Aconiti Lateralis Radix Praeparata ameliorates heart failure via PI3K/AKT/Bnip3 pathway

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

Background Chronic heart failure (CHF) is one of the leading causes high mortality worldwide. It characterized by pathological hypertrophy and poses a major threat to human health. Aconiti Lateralis Radix Praeparata widely used in ancient China treat CHF. However, pathology obscured, necessitating further exploration. Methods Prospective targets were predicted network analysis. A transverse aortic constriction (TAC) mice model was subsequently constructed determine effects aqueous extract (AEA) on The echocardiography performed investigate cardiac function. Histopathological analysis tissue conducted assess myocardial fibrosis. Nontargeted metabolomics analyze serum metabolites. phosphorylation level PI3K AKT, downstream such as Bnip3, p62, Atg5, LC3II measured Western blotting. In vitro , norepinephrine (NE) stimulate hypertrophy. Parameters reactive oxygen species levels, mitochondrial membrane potential, ATP concentration, CK/MB content detected H9c2 cells. Results AEA significantly alleviated Network indicated participation AKT CHF, modulated Praeparata. vivo administration effectively ameliorated performance, evidenced elevation ejection fraction. displayed diminishment collagen fiber. Metabolomics showed that several metabolites tetrahydroxycorticosterone, decylubiquinone taurocholic acid increased TAC serum. Additionally, levels expression Drp1, Opa1, Atg5 altered group. NE stimulation cell surface area deteriorated functions partially reversed results, mechanism associated with mitophagy. Conclusion This study revealed improved function via PI3K/AKT/Bnip3 pathway.

Language: Английский

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The salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 3, 2025

Saliva, which is a critical component of the oral ecosystem, undergoes dynamic changes, particularly during onset and progression periodontitis. This study used Gas Chromatography-Mass Spectrometry (GC-MS), reliable high-throughput tool for metabolomic analysis, to detect salivary metabolic shifts across various stages periodontitis (T1-T4). We compared differential changes in metabolites between HC T1 groups, T2 T3 T4 groups. By analysing saliva samples from 116 individuals-10 healthy controls (HC) 106 patients with (22 individuals) (28 individuals), we identified including Glucose, 3-Aminobutanoic Acid, N-(1-Cyclopropylethyl) Aniline, Methylmalonic Acid. Compared HC, exhibited progressive concentration correlating severity disease. Furthermore, KEGG pathway analysis was elucidate pathways involved development Our findings demonstrate potential as biomarkers monitoring offer valuable insights into its pathogenesis therapeutic targets.

Language: Английский

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Affinity-purified targets screening facilitates active components discovery of Chinese formula —HuGan Tablets as a case

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119703 - 119703

Published: April 1, 2025

Language: Английский

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Changdiqing Decoction (CDQD) Ameliorates Colitis via Suppressing Inflammatory Macrophage Activation and Modulating Gut Microbiota

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156856 - 156856

Published: May 1, 2025

Language: Английский

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Kehuang capsule inhibits MAPK and AKT signaling pathways to mitigate CCl4-induced acute liver injury

Liver Research, Journal Year: 2024, Volume and Issue: 8(4), P. 269 - 281

Published: Nov. 30, 2024

Language: Английский

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