Optimized Centrifugal Partition Chromatography (CPC) Protocol for Isolation of Urease Inhibitors: Magnoflorine and Berberine from Berberis vulgaris Extracts

Separations, Journal Year: 2024, Volume and Issue: 11(4), P. 94 - 94

Published: March 25, 2024

In recent years, an increasing interest in phytotherapy has been observed. Parallel to the research on total extracts of plant material, numerous studies activity single molecules derived from plants are being conducted address their mechanisms action and determine active doses eventual interactions. Despite this phenomenon, isolation individual compounds is a bottleneck due its difficulty cost. This work presents results careful optimization magnoflorine berberine (isoquinoline alkaloids) recovery commonly distributed shrub, Berberis vulgaris, growing Poland Georgia, using CPC. Both known for medicinal properties, which makes methodology important area research. Additionally, CPC ability isolate high-quality large quantities, it effective easy-to-commercialize method. For successful separation, biphasic solvent system composed hexane, butanol, ethanol, water ratio (3:12:4:16 v/v/v/v) was used ascending mode, together with flow rate 8 mL/min rotation speed 1600 rpm. The method selective both compounds, delivered good root stem plant. qualitative composition alkaloids studied determined by HPLC-ESI-QTOF-MS/MS confirmed presence berberine, magnoflorine, jatrorhizine, palmatine group isoquinolines. isolates, were subjected Helicobacter pylori growth inhibition assay urease test assess whether, next previously proved anticancer these characterized H. inhibition. MGN found exhibit inhibitory potential against (IC50 = 25 mg/L).

Language: Английский

Natural Products Based on Correa's Cascade for the Treatment of Gastric Cancer Trilogy: Current Status and Future Perspective

Journal of Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 15(2), P. 101075 - 101075

Published: Aug. 19, 2024

Gastric carcinoma (GC) is a malignancy with multifactorial involvement, multicellular regulation, and multistage evolution. The classic Correa's cascade of intestinal GC specifies trilogy malignant transformation the gastric mucosa, in which normal mucosa gradually progresses from inactive or chronic active gastritis (Phase I) to precancerous lesions II) finally III). highlights evolutionary pattern importance early intervention prevent mucosa. Intervening mucosal lesions, i.e., Phase I II, will be key strategy treat GC. Natural products (NPs) have been an important source for drug development due abundant sources, tremendous safety, multiple pharmacodynamic mechanisms. This review first investigate summarize multi-step effects regulatory mechanisms NPs on carcinogenesis. In phase I, modulate Helicobacter pylori urease activity, motility, adhesion, virulence factors, resistance, thereby inhibiting H. pylori-induced inflammation oxidative stress, facilitating ulcer healing. pathways mediators regulating cell cycle, apoptosis, autophagy, angiogenesis reverse lesions. III, suppress proliferation, migration, invasion, angiogenesis, cancer stem cells, induce apoptosis enhance chemotherapeutic sensitivity treatment contrast existing work, we hope uncover sequential therapeutic phases development, providing new ideas prevention, treatment, development.

Language: Английский

Corydalis yanhusuo extract and its pharmacological substances alleviate food allergy by inhibiting mast cells activation via PLC/PKC/STAT3 pathway

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 337, P. 118809 - 118809

Published: Sept. 7, 2024

Language: Английский