Cell Biology and Toxicology,
Journal Year:
2024,
Volume and Issue:
41(1)
Published: Dec. 20, 2024
The
occurrence
of
severe
myocardial
ischemia/reperfusion
(I/R)
injury
is
associated
with
the
clinical
application
reestablishment
technique
for
heart
disease,
and
understanding
its
underlying
mechanisms
currently
an
urgent
issue.
Prior
investigations
have
demonstrated
potential
enhancement
MIRI
through
EGR1
suppression,
although
precise
regulatory
pathways
require
further
elucidation.
core
focus
this
investigation
to
examine
molecular
regulates
mitophagy-mediated
cell
pyroptosis
impact
on
MIRI.
Cardiomyocyte
hypoxia/reoxygenation
(H/R)
models
mouse
I/R
were
used
investigate
involvement
in
regulating
injury.
research
outcomes
that
under
H/R
conditions,
expression
was
upregulated
inhibited
JAK2/STAT3
pathway,
leading
enhanced
mitophagy
disrupted
mitochondrial
fusion/fission
dynamics,
ultimately
resulting
pyroptosis.
Further
revealed
upregulation
mediated
by
methyltransferase
like
3
(METTL3)-mediated
m6A
modification
mRNA
depended
binding
insulin
growth
factor
2
mrna
protein
(IGF2BP2)
N6-methyladenosine
(m6A)
site
enhance
stability.
In
vivo
animal
experiments
confirmed
METTL3
IGF2BP2
suppressed
activation
janus
kinase
(JAK2)
/signal
transducer
activator
transcription
(STAT3)
thereby
inhibiting
mitophagy,
disrupting
promoting
pyroptosis,
exacerbating
Pharmacological Research,
Journal Year:
2024,
Volume and Issue:
209, P. 107466 - 107466
Published: Oct. 15, 2024
Myocardial
ischemia
causes
extensive
damage,
further
exacerbated
by
reperfusion,
a
phenomenon
called
myocardial
ischemia/reperfusion
injury
(MIRI).
Nowadays,
the
pathological
mechanisms
of
MIRI
have
received
attention.
Oxidative
stress,
multiple
programmed
cell
deaths,
inflammation
and
others
are
all
essential
contributing
to
MIRI.
Mitochondria
energy
supply
centers
cells.
Numerous
studies
found
that
abnormal
mitochondrial
function
is
an
"culprit"
MIRI,
mitophagy
mediated
phosphatase
tensin
homolog
(PTEN)-induced
kinase
1
(PINK1)/Parkin
signaling
pathway
integral
part
maintaining
function.
Therefore,
exploring
association
between
PINK1/Parkin
pathway-mediated
crucial.
This
review
will
mainly
summarize
crucial
role
in
MIR-induced
several
various
potential
interventions
affect
mitophagy,
thus
ameliorating
Cancer Management and Research,
Journal Year:
2025,
Volume and Issue:
Volume 17, P. 23 - 34
Published: Jan. 1, 2025
Background:
Cancer
immunotherapy
is
an
advanced
therapeutic
approach
that
harnesses
the
body's
immune
system
to
target
and
eliminate
tumor
cells.
Traditional
Chinese
medicine
(TCM),
with
a
history
rooted
in
centuries
of
clinical
practice,
plays
crucial
role
enhancing
responses,
alleviating
cancer-related
symptoms,
reducing
risks
infections
complications
cancer
patients.
Methodology:
This
review
systematically
examines
current
literature
on
TCM-based
formulations
immunotherapy.
It
explores
mechanisms
by
which
TCM
augments
particularly
focusing
how
these
influence
both
innate
adaptive
immunity.
Various
herbs
compounds,
their
active
ingredients,
application
prevention
treatment
were
analyzed
through
integrated
preclinical
studies,
trials,
molecular
mechanistic
investigations.
Results:
contribute
therapy
modulating
internal
environment
improve
defense.
They
enhance
system's
ability
detect
destroy
cells,
promote
apoptosis
inhibit
growth
metastasis,
augment
effectiveness
conventional
treatments.
The
highlights
specific
have
demonstrated
significant
anti-cancer
properties,
including
strengthen
responses
provide
synergistic
effects
existing
therapies.
Conclusion:
TCM-derived
represent
promising
addition
anti-tumor
immunity
are
multifaceted,
involving
modulation
cell
activity,
induction,
suppression
progression.
As
evolves,
integration
into
regimens
may
offer
enhanced
efficacy,
reduced
side
effects,
improved
overall
outcomes
for
Further
research
needed
fully
elucidate
potential
safety
immunotherapeutic
strategies
care.
