Targeting neutrophil dysfunction in acute lung injury: insights from active components of Chinese medicine

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156664 - 156664

Published: March 1, 2025

Language: Английский

---

Boeravinone C ameliorates lipid accumulation and inflammation in diabetic kidney disease by activating PPARα signaling

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119398 - 119398

Published: Jan. 1, 2025

Language: Английский

---

Inhibitory effects of cornuside on human liver cytochrome P450 enzymes

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Language: Английский

---

MiR-203 improved renal cell injury in diabetic nephropathy by targeting SOCS6/SOCS7 and inhibiting JAK/STAT pathway activation

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 28, 2025

This study investigates the role of miR-203 in regulating renal cell injury diabetic nephropathy by targeting suppressor cytokine signaling (SOCS) proteins SOCS6 and SOCS7. Using NRK cells, we assessed apoptosis through flow cytometry TUNEL assays, while real-time quantitative PCR (RT-PCR) quantified miRNA mRNA expressions. Cell viability was measured using CCK-8 assay, levels were determined ELISA. We also evaluated reactive oxygen species (ROS) malondialdehyde (MDA) with specific assay kits. The dual luciferase confirmed interaction Western blotting analyzed protein key molecules including JAK1, p-JAK1, JAK2, p-JAK2, STAT3, p-STAT3.Our findings revealed that high glucose (HG) treatment reduced levels, leading to decreased proliferation, increased concentrations (TNF-α, IL-1β, IL-4, IL-6), heightened ROS MDA apoptosis. Notably, mimics counteracted HG's detrimental effects, inhibitors exacerbated them. Mechanistically, directly SOCS7 expression, thereby inhibiting JAK/STAT3 signaling. Thus, provides protective effects against modulating SOCS their associated pathways.

Language: Английский

---

Single-nucleus RNA sequencing reveals ARHGAP28 expression of podocytes as a biomarker in human diabetic nephropathy

Open Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

Abstract Introduction Diabetic kidney disease (DKD) represents serious diabetes-associated complications, and podocyte loss is an important histologic sign of DKD. The cellular molecular profiles podocytes in DKD have yet to be fully elucidated. Methods This study analyzed kidney-related single-nucleus RNA-seq datasets (GSE131882, GSE121862, GSE141115) human diabetic glomeruli transcriptome profiling (GSE30122). ARHGAP28 expression was validated by western blot immunohistochemistry. Results In tissues, 154 differentially expressed genes (DEGs) were identified podocytes, which enriched biological processes related nephron development extracellular matrix–receptor interactions. Similarly, the mouse kidney, 344 DEGs found, clustering pathways associated with renal signaling mechanisms like PI3K/Akt (phosphatidylinositol-3 kinase/protein kinase B) PPAR (peroxisome proliferator-activated receptor). glomeruli, 438 identified, showing significant enrichment nephropathy. Venn analysis revealed 22 common across being notably overexpressed podocytes. nephropathy model using db/db mice showed that significantly upregulated cortex glomeruli. vitro studies a high-glucose corroborated these findings. Conclusions Collectively, this provides insight into function diagnosis indicates potential biomarker

Language: Английский

---

Supplemented Gegen Qinlian Decoction Formula attenuates podocyte mitochondrial fission and renal fibrosis in diabetic kidney disease by inhibiting TNF-α-mediated necroptosis, compared with empagliflozin

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 334, P. 118572 - 118572

Published: July 16, 2024

Language: Английский

---