Mechanism of Anchang Decoction in Treatment of Anti-Inflammatory Effect Based on Network Pharmacology, Molecular Docking and Experimental Verification DOI Creative Commons
Wei Tian,

Weiqian Zhou,

Shi Huang

et al.

Natural Product Communications, Journal Year: 2024, Volume and Issue: 19(9)

Published: Sept. 1, 2024

Context A prescription medication called Anchang Decoction (ACD) has anti-inflammatory properties. Objective To evaluate the mechanism of ACD against inflammation. Materials and methods The “pharmacodynamic constituents - potential targets” “protein interaction networks” were mapped using Cytoscape software STRING database, respectively. degree binding between important pharmacodynamic components possible targets was then examined molecular docking analysis Autodock Vina PyMOL software, GO KEGG pathway enrichment David database Weishengxin online tool. These findings eventually confirmed in vitro. Results After intersection two, 539 inflammatory 217 related targets, 34 main active components, 42 obtained. include AKT1, MAPK14, SRC, EGFR, GSK3B, MMP9, MMP2, PTGS2, SYK, ESR1, MMP2 results. three key are chosen as downstream validation for experimental verification. Furthermore, colonic tissue mucosa group undamaged comparison to model group, there no sign cell infiltration. According CCK-8 data, treatment with 20% drug-containing serum resulted a significant increase RAW264.7 viability (P < 0.05) when compared normal group; Serum ACD-containing may considerably lower NO content macrophage inflammation prevent production markers like TNF-α IL-6 0.05); expression levels SRC proteins reduced by inflammation, according Western Blot data 0.05). Discussion conclusions exerts effects through multi-component target, involve inhibition release cytokines AKT, MAPK non-receptor tyrosine kinase signaling pathways. Here, partially clarified experimentally validated, offering theoretical evidence more effective clinical application.

Language: Английский

An Integrated Strategy for Deciphering the Action Mechanism of Emplastrum: Prescription Analysis- Component Identification- Virtual Screening and Affinity Testing in the Case of Yaoshen Gao DOI
Zeny Feng, Han Chen, Na Zhang

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119369 - 119369

Published: Jan. 1, 2025

Language: Английский

Citations

1
Substitutability evaluation and resource utilization of non‐medicinal parts of Ardisia crenata Sims, a traditional ethnic medicine DOI

Jie Peng,

Sha Liu,

Fusong Liu

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(1)

Published: Jan. 1, 2025

Abstract The severe scarcity of wild resources Ardisia crenata Sims (AS) has greatly limited its current clinical and pharmaceutical applications. We aimed to demonstrate the substitutability stems leaves for AS roots. In this study, 28 local samples from Guizhou Province China were selected a comprehensive comparison quality markers, chemical composition efficacy. HPLC analysis showed that both contained bergenin, main active ingredient AS. Metabolomics studies three parts was 99% similar. Network pharmacology similar signalling targets including STAT3 MAPK1, AKT1 EP300, SRC TP53, targeted by parts. Animal experiments further demonstrated extracts different plant relieved pathological symptoms acute pharyngitis, decreased serum inflammatory factors, showing sum, these findings strongly proved can be used as supplements or substitutes roots, providing powerful strategy solve problem resource shortage promoting optimal utilization study also anticipated some additional benefits, expansion natural sources bergenin discovery potential cancer‐related

Language: Английский

Citations

0
The role and application of bioinformatics techniques and tools in drug discovery DOI Creative Commons
Shujun Zhang, Kaijie Liu, Yafeng Liu

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 13, 2025

The process of drug discovery and development is both lengthy intricate, demanding a substantial investment time financial resources. Bioinformatics techniques tools can not only accelerate the identification targets screening refinement candidates, but also facilitate characterization side effects prediction resistance. High-throughput data from genomics, transcriptomics, proteomics, metabolomics make significant contributions to mechanics-based reuse. This paper summarizes bioinformatics technologies in research their roles applications development, aiming provide references for new drugs realization precision medicine.

Language: Английский

Citations

0
Molecular insight of nanosized Ba-Hao herbal ointment in accelerating chronic wound healing DOI Creative Commons
Hui Qian,

Lu Chen,

Xiaoman Zhang

et al.

Nanoscale Advances, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Chronic wound treatment poses a substantial challenge to healthcare systems. Certain herbal medicines have demonstrated clinical efficacy in promoting chronic healing, yet their therapeutic mechanisms at the molecular level remain elusive due complex composition and multifaceted nature. In this study, Ba-Hao ointment (BHO), sophisticated formulation with diverse ingredients, is selected as model precisely investigate its wound-healing mechanism. Network pharmacology analysis docking simulations reveal that BHO specifically interacts key proteins, including vascular endothelial growth factor A (VEGFA), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), suggesting ability modulate critical biological pathways involved inflammation tissue regeneration. Experimental validation further demonstrates significantly promotes cell proliferation, suppresses bacterial infection, enhances expression of essential factors such epidermal (EGF) VEGFA normal human dermal fibroblasts (NHDF), all which are vital for effective healing. vivo studies confirm accelerates closure, reduces inflammation, development well-organized granulation via activation PI3K-Akt signaling pathway. This study interesting since it unveils BHO's role furthering medicine insights.

Language: Английский

Citations

0
Selenoprotein S (SELENOS) is a potential prognostic biomarker for Brain lower grade glioma DOI

Yuetong Wang,

Kai Qu,

Zengrun Xia

et al.

Journal of Trace Elements in Medicine and Biology, Journal Year: 2024, Volume and Issue: 86, P. 127539 - 127539

Published: Oct. 4, 2024

Language: Английский

Citations

1
Mechanism of Anchang Decoction in Treatment of Anti-Inflammatory Effect Based on Network Pharmacology, Molecular Docking and Experimental Verification DOI Creative Commons
Wei Tian,

Weiqian Zhou,

Shi Huang

et al.

Natural Product Communications, Journal Year: 2024, Volume and Issue: 19(9)

Published: Sept. 1, 2024

Context A prescription medication called Anchang Decoction (ACD) has anti-inflammatory properties. Objective To evaluate the mechanism of ACD against inflammation. Materials and methods The “pharmacodynamic constituents - potential targets” “protein interaction networks” were mapped using Cytoscape software STRING database, respectively. degree binding between important pharmacodynamic components possible targets was then examined molecular docking analysis Autodock Vina PyMOL software, GO KEGG pathway enrichment David database Weishengxin online tool. These findings eventually confirmed in vitro. Results After intersection two, 539 inflammatory 217 related targets, 34 main active components, 42 obtained. include AKT1, MAPK14, SRC, EGFR, GSK3B, MMP9, MMP2, PTGS2, SYK, ESR1, MMP2 results. three key are chosen as downstream validation for experimental verification. Furthermore, colonic tissue mucosa group undamaged comparison to model group, there no sign cell infiltration. According CCK-8 data, treatment with 20% drug-containing serum resulted a significant increase RAW264.7 viability (P < 0.05) when compared normal group; Serum ACD-containing may considerably lower NO content macrophage inflammation prevent production markers like TNF-α IL-6 0.05); expression levels SRC proteins reduced by inflammation, according Western Blot data 0.05). Discussion conclusions exerts effects through multi-component target, involve inhibition release cytokines AKT, MAPK non-receptor tyrosine kinase signaling pathways. Here, partially clarified experimentally validated, offering theoretical evidence more effective clinical application.

Language: Английский

Citations

0