Investigation of the effects of Periplaneta americana (L.) extract on ischemic stroke based on combined multi-omics of gut microbiota DOI Creative Commons
Xin Yang, Hong Chen,

Tianmin Guan

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 28, 2024

Ischemic stroke (IS) is a highly lethal type of cardiovascular and cerebrovascular disease. Improving survival rates promoting recovery in patients with IS pose significant challenges, however, recent research has identified the gut–brain axis as therapeutic target. In this study, we evaluated regulatory effect Periplaneta americana (L.) extract (PAS840), which established anti-inflammatory, antioxidant, neuroprotective effects, on gut microbiota using rat model temporary middle cerebral artery occlusion (tMCAO). We protective effects PAS840 brain damage rats through TTC (triphenyltetrazolium chloride), Nissl staining, pathological section analysis. Additionally, investigated impact metabolites 16S rRNA sequencing, untargeted metabolomics contents, transcriptomics analyses tissues to explore its mechanism action. intervention resulted changes microbiota, including an increase abundance probiotic flora, decrease harmful metabolite profiles. It also attenuated damage, decreased platelet activity, inhibited oxidative stress genes related inflammation, improved neurological function rats. These findings suggest that preventive against via by regulating metabolites. Accordingly, candidate drug for further research.

Language: Английский

Neural Stem Cell-Derived Small Extracellular Vesicles: key Players in Ischemic Stroke Therapy – A Comprehensive Literature Review DOI Creative Commons
Zhihan Zhu, Qiankun Zhang, Jia Feng

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 4279 - 4295

Published: May 1, 2024

Ischemic stroke, being a prominent contributor to global disability and mortality, lacks an efficacious therapeutic approach in current clinical settings. Neural stem cells (NSCs) are type of cell that only found inside the nervous system. These can differentiate into various kinds cells, potentially regenerating or restoring neural networks within areas brain have been destroyed. This review begins by providing introduction existing approaches for ischemic followed examination promise limits associated with utilization NSCs treatment stroke. Subsequently, comprehensive overview was conducted synthesize literature on underlying processes cell-derived small extracellular vesicles (NSC-sEVs) transplantation therapy context mechanisms encompass neuroprotection, inflammatory response suppression, endogenous nerve vascular regeneration facilitation. Nevertheless, translation NSC-sEVs is hindered challenges such as inadequate targeting efficacy insufficient content loading. In light these limitations, we compiled advancements utilizing modified treating stroke based methods vesicle modification. conclusion, examining NSC-sEVs-based anticipated be both fundamental applied investigations about

Language: Английский

Citations

4
UCMSCs-derived exosomal SLIT2 alleviates ischemic stroke through the β-catenin/TCF4/USP20 signaling pathway DOI

Rongjun Xiao,

Qingsong Wang,

Jun Peng

et al.

International Journal of Neuroscience, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: April 24, 2025

Ischemic stroke (IS) is a disease that causes necrosis of brain tissues by inadequate blood supply to the brain. Umbilical cord mesenchymal stem cells (UCMSCs)-derived exosomes (UCMSCs-Exo) have been reported alleviate IS, and slit guidance ligand 2 (SLIT2) could promote neurological repair after IS. The aim this research was explore potential mechanism UCMSCs-derived exosomal SLIT2 on IS progression. middle cerebral artery occlusion (MCAO) rat oxygen glucose deprivation/reperfusion (OGD/R)-induced cellular models were established, then treated with UCMSCs-Exo. Cell viability apoptosis explored cell counting kit-8 (CCK-8) assay flow cytometry, respectively. expressions ubiquitin specific peptidase 20 (USP20) related apoptotic proteins determined using Western blot. Immunofluorescence immunohistochemistry performed evaluate effect β-catenin nuclear translocation. association between transcription factor 4 (TCF4) USP20 investigated chromatin immunoprecipitation (ChIP) dual-luciferase reporter assys. In OGD/R-induced model, increased viability, decreased promoted Besides, agonist (SKL2001) facilitated promoting TCF4 binding promoter. Finally, upregulated inhibited damage. MCAO mitigated translocation, which activated TCF4/USP20 pathway inhibit apoptosis. transcriptionally expression, thereby attenuating neuroncell damage ultimately leading inhibition

Language: Английский

Citations

0
Electroacupuncture negatively regulates the Nesfatin-1/ERK/CREB pathway to alleviate HPA axis hyperactivity and anxiety-like behaviors caused by surgical trauma DOI Creative Commons
Jiayuan Zheng, Yu Wang,

Chi Zhang

et al.

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Aug. 17, 2024

Abstract Background Hyperactivity of the hypothalamic–pituitary–adrenal (HPA) axis constitutes a pivotal response by surgical trauma, manifesting as critical aspect acute stress reaction. This hyperactivity resulted in adverse outcomes and is often associated with increased postoperative anxiety. Increased evidence suggests that Nesfatin-1 plays crucial role responses stress-related psychiatric disorders. Electroacupuncture (EA) widely used to alleviate anxiety, although its mechanism action remains unclear. study aimed assess mechanisms which hypothalamic contribute alleviation HPA anxiety EA. Methods Partial hepatectomy (HT) was performed simulate EA applied at Zusanli (ST36) Sanyinjiao (SP6). The levels Nesfatin-1, c-Fos, corticotropin-releasing hormone (CRH) were detected, serum adrenocorticotropic (ACTH) corticosterone (CORT) regarded indicators activity. Anxiety assessed through open field tests (OFT), elevated plus maze (EPM), light–dark box (LDBT). To investigate expression modulated using stereotactic viral injections or plasmid transfections. Transcriptome sequencing employed explore downstream signaling pathways Nesfatin-1. Additionally, brain cannula implantation facilitate targeted drug administration. Results Our findings demonstrated reduced overexpression CRH well ACTH CORT. it alleviated anxiety-like behaviors resulting from trauma. We observed paraventricular nucleus (PVN) triggered Conversely, knocking down PVN reversed these effects caused identified extracellular regulated protein kinases (ERK)/cAMP-response element binding (CREB) pathway key mediator impacts trauma on hypothalamus. Both vivo vitro studies showed activated ERK/CREB pathway. Furthermore, administering ERK CREB inhibitors into mitigated induced Finally, decrease phosphorylation PVN. Conclusion alleviates inhibition Nesfatin-1/ERK/CREB

