Tea seedlings growth promotion by widely distributed and stress-tolerant PGPR from the acidic soils of the Kangra valley DOI Creative Commons
R. P. Thakur, Praveen Rahi, Ashu Gulati

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 28, 2025

This is the first report of widespread and stress-tolerant PGPR from tea rhizosphere Kangra valley. A total 493 rhizobacteria were isolated major tea-growing regions Molecular fingerprinting 160 distinct morphotypes using ARDRA ERIC techniques revealed intergenic intragenic variability, resulting in identification 52 rRNA 56 types belonging to 21 distantly related genera, identified by 16S gene sequencing. Bacillus constituted more than half genotypes, followed Pseudomonas, Burkholderia, Lysinibacillus, Citrobacter, Enterobacter, Paenibacillus. altitudinis, B. cereus, megaterium, subtilis subsp. inaquosorum, methylotropicus, Pseudomonas frederiksbergensis, P. mohnii, moreiii found be most common across various locations. Quantitative assaying 42 selected strains significant variations PGP activities ranging 55–624 µg/ml for tri-calcium phosphate (TCP) solubilization, 4–3145 nM α-ketobutyrate h/mg/protein ACC-deaminase activity, 2–85 IAA-like auxins production, 2–83% siderophore production. Nine out also solubilized aluminium (Al-P) iron (Fe–P). These efficient are suitable application soils, which generally low available phosphorus, a growth-limiting factor cultivation. Five highly showed robust growth under different abiotic stresses controlled conditions. Inoculum 5 stress tolerant increment 1.8–9.4%, 12–16.2%,18.1–30.3% 21.4–39.2% plant height, leaf number, fresh dry weight seedlings nursery conditions with 50% reduced NPK concentrations after one year inoculations, respectively. genotypes multifarious natural ability occur widely can useful developing microbial inoculants improving productivity.

Language: Английский

Tea seedlings growth promotion by widely distributed and stress-tolerant PGPR from the acidic soils of the Kangra valley DOI Creative Commons
R. P. Thakur, Praveen Rahi, Ashu Gulati

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 28, 2025

This is the first report of widespread and stress-tolerant PGPR from tea rhizosphere Kangra valley. A total 493 rhizobacteria were isolated major tea-growing regions Molecular fingerprinting 160 distinct morphotypes using ARDRA ERIC techniques revealed intergenic intragenic variability, resulting in identification 52 rRNA 56 types belonging to 21 distantly related genera, identified by 16S gene sequencing. Bacillus constituted more than half genotypes, followed Pseudomonas, Burkholderia, Lysinibacillus, Citrobacter, Enterobacter, Paenibacillus. altitudinis, B. cereus, megaterium, subtilis subsp. inaquosorum, methylotropicus, Pseudomonas frederiksbergensis, P. mohnii, moreiii found be most common across various locations. Quantitative assaying 42 selected strains significant variations PGP activities ranging 55–624 µg/ml for tri-calcium phosphate (TCP) solubilization, 4–3145 nM α-ketobutyrate h/mg/protein ACC-deaminase activity, 2–85 IAA-like auxins production, 2–83% siderophore production. Nine out also solubilized aluminium (Al-P) iron (Fe–P). These efficient are suitable application soils, which generally low available phosphorus, a growth-limiting factor cultivation. Five highly showed robust growth under different abiotic stresses controlled conditions. Inoculum 5 stress tolerant increment 1.8–9.4%, 12–16.2%,18.1–30.3% 21.4–39.2% plant height, leaf number, fresh dry weight seedlings nursery conditions with 50% reduced NPK concentrations after one year inoculations, respectively. genotypes multifarious natural ability occur widely can useful developing microbial inoculants improving productivity.

Language: Английский

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