Optical Investigation of DNA Behaviour in Flow using Confocal Detection Incorporated with Fluorescence Correlation Spectroscopy (FCS) DOI Creative Commons
Fatma Doğan Güzel, Hamed Ghorbanpoor

Journal of Fluorescence, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Abstract Investigation of the behavior biopolymers in flow such as Deoxyribonucleic acid (DNA), is a critical challenge engineering, polymer, and life sciences. In this study, we studied rheological properties DNA characteristics real-time. The velocity measurement was carried out using confocal detection incorporated with Fluorescence Correlation Spectroscopy (FCS). Optical experiments provided an understanding diffusion- flow-dominated regimes for molecules treated microfluidic channels lab-on-chip devices general. We found that regime starts at rate 0.3 µl/min transitional falls into 0.02–0.3 rates. There are few examples different fragments such. It therefore believed to provide valuable insights subject dynamics DNA.

Language: Английский

Optical Investigation of DNA Behaviour in Flow using Confocal Detection Incorporated with Fluorescence Correlation Spectroscopy (FCS) DOI Creative Commons
Fatma Doğan Güzel, Hamed Ghorbanpoor

Journal of Fluorescence, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Abstract Investigation of the behavior biopolymers in flow such as Deoxyribonucleic acid (DNA), is a critical challenge engineering, polymer, and life sciences. In this study, we studied rheological properties DNA characteristics real-time. The velocity measurement was carried out using confocal detection incorporated with Fluorescence Correlation Spectroscopy (FCS). Optical experiments provided an understanding diffusion- flow-dominated regimes for molecules treated microfluidic channels lab-on-chip devices general. We found that regime starts at rate 0.3 µl/min transitional falls into 0.02–0.3 rates. There are few examples different fragments such. It therefore believed to provide valuable insights subject dynamics DNA.

Language: Английский

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