ACS ES&T Engineering,
Journal Year:
2024,
Volume and Issue:
4(9), P. 2177 - 2185
Published: Aug. 5, 2024
This
study
tested
polyhydroxyalkanoate
(PHA)
production
by
usingPseudomonas
putida
KT2440
in
a
5
L
lab-scale
bioreactor,
employing
an
A
cell
dry
mass
concentration
of
8.69
±
0.45
g/L
was
achieved
with
volatile
fatty
acid
(VFA)
removal
efficiency
81.75
3.08%.
Remarkably,
this
the
highest
PHA
fraction
(34.81
1.67%).
Analysis
revealed
that
poly(3-hydroxybutyrate)
(PHB)
contributes
83.6
to
86.1%
total
followed
poly(3-hydroxyhexanoate)
(PHHx)
(6.6–8.6%)
and
poly(3-hydroxy-2-methylvalerate)
(PH2MV)
(4.9–8.3%).
Thermogravimetric
analysis
showed
polymer
started
degrading
at
temperature
150
°C
maximum
weight
loss
occurring
approximately
270
°C.
Differential
scanning
calorimetry
two
peaks
corresponding
melting
polymer:
140.6,
154.87
suggests
resulting
blend
different
copolymers.
outcome
is
poised
advance
development
unified
methods
within
circular
bioeconomy,
enhancing
both
economic
environmental
advantages.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(12), P. 6110 - 6117
Published: Feb. 19, 2024
Establishing
a
platform
for
the
bioconversion
of
waste
resources
into
value-added
compounds
is
critical
achieving
sustainable
and
eco-friendly
economy.
Herein,
we
produced
polyhydroxyalkanoate
via
microbial
fermentation
using
cabbage
as
feedstock
metabolically
engineered
Escherichia
coli.
For
this,
hydrolysis
conditions
were
optimized
by
focusing
on
parameters
such
substrate
enzyme
concentrations
to
enhance
saccharification
efficiency.
The
phaABC
operon,
which
encodes
key
enzymes
responsible
biosynthesis
in
Ralstonia
eutropha
H16,
was
overexpressed
E.
Using
hydrolysate
feedstock,
this
coli
strain
could
produce
poly(3-hydroxybutyrate)
with
polymer
content
26.0
wt
%
dry
cell
weight.
Moreover,
malic
acid
significantly
enhanced
production;
addition
0.5
g/L
markedly
increased
59.9%.
This
study
demonstrates
potential
promising
raw
material
production
polyhydroxyalkanoate.
ACS Omega,
Journal Year:
2024,
Volume and Issue:
9(28), P. 30169 - 30182
Published: June 28, 2024
The
development
of
biodegradable
mulch
for
agricultural
applications
represents
a
sustainable
approach
to
reducing
plastic
pollution.
Poly(vinyl
alcohol)
(PVA)
is
one
the
nontoxic
and
polymers
that
can
be
used
as
mulching
film.
However,
major
drawback
PVA
its
moisture
sensitivity,
which
limits
applications.
In
this
study,
biocomposite
based
on
polyhydroxybutyrate
(PHB),
plasticized
with
triacetin,
was
developed
by
solvent
casting
method.
film
exhibited
good
mechanical
properties,
better
integrity,
reduced
transmittance,
light-blocking
prevent
weed
growth.
Additionally,
an
improvement
in
surface
characteristics
observed,
demonstrated
shift
contact
angle
from
44
99°
reduction
water
vapor
transmission
rate
(WVTR)
4.82
2.31
g/h
m2.
For
agronomic
application,
films
were
experimentally
applied
maize
plants
pots.
results
positive,
showing
mulches
effectively
supported
growth
plants.
Further,
signs
initial
degradation
observed
after
5
days,
reached
level
50–55%
30
days
under
natural
conditions.
Thus,
work
has
provided
new
insights
expanding
application
range
biobased
materials.
ACS ES&T Engineering,
Journal Year:
2024,
Volume and Issue:
4(9), P. 2177 - 2185
Published: Aug. 5, 2024
This
study
tested
polyhydroxyalkanoate
(PHA)
production
by
usingPseudomonas
putida
KT2440
in
a
5
L
lab-scale
bioreactor,
employing
an
A
cell
dry
mass
concentration
of
8.69
±
0.45
g/L
was
achieved
with
volatile
fatty
acid
(VFA)
removal
efficiency
81.75
3.08%.
Remarkably,
this
the
highest
PHA
fraction
(34.81
1.67%).
Analysis
revealed
that
poly(3-hydroxybutyrate)
(PHB)
contributes
83.6
to
86.1%
total
followed
poly(3-hydroxyhexanoate)
(PHHx)
(6.6–8.6%)
and
poly(3-hydroxy-2-methylvalerate)
(PH2MV)
(4.9–8.3%).
Thermogravimetric
analysis
showed
polymer
started
degrading
at
temperature
150
°C
maximum
weight
loss
occurring
approximately
270
°C.
Differential
scanning
calorimetry
two
peaks
corresponding
melting
polymer:
140.6,
154.87
suggests
resulting
blend
different
copolymers.
outcome
is
poised
advance
development
unified
methods
within
circular
bioeconomy,
enhancing
both
economic
environmental
advantages.
