Analysis of network pharmacology and experimental verification to explore the mechanism of Modified Guilu Erxian Glue in the treatment of low immune function DOI Creative Commons
Yue Wang, Haohao Wang,

Chunyu Gao

et al.

Pharmacological Research - Modern Chinese Medicine, Journal Year: 2022, Volume and Issue: 7, P. 100213 - 100213

Published: Dec. 27, 2022

Guilu Erxian Gum (龟鹿二仙胶, GEG) is a traditional Chinese medicine prescription, which widely used to treat low immunity and immune function impairment caused by chemotherapy. However, its potential pharmacological mechanism remains largely unknown. : The aim of this study was investigate the therapeutic effect modified (MGEG) on cyclophosphamide (Cy) induced immunosuppression in mice. Based network pharmacology, comprehensive hypofunction preliminarily discussed. Cyimmunosuppression model established observe MGEG compound injury best screened for analysis, "drug component" "disease drug target" networks were methods. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis conducted according common target between herbal determine biological process signal pathway. core components targets obtained pharmacology verified Western blot (WB). In vivo experiments showed that weight, thymus index, organ carbon particle clearance phagocytosis foot thickening level, macrophage natural killer cell killing activity immunosuppressed mice Cy significantly increased Codonopsis pilosula, Jiaowei Wuxing Powder (CGW) (p<0.05 or p<0.01). addition, CGW humoral cellular it predicted contains 125 bioactive components, involving 489 action targets, may be related immunosuppressive diseases. establishment protein interaction network, five central nodes identified as active factors involved major functions. David, ClueGO Metascape all proved plays an important role regulation function. By KEGG TNF signaling pathway considered one main pathways treatment immunosuppression, including wide range intracellular pathways. NF-κB regulates inflammation WB promoted expression further confirms can enhance body's path activation. experimental verification, improve revealed, providing theoretical basis development medicine.

Language: Английский

Traditional Tibetan Medicine Twenty-Five Wei’er Tea Pills Ameliorate Rheumatoid Arthritis Based on Chemical Crosstalk Between Gut Microbiota and the Host DOI Creative Commons
Zixuan Li,

Lijuan Nie,

Yong Li

et al.

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: Feb. 10, 2022

Twenty-Five Wei’er Tea Pills (TFP), a traditional Tibetan medicine, has shown to have promising therapeutic effect in patients with Rheumatoid arthritis (RA), as well being safe. Nonetheless, there been limited pharmacological studies that explored this option. As gut microbiota proven critical role the pathogenesis of RA, study aims explore and reveal relevant ways by which TFP interacts chemical crosstalk between microbiome its host. 16S rRNA sequencing, combined un-targeted metabolomics, were conducted on collagen-induced (CIA) rats. CIA model rats treated showed significant improvement weight gain, pathological phenomena joints, decreased serum levels TNF-α, IL-6 increased level IL-4 IL-10. Significant dysfunction alteration metabolites observed rats, restored treatment. Coherence analysis indicated modulated pathways histidine metabolism, phenylalanine alanine, aspartate, glutamate amino sugar nucleotide metabolism owing abundances Lactobacillus , Bacteroides Prevotellaceae _UCG-001 Christensenellaceae _R-7_group microflora. The corresponding involved L-histidine, histamine, phenylethylamine, asparagine, L-aspartic acid, D-fructose 1-phosphate, D-Mannose 6-phosphate, D-Glucose Glucose 1-phosphate. In conclusion, reveals ameliorative effects RA through exists host, also further enriches our understandings RA.

Language: Английский

Citations

23
Active metabolites combination therapies: towards the next paradigm for more efficient and more scientific Chinese medicine DOI Creative Commons
Quan Gao, Hao Wu, Min Chen

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: April 18, 2024

Traditional Chinese medicine (TCM) formulae have been studied extensively in various human diseases and proven to be effective due their multi-component, multi-target advantage. However, its active metabolites are not clear the specific mechanisms well established, which limits scientific application. Recently, combination therapies attracting increasing attention from community past few years considered as next paradigm drug discovery. Here, we tried define a new concept of "active (AMCT)" rules elucidate how bioactive TCMs produce synergistic effects this review. The AMCT integrate multidisciplinary technologies like molecular biology, biochemistry, pharmacology, analytical chemistry pharmacodynamics, etc. Meanwhile, emerging such multi-omics combined analysis, network artificial intelligence conduce better these disease treatment, provides insights for development novel drugs. will hopefully further guide drugs that promote modernization international needs TCM.

