Deciphering the Regulatory Potential of Antioxidant and Electron-Shuttling Bioactive Compounds in Oolong Tea DOI Creative Commons

R. Ferrer,

Bor‐Yann Chen, Jon Patrick T. Garcia

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 487 - 487

Published: April 28, 2025

OT has gained attention for its high polyphenol content and therapeutic potential. To elucidate this further, study investigated the electron-shuttling bioactive compounds of evaluated their effect on dysregulated breast cancer (BC) genes. extracts were obtained via solvent extraction (SE) supercritical fluid (SFE), followed by in vitro assays. Phytochemical analysis revealed that ethanol-extracted (OTL-E) had highest polyphenol, flavonoid, tannin contents, correlating with strong antioxidant activity, while water-extracted (OTL-W) exhibited greater bioelectricity-stimulating properties microbial fuel cells (MFC), confirmed cyclic voltammetry (CV). Based phytochemical analyses, SE displayed a better technique isolating compared to SFE. In silico approaches through network pharmacology, molecular docking dynamics simulations polyphenols ortho- or para-dihydroxyl groups targeted BC proteins involved kinase signaling, apoptosis, hormone receptor pathways. Luteolin binding affinities MAPK1 PIK3CA free energy (ΔG) −9.1 −8.4 kcal/mol, respectively. Trajectory-based analyses enthalpy-favored ligand-induced conformational changes these oncoproteins, altering function development. These findings suggest potential as chemopreventive agent, warranting further vivo validation.

Language: Английский

Integrating metabolomics and network pharmacology to explore the mechanism of Xiangshao Sanjie Oral Liquid in treating rats with mammary gland hyperplasia DOI
Yu Zhang, Junmin Li, Dan Peng

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: 343, P. 119493 - 119493

Published: Feb. 15, 2025

Language: Английский

Citations

0
Deciphering the Regulatory Potential of Antioxidant and Electron-Shuttling Bioactive Compounds in Oolong Tea DOI Creative Commons

R. Ferrer,

Bor‐Yann Chen, Jon Patrick T. Garcia

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 487 - 487

Published: April 28, 2025

OT has gained attention for its high polyphenol content and therapeutic potential. To elucidate this further, study investigated the electron-shuttling bioactive compounds of evaluated their effect on dysregulated breast cancer (BC) genes. extracts were obtained via solvent extraction (SE) supercritical fluid (SFE), followed by in vitro assays. Phytochemical analysis revealed that ethanol-extracted (OTL-E) had highest polyphenol, flavonoid, tannin contents, correlating with strong antioxidant activity, while water-extracted (OTL-W) exhibited greater bioelectricity-stimulating properties microbial fuel cells (MFC), confirmed cyclic voltammetry (CV). Based phytochemical analyses, SE displayed a better technique isolating compared to SFE. In silico approaches through network pharmacology, molecular docking dynamics simulations polyphenols ortho- or para-dihydroxyl groups targeted BC proteins involved kinase signaling, apoptosis, hormone receptor pathways. Luteolin binding affinities MAPK1 PIK3CA free energy (ΔG) −9.1 −8.4 kcal/mol, respectively. Trajectory-based analyses enthalpy-favored ligand-induced conformational changes these oncoproteins, altering function development. These findings suggest potential as chemopreventive agent, warranting further vivo validation.

Language: Английский

Citations

0