Frontiers in Microbiology,
Journal Year:
2025,
Volume and Issue:
16
Published: Feb. 3, 2025
Background
Rheumatoid
arthritis
(RA)
is
a
persistent
autoimmune
disorder
marked
by
inflammation
and
joint
damage.
Although
current
treatments,
such
as
disease-modifying
antirheumatic
drugs
(DMARDs),
help
control
symptoms,
they
frequently
cause
substantial
side
effects,
highlighting
the
urgent
need
for
safer
more
effective
alternatives.
Recent
research
indicates
that
gut
microbiota
might
be
pivotal
in
RA
development
through
“gut-joint
axis,”
presenting
novel
therapeutic
possibilities.
Purpose
This
study
seeks
to
explore
potential
of
traditional
Chinese
medicine
(TCM)
compound
new
bitongling
(NBTL)
RA,
with
an
emphasis
on
its
capacity
regulate
suppress
angiogenesis
via
vascular
endothelial
growth
factor
(VEGF)
signaling
pathway.
Methods
We
utilized
collagen-induced
(CIA)
rat
model
assess
impact
NBTL.
The
employed
16S
ribosomal
DNA
(16S
rDNA)
sequencing
analyze
composition,
machine
learning
techniques
identify
characteristic
microbial
taxa,
transcriptomic
analysis
(GSVA)
VEGF
findings
were
further
validated
deep
neural
network
models
vivo
/
vitro
experiments,
including
western
blot,
immunofluorescence,
miRNA
analysis.
Results
NBTL
treatment
markedly
diminished
rats,
evidenced
reduced
expression
TNF-α,
IL-17,
IL-6,
ASC
synovial
tissues.
Histopathological
confirmed
alleviation
Five
f_Mycoplasmataceae
,
s_Metamycoplasma_sualvi
g_Prevotellaceae_Ga6A1_group
identified
associated
NBTL’s
modulation
Gene
set
variation
revealed
significant
downregulation
pathway
following
treatment.
Subsequent
experiments
inhibited
receptors,
VEGFR1
VEGFR2,
along
HIF-1α
(hypoxia-inducible
1-alpha),
thereby
reducing
angiogenesis.
Additionally,
upregulated
miR-20a-5p
miR-223-3p,
contributing
anti-angiogenic
effects.
Conclusion
exhibits
modulating
inhibiting
These
support
use
promising
candidate
treatment,
emphasizing
mechanisms
clinical
application.
Journal of Ethnopharmacology,
Journal Year:
2024,
Volume and Issue:
336, P. 118733 - 118733
Published: Aug. 23, 2024
Smilax
glabra
rhizome
has
a
long
history
been
used
for
clinical
purposes
in
traditional
Chinese
medicinal
treating
various
inflammatory
conditions.
Engeletin
Drug Design Development and Therapy,
Journal Year:
2025,
Volume and Issue:
Volume 19, P. 303 - 324
Published: Jan. 1, 2025
Huanglian-ejiao
decoction
(HED)
is
a
Chinese
traditional
medicinal
formula
evolved
from
the
Shanghan
Lun
(Treatise
on
Febrile
Diseases).
However,
HED
ultimate
mechanism
of
action
remained
indistinct.
Therefore,
this
study
aimed
to
investigate
whether
could
exert
anti-inflammatory
effects
2,4,6-Trinitrobenzenesulfonic
acid
(TNBS)-induced
colitis
(UC)
model
through
regulation
CD4+T
subsets
and
gut
microbiota.
Fifty-eight
major
compounds
in
were
identified
by
UPLC-Q-TOF/MS.
The
therapeutic
efficacy
UC
was
assessed
evaluating
survival
rate
so
on.
Flow
cytometry
employed
assay
percentages
cell.
RT-PCR
Western
blot
took
advantage
detecting
transcription
factors,
inflammatory
tight
junction
proteins.
Transcriptome
sequencing
performed
colon
tissue
16S
rRNA
gene
enforced
intestinal
contents.
administration
enhanced
mice,
significantly
restored
body
weight,
DAI
score,
weight
index,
spleen
index.
effectively
reshaped
barrier
dysfunction,
inhibited
ratio
Th1
Th2
cells,
preserved
Th2/Th1
Tregs/Th17
balance.
Moreover,
notably
decreased
secretion
factors
related
cytokines.
Interestingly,
also
exerts
regulatory
dysbiosis
cumulative
plenteous
beneficial
probiotics
like
Lactobacillus
Bacteroides,
while
inhibiting
overgrowth
opportunistic
pathogens
such
as
Helicobacter.
cell
balance,
well
modulation
microbiota
HED,
provides
further
experimental
evidence
for
feasibility
its
treatment
UC.
Pharmaceuticals,
Journal Year:
2025,
Volume and Issue:
18(1), P. 109 - 109
Published: Jan. 16, 2025
Background:
Cold-dampness
diarrhea
(CDD)
is
a
common
gastrointestinal
disorder
in
children,
characterized
by
and
intestinal
barrier
dysfunction.
