Linking Antibiotic Residues and Antibiotic Resistance Genes to Water Quality Parameters in Urban Reservoirs: A Seasonal Perspective

Environments, Journal Year: 2025, Volume and Issue: 12(3), P. 96 - 96

Published: March 18, 2025

The interaction between antibiotics and antibiotic resistance genes (ARGs) in freshwater ecosystems has become a critical environmental concern. This study investigates seasonal variations of sulfonamide tetracycline their relationship with ARGs three urban reservoirs Nanjing, China: Pingshan Forest Park, Shanhu Lake Wetland Zhaoqiao Reservoir. Sampling was conducted May September 2023 to assess water quality, concentrations, ARG abundance. A total 30 samples were analyzed regard physicochemical parameters, heavy metals, antibiotics. quantitative PCR assay used measure the abundance relative 16S rRNA gene. Sulfonamide concentrations ranged from 120 ng/L, while tetracyclines 50–160 ng/L. Notably, sulfamethazine decreased significantly two (Shanhu Zhaoqiao, p < 0.05), other showed minimal variation, indicating persistent contamination agricultural runoff wastewater discharge. lower than September, being cumulatively genes. Strong correlations (r > 0.7) observed parameters like dissolved oxygen pH. High levels areas without nearby hospitals or pharmaceutical companies, implicating agriculture as major pollution source. By analyzing across eutrophic China, we highlight drivers proliferation propose targeted mitigation strategies.

Language: Английский

Antibiotic resistance genes in plague ecosystems: Threatening the emergence of resistant plague

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 287, P. 117340 - 117340

Published: Nov. 1, 2024

The study aimed to investigate the prevalence of antibiotic resistance genes (ARGs) within ecosystem natural plague foci, assessing their potential impact on efficacy treatments. Employing 16S rRNA gene sequencing and high-throughput quantitative PCR, microbial communities ARGs were detected, with subsequent analysis interactions among ARGs, mobile genetic elements (MGEs), environmental factors, species. Tetracycline found be dominant, multidrug tetracycline primarily associated marmots ecological soil, while pikas predominantly harbored β-lactam ARGs. High detection rates observed for rpsl sul1, which are relevant streptomycin sulfonamides, antibiotics commonly used in treatment. total dissolved solids (TDS) soil significantly promoted presence tetR-02, Ni was inhibit vanHB. tnpA-03 MGE identified as a significant contributor dissemination aadE gene. high particularly poses risk main treatments plague. suggests that microbiomes may greatest factor emergence drug-resistant Yersinia pestis, given low misuse animals foci. Monitoring strain preparing alternative or combination therapy strategies based ARG pollution levels plague-affected areas is deemed necessary.

Language: Английский