Language: Английский

Citations

2
Metabolomics-Based Study of the Protective Effect of 4-Hydroxybenzyl Alcohol on Ischemic Astrocytes DOI Open Access
Tian Xiao,

Xingzhi Yu,

Jie Tao

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9907 - 9907

Published: Sept. 13, 2024

Ischemic stroke is a common and dangerous disease in clinical practice. Astrocytes (ASs) are essential for maintaining the metabolic balance of affected regions during process. 4-Hydroxybenzyl alcohol (4HBA) from

Language: Английский

Citations

1
Periplaneta americana L. extract exerts neuroprotective effects by inhibiting endoplasmic reticulum stress via AKT-dependent pathway in experimental models of Parkinson’s disease DOI Creative Commons

Ting Cao,

Xuelian Wang,

Jiangyan Rao

et al.

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 13, 2024

Abstract Background Parkinson’s disease (PD) is a chronic neurodegenerative disorder that currently has no curable strategies. More and more evidence suggests endoplasmic reticulum (ER) stress plays an essential role in PD pathogenesis. Periplaneta americana L. ( P. ) traditional Chinese medicine with diverse therapeutic properties. This study aims to investigate the neuroprotective effect underlying mechanism of vitro vivo models. Methods The exposure SH-SY5Y cells 1-methyl-4-phenyl-pyridinium (MPP + was used as model. MTT assay, Hoechst staining, Calcein AM-PI staining flow cytometry were performed measure cell viability apoptosis. DCFH-DA JC-1 assay intracellular ROS mitochondrial membrane potential (Δψm), respectively. Western-blot immunostaining conducted detect expression key molecules related ER stress. For model induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydro-pyridine (MPTP), motor function mice assessed behavioral tests, level TH examined western-blot immunostaining, measured western-blot. Results ethanol extract (PAE) concentration-dependently inhibited MPP -induced loss increased viability. PAE also remarkably attenuated accumulation, decline Δψm well excessive effects could be blocked inducer trimethylamine N-Oxide or activator tunicaymycin, while antioxidant N-Acetyl-L-cysteine inhibitor sodium 4-phenylbutyrate mimicked PAE. Furthermore, we found activate AKT/GSK3β/β-catenin pathway. on production, AKT MK-2206. In model, significantly improved function, prevented dopaminergic neuronal substantia nigra striatum MPTP-treated mice. Similarly, abolished Conclusions Our results suggest exerts through inhibiting via AKT-dependent may represent promising agent for treatment worthy further being exploited.

Language: Английский

Citations

1
The Essential Role of Angiogenesis in Adenosine 2A Receptor Deficiency-mediated Impairment of Wound Healing Involving c-Ski via the ERK/CREB Pathways DOI Creative Commons
Yan Peng,

Renping Xiong,

Bo Wang

et al.

International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(11), P. 4532 - 4550

Published: Jan. 1, 2024

Adenosine receptor-mediated signaling, especially adenosine A

Language: Английский

Citations

0
Differential Effects of Extracellular Vesicles from Two Different Glioblastomas on Normal Human Brain Cells DOI Creative Commons
Mary Wang,

Arin N. Graner,

Bryne Knowles

et al.

Neurology International, Journal Year: 2024, Volume and Issue: 16(6), P. 1355 - 1384

Published: Nov. 6, 2024

Glioblastomas (GBMs) are dreadful brain tumors with abysmal survival outcomes. GBM extracellular vesicles (EVs) dramatically affect normal cells (largely astrocytes) constituting the tumor microenvironment (TME). We asked if EVs from different patient-derived spheroid lines would differentially alter recipient cell phenotypes. This turned out to be case, net outcome of treatment nonetheless converging on increased tumorigenicity.

Language: Английский

Citations

0
Investigation of the effects of Periplaneta americana (L.) extract on ischemic stroke based on combined multi-omics of gut microbiota DOI Creative Commons
Xin Yang, Hong Chen,

Tianmin Guan

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 28, 2024

Ischemic stroke (IS) is a highly lethal type of cardiovascular and cerebrovascular disease. Improving survival rates promoting recovery in patients with IS pose significant challenges, however, recent research has identified the gut–brain axis as therapeutic target. In this study, we evaluated regulatory effect Periplaneta americana (L.) extract (PAS840), which established anti-inflammatory, antioxidant, neuroprotective effects, on gut microbiota using rat model temporary middle cerebral artery occlusion (tMCAO). We protective effects PAS840 brain damage rats through TTC (triphenyltetrazolium chloride), Nissl staining, pathological section analysis. Additionally, investigated impact metabolites 16S rRNA sequencing, untargeted metabolomics contents, transcriptomics analyses tissues to explore its mechanism action. intervention resulted changes microbiota, including an increase abundance probiotic flora, decrease harmful metabolite profiles. It also attenuated damage, decreased platelet activity, inhibited oxidative stress genes related inflammation, improved neurological function rats. These findings suggest that preventive against via by regulating metabolites. Accordingly, candidate drug for further research.

Language: Английский

Citations

0