Analysis of Nicotine Toxicity and Mechanisms of Senescence in Nucleus Pulposus Cells Using Network Toxicology and Molecular Docking Technique DOI Creative Commons

Chen Jiang,

Chao Song, Chaoqi Chen

et al.

JOR Spine, Journal Year: 2025, Volume and Issue: 8(2)

Published: March 31, 2025

ABSTRACT Aim Through the use of network toxicology, research sought to determine whether cellular senescence and associated molecular mechanisms in nicotine‐induced intervertebral disc degeneration (IVDD) were potentially harmful. Methods The primary chemical structure 105 targets action nicotine determined by using Swiss Target Prediction, Cell Age, PubChem databases. 855 IVDD genes found GEO Age datasets. Results After additional screening Cytoscape development, 9 key identified. Additionally, these targets' co‐expression pattern analysis protein interactions confirmed be identical. core primarily enriched positive regulation cell proliferation, telomere shortening, histone acetylation, senescence‐related processes, according gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG). KEGG signaling pathway also made it clear that Apelin route, nicotinate nicotinamide metabolism, cycle, apoptosis are all strongly linked senescence. We chose four with pathway—HDAC1, HDAC4, NAMPT, MYLK—for docking toxic substance nicotine. findings validated nicotine's strong affinity for targets. Conclusion All things considered, current indicates may contribute via controlling deacetylation process, pathway, pathways metabolism nicotinamide. theoretical foundation investigating is established.

Language: Английский

Analysis of Nicotine Toxicity and Mechanisms of Senescence in Nucleus Pulposus Cells Using Network Toxicology and Molecular Docking Technique DOI Creative Commons

Chen Jiang,

Chao Song, Chaoqi Chen

et al.

JOR Spine, Journal Year: 2025, Volume and Issue: 8(2)

Published: March 31, 2025

ABSTRACT Aim Through the use of network toxicology, research sought to determine whether cellular senescence and associated molecular mechanisms in nicotine‐induced intervertebral disc degeneration (IVDD) were potentially harmful. Methods The primary chemical structure 105 targets action nicotine determined by using Swiss Target Prediction, Cell Age, PubChem databases. 855 IVDD genes found GEO Age datasets. Results After additional screening Cytoscape development, 9 key identified. Additionally, these targets' co‐expression pattern analysis protein interactions confirmed be identical. core primarily enriched positive regulation cell proliferation, telomere shortening, histone acetylation, senescence‐related processes, according gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG). KEGG signaling pathway also made it clear that Apelin route, nicotinate nicotinamide metabolism, cycle, apoptosis are all strongly linked senescence. We chose four with pathway—HDAC1, HDAC4, NAMPT, MYLK—for docking toxic substance nicotine. findings validated nicotine's strong affinity for targets. Conclusion All things considered, current indicates may contribute via controlling deacetylation process, pathway, pathways metabolism nicotinamide. theoretical foundation investigating is established.

Language: Английский

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