Language: Английский

Citations

4
Network pharmacology combined with affinity ultrafiltration to elucidate the potential compounds of Shaoyao Gancao Fuzi Decoction for the treatment of rheumatoid arthritis DOI

Weiliang Fu,

Chengyu Shentu,

Dan Chen

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 330, P. 118268 - 118268

Published: April 26, 2024

Language: Английский

Citations

4
Investigation into the anti-inflammatory mechanism of Pothos chinensis (Raf.) Merr. By regulating TLR4/MyD88/NF-κB pathway: Integrated network pharmacology, serum pharmacochemistry, and metabolomics DOI Creative Commons
Guanlin Xiao, Minjuan Yang, Zhihao Zeng

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 334, P. 118520 - 118520

Published: July 2, 2024

Inflammation is directly related to disease progression and contributes significantly the global burden of disease. Pothos chinensis (Raf.) Merr. (PCM) commonly used in Yao medicine China treat tumors, orthopedic illnesses such as knee osteoarthritis, rheumatic bone discomfort. PCM was found have significant anti-inflammatory properties previous studies.

Language: Английский

Citations

4
Quercetin, a Compound of the Total Flavonoids of Periploca forrestii Schltr., Ameliorates Rheumatoid Arthritis by Targeting TNF-α DOI Creative Commons
Shuaishuai Chen,

Wei-Na Xue,

Zhongxiu Wu

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 2879 - 2898

Published: Feb. 1, 2025

The total flavonoids of Periploca forrestii Schltr. (TFPF) exhibit notable anti-rheumatoid arthritis (RA) properties, highlighted by their inhibitory action on TNF-α. This study investigates the specific compounds TFPF responsible for TNF-α inhibition and delineates mechanism action. L929 cells luciferase-based assays were used to assess anti-TNF-α activity. Additionally, MH7A Wistar rats employed establish in vitro vivo models RA. Chemical chromatography, thermal proteome profiling (TPP), molecular docking, surface plasmon resonance (SPR), cellular shift (CETSA), drug affinity responsive target stability tests (DARTS), transcriptomic analysis potent molecules protein targets pathways. H&E staining, immunofluorescence ELISA, Western blot pharmacodynamic studies these molecules. mitigated injury inhibited luciferase expression, both which induced Among eight identified, quercetin exhibited most Quercetin improved reduced cell apoptosis rate treated with TPP revealed interaction between TNF-α, was verified SPR, CETSA, DARTS assays. Transcriptomic TNF signaling pathway, suggesting that may inhibit activation NF-κB pathway. suppressed phosphorylation IκBα p65, blocked p65 nuclear translocation, mRNA expression COX-2, INOS, IL-6, IL-1β. Moreover, decreased inflammation joint damage Quercetin, a compound TFPF, interacts inhibits activity thereby suppressing TNF-α/NF-κB pathway alleviating symptoms These findings position as promising inhibitor treatment

Language: Английский

Citations

0
Exploration of scientific connotation of “Yin-Jing” medical properties of Cyathula Officinalis via potentiating therapeutic effect, guidance and targetability DOI
Haifeng Jiang,

Jun-Hong Chai,

Lan Zhou

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119629 - 119629

Published: March 1, 2025

Language: Английский

Citations

0
Regulating Rheumatoid Arthritis From the Perspective of Metabolomics: A Comprehensive Review DOI
Yujiao Liang,

Yingxue Cheng,

Jinjun Ji

et al.

