Frontiers in Microbiology,
Journal Year:
2023,
Volume and Issue:
14
Published: July 3, 2023
The
oil
sands
mining
operations
in
Alberta
have
produced
billions
of
m3
tailings
which
must
be
reclaimed
and
integrated
into
various
mine
closure
landforms,
including
terrestrial
landforms.
Microorganisms
play
a
central
role
nutrient
cycling
during
the
reclamation
disturbed
landscapes,
contributing
to
successful
vegetation
restoration
long-term
sustainability.
However,
microbial
community
succession
response
reconstructed
revegetated
remain
largely
unexplored.
This
study
aimed
monitor
structural
functional
responses
communities
subjected
different
capping
strategies
over
two
growing
seasons
(GS).
To
achieve
this,
column-based
greenhouse
experiment
was
conducted
investigate
that
were
capped
with
layer
(10
or
30
cm)
peat-mineral
mix
(PMM)
planted
either
upland
wetland
communities.
DNA
metabarcoding
analysis
bacterial
16S
rRNA
gene
fungal
ITS2
region
as
well
shotgun
metagenomics
used
asses
impact
treatments
on
taxonomy
functions,
respectively.
Results
showed
diversity
composition
changed
considerably
after
GS
compared
baseline
samples,
while
PMM
much
more
stable.
Likewise,
several
functions
significantly
enriched
GS.
Interestingly,
varied
depending
plant
community,
leading
higher
number
differentially
abundant
taxa
decrease
Shannon
evenness
treatment
but
not
treatment.
Moreover,
presence
increased
energy-related
metabolic
(carbon,
nitrogen,
sulfur),
these
depleted
by
Fungi
represented
small
proportion
tailings,
relative
abundance
time,
favored
growth
some
beneficial
taxa,
notably
root
endophyte
Serendipita,
both
columns.
results
suggest
selecting
right
combination
material
type
may
contribute
improve
below-ground
processes
sustain
harsh
environments
such
tailings.
Frontiers in Microbiology,
Journal Year:
2023,
Volume and Issue:
14
Published: June 2, 2023
Coastal
wetlands
(CW)
are
the
junction
of
terrestrial
and
marine
ecosystems
have
special
ecological
compositions
functions,
which
important
for
maintaining
biogeochemical
cycles.
Microorganisms
inhabiting
in
sediments
play
key
roles
material
cycle
CW.
Due
to
variable
environment
CW
fact
that
most
affected
by
human
activities
climate
change,
severely
degraded.
In-depth
understanding
community
structure,
function,
environmental
potential
microorganisms
is
essential
wetland
restoration
function
enhancement.
Therefore,
this
paper
summarizes
microbial
structure
its
influencing
factors,
discusses
change
patterns
functional
genes,
reveals
functions
microorganisms,
further
proposes
future
prospects
about
studies.
These
results
provide
some
references
promoting
application
cycling
pollution
remediation
Abstract
Metagenomic
strategy
serves
as
the
foundation
for
ecological
exploration
of
novel
bioresources
(e.g.,
industrial
enzymes
and
bioactive
molecules)
biohazards
pathogens
antibiotic
resistance
genes)
in
natural
engineered
microbial
systems
across
multiple
disciplines.
Recent
advancements
sequencing
technology
have
fostered
rapid
development
field
microbiome
research
where
an
increasing
number
studies
applied
both
illumina
short
reads
(SRs)
nanopore
long
(LRs)
their
metagenomic
workflow.
However,
given
high
complexity
environmental
data
set
bioinformatic
challenges
caused
by
unique
features
these
technologies,
integrating
SRs
LRs
is
not
straightforward
one
might
assume.
The
fast
renewal
existing
tools
growing
diversity
new
algorithms
make
access
to
this
even
more
difficult.
Therefore,
here
we
systematically
summarized
complete
workflow
from
DNA
extraction
processing
strategies
applying
nanopore‐integrated
metagenomics
investigation
microbiomes.
Overall,
review
aims
provide
a
timely
knowledge
framework
researchers
that
are
interested
or
struggling
with
integration
analysis.
discussions
presented
will
facilitate
improved
understanding
community
functionalities
assembly
natural,
engineered,
human
microbiomes,
benefiting
Environmental Microbiology,
Journal Year:
2022,
Volume and Issue:
24(12), P. 6200 - 6219
Published: Sept. 9, 2022
Abstract
The
rice
rhizosphere
microbiota
is
crucial
for
crop
yields
and
nutrient
use
efficiency.
However,
little
known
about
how
co‐occurrence
patterns,
keystone
taxa
functional
gene
assemblages
relate
to
soil
pH
in
the
soils.
Using
shotgun
metagenome
analysis,
microbiome
was
investigated
across
28
fields
east‐central
China.
At
higher
sites,
taxonomic
network
of
soils
more
complex
compact,
as
defined
by
average
degree,
graph
density
complexity.
Network
stability
greatest
at
medium
(6.5
<
7.5),
followed
high
(7.5
pH).
Keystone
were
abundant
correlated
significantly
with
key
ecosystem
functions.
Overall
genes
involved
C,
N,
P
S
cycling
a
relative
abundance
soils,
excepting
C
degradation
(e.g.
starch,
cellulose,
chitin
lignin
degradation).
Our
results
suggest
that
microbial
stable
pH,
possibly
indicating
increased
efficiency
cycling.
These
observations
may
indicate
routes
towards
efficient
management
understanding
potential
effects
acidification
on
system.
Frontiers in Plant Science,
Journal Year:
2021,
Volume and Issue:
12
Published: Dec. 20, 2021
The
shortage
of
land
resources
restricts
the
sustainable
development
agricultural
production.
Multiple
cropping
has
been
widely
used
in
Southern
China,
but
whether
continuous
planting
will
cause
a
decline
soil
quality
and
crop
yield
is
unclear.
To
test
multiple
could
increase
grain
yield,
we
investigated
farmlands
with
different
cultivation
years
(10-20
years,
20-40
>40
years).
Results
showed
that
tobacco-rice
rotation
significantly
increased
pH,
nitrogen
nutrient
content,
it
richness
bacterial
community.
farmland
highest
organic
carbon
(SOC),
ammonium
nitrogen,
there
no
significant
difference
diversity
structure
community
years.
molecular
ecological
network
indicated
stability
decreased
across
which
may
result
yields
system>
40
Acidobacteria
members
as
keystone
taxa
(Zi
≥
2.5
or
Pi
0.62)
appeared
farmlands,
abundance
was
found
SOC
suggesting
Gp4,
GP7,
GP12,
GP17
are
important
involved
cycle.
Therefore,
this
study,
systems
for
20
not
reduce
production
potential,
they
cannot
last
more
than
This
study
provides
insights
ensuring
enhancing
capacity.
