Mechanism of Eriochloa villosa (Thunb.) Kunth Resistance to Nicosulfuron DOI Creative Commons
Jing Guo, Zeqian Xu, Ting Jiao

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(10), P. 2210 - 2210

Published: Sept. 25, 2024

Eriochloa villosa (Thunb.) Kunth, the main weed in corn fields, has gradually developed resistance to nicosulfuron due continuous and extensive application. We identified a biotype showing ALS inhibitor with resistant index 13.83, but without any target spot mutation. Herein, transcriptome sequencing was used analyze differences gene expression at transcriptional level between nicosulfuron-resistant E. HEK-40 varieties sensitive HEK-15 varieties. The comparison revealed 9931 DEGs after application, of which 5426 4505 genes were up-regulated down-regulated, respectively. Some contigs related metabolic based on differential via RNA-Seq, includes ABC transporters (ko02010), glucosinolate biosynthesis (ko00966), 2-oxocarboxylic acid metabolism (ko01210), alanine, aspartate, glutamate pathways (ko00250). Seven CYP450 genes, four GST ten transporter two GT identified. 10 candidate screened validated using q-PCR. This validation indicates that activities associated P450 enzymes, transporters, glutathione S-transferases (GST) may play role conferring resistance, is important for reducing impact weeds fields ensuring food security.

Language: Английский

Biodegradation of atrazine and nicosulfuron by Streptomyces nigra LM01: Performance, degradative pathway, and possible genes involved DOI Creative Commons

Shengchen Zhao,

Jihong Wang

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 471, P. 134336 - 134336

Published: April 16, 2024

Microbial herbicide degradation is an efficient bioremediation method. In this study, a strain of Streptomyces nigra, LM01, which efficiently degrades atrazine and nicosulfuron, was isolated from corn field using direct isolation The effects the identified on two herbicides were investigated optimized artificial neural network. maximum rates S. nigra LM01 58.09% 42.97% for respectively. rate in soil reached 67.94% when concentration 108 CFU/g after 5 d less effective than that nicosulfuron. Whole genome sequencing helped elucidate possible pathways protein sequences aligned with degraded proteins by National Center Biotechnology Information platform. sequence (GE005358, GE001556, GE004212, GE005218, GE004846, GE002487) highest query cover retained docked small-molecule ligands herbicides. results revealed binding energy −6.23 kcal/mol between GE005358 ligand −6.66 GE002487 nicosulfuron ligand. Atrazine are commonly used remain environment several hundred days. They often combination fields can cause severe damage to non-corn crops, including soybeans wheat. This phenomenon interferes crop rotation not conducive sustainable agriculture. soil, it degrading study elucidated mechanism detail, providing reference understanding environment.

Language: Английский

Citations

13
Investigation of bioremediation mechanism of nicosulfuron-contaminated soil by highly efficient degrading bacterial consortium YM1: Analysis of degradation genes and microbial community structure DOI

Meiqi Dong,

Yufeng Xiao,

Siya Wang

et al.

Applied Soil Ecology, Journal Year: 2025, Volume and Issue: 209, P. 106060 - 106060

Published: March 28, 2025

Language: Английский

Citations

0
Deciphering the role of nitric oxide in mitigation of systemic fungicide induced growth inhibition and oxidative damage in wheat DOI
Nabil Touzout, Adil Mihoub, Iftikhar Ahmad

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 364, P. 143046 - 143046

Published: Aug. 8, 2024

Language: Английский

Citations

2
Bioremediation of soils contaminated with nicosulfuron by the bacterial complex ES58 DOI
Bingbing Yang, Yufeng Xiao,

Meiqi Dong

et al.

Process Biochemistry, Journal Year: 2024, Volume and Issue: 146, P. 176 - 187

Published: July 17, 2024

Language: Английский

Citations

1
Degradation Characteristics of Nicosulfuron in Water and Soil by MnO2 Nano-Immobilized Laccase DOI Creative Commons

Wanlei Yue,

Xin Wang,

Jiale Zhang

et al.

