Multisubstrate‐based system: a kinetic mechanism study of catechol‐O‐methyltransferase DOI
Fangyuan Wang,

Xianglu Zhou,

Haonan Wang

et al.

FEBS Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

Catechol‐ O ‐methyltransferase (COMT, EC 2.1.1.6 ) can transfer the methyl group from S ‐adenosyl‐ l ‐methionine (SAM) to one of hydroxyl groups a catechol substrate in presence Mg 2+ . However, there is no consensus view kinetic mechanism COMT involving multiple substrates. Further progress requires development methods for determining enzyme behavior and binding mode ligands protein. Here, we establish multisubstrate system covering fluorescence mass spectrometry techniques quantify products COMT‐catalyzed reaction. The substrate, 3‐BTD, be methylated by form single product, 3‐BTMD, with sensitive response conversion SAM ‐homocysteine (SAH) was monitored LC–MS/MS. assays suggested that reaction occurred via an ordered sequential mechanism, which first bound COMT, followed addition 3‐BTD. chemical step involved quaternary complex COMT‐SAM‐Mg ‐3‐BTD, dissociation , SAH. In cooperation molecular dynamics simulation, induced shape change catechol‐binding site, accommodated 3‐BTD facilitated catalysis. These findings provide new insights into contributing previously undescribed functional ligands.

Language: Английский

Flavonoids and Other Polyphenols: Bioactive Molecules from Traditional Medicine Recipes/Medicinal Plants and Their Potential for Phytopharmaceutical and Medical Application DOI Creative Commons
Aekkhaluck Intharuksa, Sompop Kuljarusnont, Yohei Sasaki

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(23), P. 5760 - 5760

Published: Dec. 5, 2024

Currently, natural bioactive ingredients and/or raw materials are of significant interest to scientists around the world. Flavonoids and other polyphenols a major group phytochemicals that have been researched noted as molecules. They offer several pharmacological medical benefits. This current review aims (1) illustrate their benefits for human health, such antioxidant, anti-aging, anti-cancer, anti-inflammatory, anti-microbial, cardioprotective, neuroprotective, UV-protective effects, also (2) perform quality evaluation traditional medicines future application. Consequently, keywords were searched on Scopus, Google Scholar, PubMed so search related publications. Then, those publications carefully checked in order find non-redundant studies matched objective this review. According review, researchers worldwide very interested discovering potential flavonoids polyphenols, used taken from medicinal plants, relation pharmaceutical applications. Many focus health tested using silico, vitro, vivo models. However, few carried out clinical trials trustworthy subject sizes accordance with practice guidelines. Additionally, interesting research directions perspectives highlighted work.

Language: Английский

Citations

5
Network pharmacology and In-depth blood proteomics reveal the mechanism of BQTL capsules in treating bone destruction in osteoarthritis DOI
Haishan Li,

Jiasheng Yang,

Wei Deng

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156867 - 156867

Published: May 1, 2025

Language: Английский

Citations

0
Qualitative and quantitative analysis of major components in Abelmoschus manihot flowers treated with different drying methods using UHPLC Q-Exactive MS and HPLC-PDA DOI

Shaoqing Zhu,

Wei Wang, Xiang Liu

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2024, Volume and Issue: 253, P. 116558 - 116558

Published: Nov. 2, 2024

Language: Английский

Citations

2
Efficacy and Safety of Erzhu Jiedu Decoction Granules in Treating Mid-advanced Hepatitis B Virus-Associated Primary Liver Cancer Patients with Pi (Spleen)-Deficiency and Dampness-Heat Syndrome DOI

Cheng Yang,

Haoyi Wang,

Chengyi Wan

et al.

Chinese Journal of Integrative Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

Language: Английский

Citations

1
Multisubstrate‐based system: a kinetic mechanism study of catechol‐O‐methyltransferase DOI
Fangyuan Wang,

Xianglu Zhou,

Haonan Wang

et al.

FEBS Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

Catechol‐ O ‐methyltransferase (COMT, EC 2.1.1.6 ) can transfer the methyl group from S ‐adenosyl‐ l ‐methionine (SAM) to one of hydroxyl groups a catechol substrate in presence Mg 2+ . However, there is no consensus view kinetic mechanism COMT involving multiple substrates. Further progress requires development methods for determining enzyme behavior and binding mode ligands protein. Here, we establish multisubstrate system covering fluorescence mass spectrometry techniques quantify products COMT‐catalyzed reaction. The substrate, 3‐BTD, be methylated by form single product, 3‐BTMD, with sensitive response conversion SAM ‐homocysteine (SAH) was monitored LC–MS/MS. assays suggested that reaction occurred via an ordered sequential mechanism, which first bound COMT, followed addition 3‐BTD. chemical step involved quaternary complex COMT‐SAM‐Mg ‐3‐BTD, dissociation , SAH. In cooperation molecular dynamics simulation, induced shape change catechol‐binding site, accommodated 3‐BTD facilitated catalysis. These findings provide new insights into contributing previously undescribed functional ligands.

Language: Английский

Citations

0