
robust
biomanufacturing
biosystems.
In
this
study,
two
(β-glucosidase
endoglucanase)
were
heterologously
expressed
Escherichia
coli
under
chloroplast-derived
constitutive
promoter
expression-enhancing
terminator.
The
genes
encoding
sourced
from
thermophilic
bacterium
Thermotoga
maritima
to
exploit
industrially
needed
thermotolerance
potential.
codon-optimized
gene
sequences
synthesized
placed
tobacco
chloroplast
16S
rRNA
(Prrn),
along
with
5'
UTR
(untranslated
region)
10
phage
T7
(T7g10).
A
six-residue
long
histidine
tag
(His6-tag)
was
attached
N-terminus
for
protein
detection.
high-level
expression
β-glucosidase
endoglucanase
E.
recorded
Furthermore,
activity
assays
confirmed
that
recombinant
enzymes
maintained
at
elevated
temperatures.
Thermostability
analysis
showed
retained
even
after
being
non-native
host.
Where,
optimum
activities
90
°C
100
°C,
respectively.
Examination
3D
structures
T.
revealed
differential
ionic
interactions
contributing
high
degree
thermotolerance.
study
highlights
feasibility
producing
thermostable
versions
levels.
Our
finding
underscores
potential
approach
meet
industrial
demands
enzyme
employing
as
platform.