Keywords:
traditional
medicine,
TCM,
cancer,
nanocarriers,
ability,
therapy,
microenvironment,
drug
delivery
systems
Journal of Cardiothoracic Surgery,
Journal Year:
2025,
Volume and Issue:
20(1)
Published: Feb. 19, 2025
The
hypoxia/reoxygenation
(H/R)-induced
pyroptosis
of
cardiomyocytes
plays
a
crucial
role
in
the
pathogenesis
myocardial
infarction
(MI).
miR-155-5p
represents
promising
target
for
MI
therapy.
However,
its
involvement
H/R-induced
remains
unclear.
H/R
exposed
rat
cardiomyocyte
H9c2
was
utilized
as
vitro
model,
and
expression
levels
SIRT1
cells
were
modulated
through
cell
transfection
experiments.
Cell
proliferative
activity
assessed
using
counting
kit-8
assay.
Supernatant
lactate
dehydrogenase
(LDH)
determined
colorimetry.
living
dead
observed
via
Calcin-AM/PI
staining.
Levels
supernatant
interleukin
(IL)-1β
IL-18
measured
ELISA
silent
information
regulator
1
(SIRT1)
mRNA
detected
by
qRT-PCR.
protein
SIRT1,
NLRP3,
N-terminal
gasdermin
D
(GSDMD-N),
Cleaved
caspase-1
evaluated
Western
blot
analysis.
targeted
regulatory
relationship
between
verified
dual
luciferase
reporter
gene
proliferation
induced
attenuated,
accompanied
severe
injury,
increased
death,
release
substantial
amount
pro-inflammatory
cytokines
IL-1β
IL-18.
In
addition,
stimulation
resulted
upregulation
downregulation
cells.
Suppression
or
overexpression
exhibited
ameliorative
effects
on
cellular
injury
inhibited
NLRP3
inflammasome-mediated
pyroptosis.
dual-luciferase
assay
confirmed
direct
targeting
Furthermore,
partial
reversal
inhibitory
effect
inhibitor
upon
interference
with
expression.
Inhibition
alleviates
SIRT1-mediated
activation
inflammasome.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(6), P. 2589 - 2589
Published: March 13, 2025
The
heavy
metal
cadmium
(Cd)
affects
the
global
livestock
production
economy
mainly
through
contamination
of
feed
raw
materials
and
secondary
in
processing,
it
also
poses
a
serious
threat
to
food
safety
human
health.
nucleotide-binding
oligomerization
domain-like
pyrin-domain-containing
protein
3
(NLRP3)
inflammasome
is
key
regulatory
element
pyroptosis,
which
engaged
kidney
injury.
Meanwhile,
autophagy
involved
renal
inflammation.
Mammalian
target
rapamycin
(mTOR)
plays
an
important
role
pyroptosis
autophagy,
but
its
function
Cd-induced
injury
remains
unclear.
In
this
study,
we
explored
mTOR-mediated
caused
by
Cd
exposure
elucidated
underlying
mechanism.
Our
data
showed
that
reduced
integrity
cell
membranes,
increased
expression
pyroptosis-associated
proteins,
promoted
release
inflammatory
cytokines.
Subsequently,
notable
attenuation
was
observed
following
administration
CY-09,
NLRP3
inhibitor.
addition,
cells.
Importantly,
both
vivo
vitro
experiments,
rapamycin,
mTOR
inhibitor,
downregulated
pyroptosis-related
thereby
significantly
improving
summary,
our
results
indicate
has
significant
protective
effect
on
inflammasome-dependent
induced
exposure,
thus
providing
new
insights
into
prevention
treatment
poisoning.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 24, 2025
Abstract
Targeted
regulation
of
pyroptosis
to
modulate
the
immune
landscape
has
emerged
as
a
novel
design
strategy
for
cancer
immunotherapy
and
anti‐inflammatory
therapy.
However,
acts
double‐edged
sword,
making
it
important
optimize
strategies
functional
materials
appropriately
activate
effective
disease
treatment.
This
paper
summarizes
discusses
structure,
pore
formation,
molecular
mechanisms
“executor”
Gasdermins,
well
events
preceding
following
these
processes.
Subsequently,
focus
is
on
reviewing
that
directly
regulate
Gasdermin
formation
target
those
indirectly
before
after
control
activity.
Finally,
advantages,
disadvantages,
future
prospects
designing
such
are
provided,
aiming
facilitate
precise
design,
pharmacological
investigation,
clinical
translation
pyroptosis‐related
materials.