International Journal of Rheumatic Diseases, Journal Year: 2025, Volume and Issue: 28(3)

Published: March 1, 2025

ABSTRACT Rheumatoid arthritis (RA) is a severe inflammatory autoimmune disease with metabolic changes. RA patients have abnormalities in glycolysis, amino acid metabolism, choline and fatty synthesis. The differential metabolites individuals of animal models were explored to find the potential biomarkers for risk prediction, diagnosis, prognosis perspective metabolism. Moreover, we discussed changes related after treatment anti‐rheumatic drugs, Traditional Chinese Medicine (TCM) explore promising metabolites. In addition, immunological mechanism TCM from metabolism was also clarified. For perspectives research application beneficial clinic, relevant technologies focuses future studies field been proposed accordingly.

Language: Английский

Citations

0
Therapeutic effects and mechanism of action of lavender essential oil on atopic dermatitis by modulating the STAT3/RORγt pathway DOI Creative Commons

Jiawei Duan,

Jinkai Li, Yujiao Wang

et al.

Arabian Journal of Chemistry, Journal Year: 2023, Volume and Issue: 17(2), P. 105525 - 105525

Published: Dec. 6, 2023

Atopic dermatitis (AD) is a common skin disorder characterized by inflammation caused an imbalance in the immune response. The efficacy of lavender essential oil treating atopic has been demonstrated; however, its specific mechanism action and active components remain unknown. Therefore, this study investigated therapeutic effects possible LEO on AD 2,4-dinitrochlorobenzene (DNCB)-induced mice model. To determine LEO, serum levels cytokines IL-6 TNF-α were tested Kunming mice, network pharmacology was used to predict targets mechanisms treatment, after which combined with metabolomics construct complex response–enzyme–gene networks investigate their potential associations. Based predicted mechanisms, tissues further examined immunohistochemistry immunoblotting analysis, epidermis stained hematoxylin-eosin (HE) toluidine blue (TB). significantly suppressed basal DNCB-induced model that Th17 cell differentiation critical pathway for LEO-based treatment. As associated pathway, experiments verified reduced protein expression IL-17A、 p-JAK、p-STAT3, RORγt but increased Foxp3. Additionally, results analysis showed could improve two metabolic pathways, namely, linoleic acid metabolism arachidonic metabolism, regulating pathway; identify key metabolites (linoleic acid, acid); regulate differential genes (PTGS1, HPGD). may alleviate inhibiting STAT3/RORγt differentiation, reducing inflammatory chemokines improving vivo.

Language: Английский

Citations

8
Panlongqi tablet suppresses adjuvant-induced rheumatoid arthritis by inhibiting the inflammatory reponse in vivo and in vitro DOI
Xiaofeng Niu,

Yajie Yang,

Jinjin Yu

et al.

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 308, P. 116250 - 116250

Published: Feb. 13, 2023

Language: Английский

Citations

7
Integrated network pharmacology and metabolomics reveal the mechanisms of Jasminum elongatum in anti-ulcerative colitis DOI Creative Commons

Jinyan Qiu,

Guanlin Xiao, Minjuan Yang

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 17, 2023

Abstract Jasminum elongatum (JE), an ethnic Chinese medicine, is widely used in the Lingnan region of China, because its analgesic and antidiarrheal action, as well anti-inflammatory effects gastrointestinal diseases. However, whether JE could against ulcerative colitis (UC) remains unclear. This research aims to reveal treating UC clarify underlying mechanism. We 2.5% dextran sulfate sodium (DSS)-induced mice (C57BL/6J) evaluate therapeutic JE. Metabolomics serum network pharmacology were combined draw target-metabolite pathways. Apart from that, targets associated pathways confirmed, mechanism action was made clear, using immunohistochemistry. The pharmacodynamic results, including disease activity index (DAI), histological evaluation, inflammatory cytokines colon tissues, demonstrated that significantly relieved physiological pathological symptoms UC. Network analysis indicated 25 core targets, such TNF, IL-6, PTGS2 RELA, four key pathways, NF-κB signaling pathway arachidonic acid metabolism pathway, which connections between identified 45 endogenous differential metabolites 9 metabolic by enrichment, with being main consistent prediction pharmacology. IκB, p65 COX-2 this study for first time reverses DSS-induced via IκB/p65/COX-2/arachidonic pathway. reveals complex mechanisms on provides a new approach identifying pharmacological natural medicines

Language: Английский

Citations

7