Integrated bioinformatics and multi-omics to investigate the mechanism of Rhododendron molle Flos-induced hepatotoxicity

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: 341, P. 119308 - 119308

Published: Jan. 1, 2025

Language: Английский

Adaptive Defense Mechanism During Flowering Period of Rhododendron decorum Revealed by Comparative Transcriptomic Analysis

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 559 - 559

Published: Feb. 12, 2025

Rhododendron decorum, a widely distributed species in southwestern China, is recognized not only for its significant ornamental value but also as culinary resource local tribes. However, the defense mechanisms underlying ecological adaptations of R. decorum remain to be elucidated. In this study, we conducted comparative transcriptomic analyses various organs (corolla, androecium/gynoecium and leaves) collected from two distinct regions. Approximately 186.98 Gb clean data were generated three across these Through de novo assembly, total 92,025 unigenes obtained nearly half them (43,515 unigenes) successfully annotated. Enrichment analysis differentially expressed genes (DEGs) within groups different (HQI/LFI, HQO/LFO HQL/LFL, respectively) revealed that distribution Heqing region exhibited an increased requirement plant immunity, including resistance diseases, insects, herbivores organs. Conversely, Lijiang showed greater reliance on environmental factors, such cold tolerance, aromatic compounds production, attraction pollinating insects. Notably, validation 21 pivotal identified significantly regulated enrichment pathways functional consistency KEGG among The disparities observed transcriptome regions provide valuable insight into understanding adaptive mechanism.

Language: Английский

Exploring the Bioactive Constituents and Therapeutic Mechanisms of Sanhuang Xiexin Decoction in Hypercholesterolemia Treatment Using UHPLC‐Q‐Orbitrap‐MS/MS Integrated With Metabolomics and Network Pharmacology

Rapid Communications in Mass Spectrometry, Journal Year: 2025, Volume and Issue: 39(15)

Published: May 4, 2025

ABSTRACT Rational Sanhuang Xiexin Decoction (SHD), a traditional Chinese medicine, has been used widely in East Asian Countries for hundreds of years and recent studies have implied its lipid‐lowering cardiovascular protective effects. However, the active components potential mechanism SHD treating hypercholesterolemia (HC) remain unclear. Methods UHPLC‐Q‐Orbitrap‐MS/MS was to annotate SHD, then, efficacy alleviating HC demonstrated vitro vivo . Subsequently, metabolomics by employed identify discriminative metabolites metabolic pathways involved against HC. Network pharmacology applied explore bioactive components. Then, integrated approach proposed elucidate possible targets mechanisms. Finally, molecular docking operated validate Results A total 180 chemical were identified 18 prototype confirmed Metabolomics study revealed 12 metabolites, five associated with SHD. analysis further compounds 107 targets. suggested that exerts effects modulating purine glycerophospholipid metabolism through regulation key targets, including phosphodiesterase 5 (PDE5), acetylcholinesterase (ACHE), phospholipase A2 group VII (PLA2G7), xanthine dehydrogenase (XDH). Conclusion therapeutic on multiple This lays groundwork clinical application treatment paves way exploration underlying

Language: Английский