Toxics, Journal Year: 2024, Volume and Issue: 12(8), P. 619 - 619

Published: Aug. 21, 2024

As a typical sulfonylurea herbicide, nicosulfuron is mainly used to control grass weeds and some broadleaf in corn fields. However, as the amount of use continues increase, it accumulates environment eventually becomes harmful ecosystem. In present study, new metallic nanomaterial, δ-MnO

Language: Английский

Citations

1
Nitric oxide application alleviates fungicide and ampicillin co-exposure induced phytotoxicity by regulating antioxidant defense, detoxification system, and secondary metabolism in wheat seedlings DOI
Nabil Touzout, Adil Mihoub,

Mahdia Boukheddimi

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 372, P. 123337 - 123337

Published: Nov. 19, 2024

Language: Английский

Citations

1
Deciphering the Role of Nitric Oxide in Mitigation of Systemic Fungicide Induced Growth Inhibition and Oxidative Damage in Wheat DOI
Nabil Touzout, Adil Mihoub,

Iftkhar Ahmad

et al.

Published: Jan. 1, 2024

Consento (CON) is a common fungicide used to control fungal diseases in potato crops. However, few studies have assessed the impact of systemic fungicides on non-target Nitric oxide (NO), signaling molecule, can mitigate oxidative stress, however related protective effects wheat under pesticide exposure are not clear. Therefore, we conducted hydroponic experiment examine role NO (100 µM) for improving plant tolerance terms growth, photosynthetic pigments, antioxidant defense, and metabolism system exposed CON stress. The results showed that at highest (4X) concentration significantly decreased fresh weight (46.89 %), shoot length (40.26 root (56.11 %) total chlorophyll contents (67.44 dose response relationship. Moreover, increased hydrogen peroxide, malondialdehyde, catalase, ascorbate peroxidase, glutathione-S-transferase, peroxidase activities while reduced glutathione (GSH) content. This ultimately impaired redox homeostasis cells, leading damage cell membrane. application mitigated phytotoxicity as reflected by pigments leaves. Indeed, alleviatory effect was able increase seedlings fungicide, which resulted increments detoxification enzymes activity, with enhanced GSH level. Interestingly, alleviated through phenylpropanoid pathway enhancing activity secondary (phenylalanine ammonia-lyase, polyphenol oxidase, associated metabolites (phenolic acids flavonoids) study conferred potential attenuate crop defense system, metabolisms. rational use pesticides mitigation gateway enhance food security protect environment.

Language: Английский

Citations

0
Mechanism of Eriochloa villosa (Thunb.) Kunth Resistance to Nicosulfuron DOI Creative Commons
Jing Guo, Zeqian Xu, Ting Jiao

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(10), P. 2210 - 2210

Published: Sept. 25, 2024

Eriochloa villosa (Thunb.) Kunth, the main weed in corn fields, has gradually developed resistance to nicosulfuron due continuous and extensive application. We identified a biotype showing ALS inhibitor with resistant index 13.83, but without any target spot mutation. Herein, transcriptome sequencing was used analyze differences gene expression at transcriptional level between nicosulfuron-resistant E. HEK-40 varieties sensitive HEK-15 varieties. The comparison revealed 9931 DEGs after application, of which 5426 4505 genes were up-regulated down-regulated, respectively. Some contigs related metabolic based on differential via RNA-Seq, includes ABC transporters (ko02010), glucosinolate biosynthesis (ko00966), 2-oxocarboxylic acid metabolism (ko01210), alanine, aspartate, glutamate pathways (ko00250). Seven CYP450 genes, four GST ten transporter two GT identified. 10 candidate screened validated using q-PCR. This validation indicates that activities associated P450 enzymes, transporters, glutathione S-transferases (GST) may play role conferring resistance, is important for reducing impact weeds fields ensuring food security.

Language: Английский

Citations

0