Quality Study of HuangqiGuizhiWuwu Decoction Based on Ultra Performance Liquid Chromatography Fingerprinting, Chemical Pattern Recognition and Multi‐Component Quantitative Analysis DOI Open Access
Xin‐Yue Hu,

Qi‐Song Chen,

Shun‐Qing He

et al.

Separation Science Plus, Journal Year: 2025, Volume and Issue: 8(3)

Published: Feb. 28, 2025

ABSTRACT HuangqiGuizhiWuwu decoction (HGWD) possesses a complex and diverse chemical composition, rendering the identification of its components challenging. In this study, UPLC fingerprinting profile HGWD was elucidated method determining multiple in proposed. Common peaks were selected from 12 batches HGWD, Chinese herbal chromatographic fingerprint similarity evaluation system (2012) employed for analyses. The corresponding to common identified using UPLC‐Q‐TOF‐MS. Chemical analyses conducted evaluate quality different identify primary components. similarities profiles all > 0.98, with 15 confirmed divided into three categories, OPLS‐DA used cinnamic acid cinnamaldehyde as characteristic that caused differences. five which included albiflorin, paeoniflorin, calycosin‐7‐O‐β‐D‐glucoside, acid, cinnamaldehyde, exhibited good linear relationships average recovery rates relative standard deviations. This study established proposed simple, feasible, sensitive, reproducible content determination method. Moreover, our findings provide robust foundation standardization HGWD.

Language: Английский

Quality Study of HuangqiGuizhiWuwu Decoction Based on Ultra Performance Liquid Chromatography Fingerprinting, Chemical Pattern Recognition and Multi‐Component Quantitative Analysis DOI Open Access
Xin‐Yue Hu,

Qi‐Song Chen,

Shun‐Qing He

et al.

Separation Science Plus, Journal Year: 2025, Volume and Issue: 8(3)

Published: Feb. 28, 2025

ABSTRACT HuangqiGuizhiWuwu decoction (HGWD) possesses a complex and diverse chemical composition, rendering the identification of its components challenging. In this study, UPLC fingerprinting profile HGWD was elucidated method determining multiple in proposed. Common peaks were selected from 12 batches HGWD, Chinese herbal chromatographic fingerprint similarity evaluation system (2012) employed for analyses. The corresponding to common identified using UPLC‐Q‐TOF‐MS. Chemical analyses conducted evaluate quality different identify primary components. similarities profiles all > 0.98, with 15 confirmed divided into three categories, OPLS‐DA used cinnamic acid cinnamaldehyde as characteristic that caused differences. five which included albiflorin, paeoniflorin, calycosin‐7‐O‐β‐D‐glucoside, acid, cinnamaldehyde, exhibited good linear relationships average recovery rates relative standard deviations. This study established proposed simple, feasible, sensitive, reproducible content determination method. Moreover, our findings provide robust foundation standardization HGWD.

Language: Английский

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