Weiling
decoction
(WLD)
frequently
used
clinical
practice
to
treat
CDD,
condition
triggered
multiple
factors.
However,
the
molecular
mechanisms
underlying
its
therapeutic
effects
remain
poorly
understood.
Objectives:
This
study
aimed
evaluate
efficacy
of
WLD
treating
CDD
elucidate
potential
mechanisms.
Methods:
UPLC-HRMS/MS
was
employed
identify
chemical
constituents
absorption
components
plasma
WLD-treated
rats.
Additionally,
rat
model
established
assess
through
comprehensive
approach.
To
these
effects,
network
pharmacology
transcriptomic
analyses
were
performed
signaling
pathways
associated
with
alleviation.
Molecular
docking
flow
cytometry
assays
subsequently
utilized
validate
identified
pathways.
Results:
A
total
223
detected
WLD,
49
rats
UPLC-HRMS/MS.
treatment
significantly
alleviated
symptoms
reduced
damage,
diminished
inflammatory
response.
influenced
key
genes
immune-related
revealed
strong
binding
affinities
between
main
targets
within
Flow
cytometry,
along
analysis
cytokines
transcription
factors,
demonstrated
that
modulated
balance
Th1/Th2
Th17/Treg
cell
populations.
Conclusions:
provides
first
evidence
alleviates
regulating
These
findings
offer
theoretical
basis
for
future
investigations
into
CDD.
BMC Gastroenterology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Jan. 21, 2025
Abstract
Background
and
aim
Ulcerative
colitis
(UC)
is
characterized
by
complex
immunological
interactions
involving
CD4
T
cell
subsets
the
NLRP3
inflammasome,
which
influence
inflammatory
responses.
This
investigation
focused
on
delineating
activation
profiles
of
these
components
their
correlation
with
disease
severity
activity,
assessing
diagnostic
implications
in
UC.
Methods
We
conducted
immunohistochemistry
ELISA
assays
to
measure
markers
expression
inflammasome
UC
patients
versus
controls.
Findings
were
validated
using
analysis,
molecular
docking
ROC
curves.
Results
displayed
increased
Th1
(T-bet,
TNF-α),
Th2
(GATA3,
IL-6),
Th17
(RORγt,
IL-17,
IL-22,
IL-23)
Additionally,
cytokines
(IL-2
IL-4)
significantly
elevated
severe
UC,
while
Treg
(FOXP3,
IL-10,
TGF-β1)
only
mild-to-moderate
Enhanced
activation,
indicated
NLRP3,
Caspase-1,
IL-1β
levels.
These
patterns,
confirmed
through
analysis
docking,
underscored
strong
correlations
among
T-bet,
GATA3,
supporting
proposed
NLRP3/T-bet/GATA3
axis.
axis,
along
other
biomarkers,
showed
associations
severity,
Mayo
score,
UCEIS,
demonstrated
relatively
high
value.
Conclusion
The
axis
provides
a
referable
strategy
for
multi-targeted
combined
treatment
may
serve
as
potential
biomarkers
enhancing
accuracy
guiding
therapy.
Biomarkers,
Journal Year:
2025,
Volume and Issue:
30(1), P. 10 - 22
Published: Jan. 2, 2025
Colorectal
cancer
(CRC)
is
a
substantial
global
health
burden,
with
treatment
outcomes
significantly
influenced
by
the
interaction
between
immune
system
and
tumor
microenvironment.
This
study
aims
to
investigate
role
of
peripheral
blood
cell
subpopulations,
particularly
CD8+
CD28+
T
cells,
in
predicting
response
metastatic
CRC
patients
receiving
bevacizumab
combined
chemotherapy.
A
cohort
45
was
analyzed.
Flow
cytometry
utilized
assess
subpopulations.
Higher
counts
were
associated
better
responses,
including
improved
objective
rates.
In
murine
model,
combination
therapy
inhibited
growth
enhanced
function.
These
findings
highlight
importance
cells
as
potential
biomarkers
for
CRC.
They
also
suggest
that
bevacizumab,
when
chemotherapy,
can
modulate
function
improve
clinical
efficacy.
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2025,
Volume and Issue:
15
Published: March 3, 2025
Inflammatory
Bowel
Disease
(IBD)
is
an
autoimmune
disease
characterized
by
chronic
relapsing
inflammation
of
the
intestinal
tract.
Gut
microbiota
(GM)
and
CD4
+
T
cells
are
important
in
development
IBD.
A
lot
studies
have
shown
that
GM
their
metabolites
like
short-chain
fatty
acids,
bile
acids
tryptophan
can
be
involved
differentiation
through
various
mechanisms,
which
turn
regulate
immune
homeostasis
IBD
patients.
Therefore,
regulating
may
a
potential
therapeutic
direction
for
treatment
Many
Traditional
Chinese
Medicine
(TCM)
formulas
some
herbal
extracts
affect
cell
its
metabolites.
In
this
review,
we
mainly
focus
on
role
correlation
with
We
also
summarize
current
research
progress
regulation
process
TCM.
