Ultrasonic-assisted extraction of flavonoids from Amomum villosum Lour. Using natural deep eutectic solvent: Process optimization, comparison, identification, and bioactivity

Ultrasonics Sonochemistry, Journal Year: 2025, Volume and Issue: 116, P. 107304 - 107304

Published: March 12, 2025

Amomum villosum Lour. (A. villosum) is widely utilized in culinary and medicinal contexts due to its rich diversity of bioactive compounds. In this study, an ultrasonic-assisted extraction (UAE) method using a natural deep eutectic solvent (NaDES) was employed extract flavonoids from A. villosum. The NaDES composed betaine glycerol (Bet-Gly) exhibited effective performance as solvent, evidenced by high efficiency. Optimal conditions (water content: 30 %, solid-liquid ratio: 1:20 g/mL, temperature: 70 °C, ultrasonic power: 85 % P, time: mins) were established via single-factor experiments. maximum total flavonoid content (TFC) 82.22 ± 0.39 mg rutin equivalent per gram dry weight (mg Rut/g DW) achieved under optimized conditions. Comparative studies revealed that the UAE-NaDES afforded higher TFC DPPH radical scavenging activity values, broader diversity, demonstrated better capacity stabilize them compared conventional solutions. Furthermore, favorable biocompatibility properties, resulting displayed remarkable anti-inflammatory anti-cancer effects. These findings demonstrate efficient for villosum, with potential applications food pharmaceutical industries, thereby minimizing removal steps.

Language: Английский

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Traditional usages, chemical metabolites, pharmacological activities, and pharmacokinetics of Boesenbergia rotunda (L.) Mansf.: a comprehensive review

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

Boesenbergia rotunda : (L.) Mansf. (family Zingiberaceae), also known as fingerroot, is a medicinal and food plant that widely distributed in southern China, Southeast Asia, South Asia. It traditional herb spice for its beneficial effects on Qi , appetite, stagnation pain relief. The objective of this study to conduct comprehensive systematic review the botanical characteristics, applications, phytochemical metabolites, pharmacological properties, toxicology, quality control measures, pharmacokinetics, clinical applications B. . A bibliometric analysis current studies was conducted facilitate further exploration utilization functional pharmaceutical industries. These data were collected from PubMed, Web Science, Google Scholar, China National Knowledge Infrastructure doctoral master’s theses other books scientific databases by searching keywords Phytochemical has revealed presence flavonoids, monoterpenes, alkaloids, aromatic phenols, metabolites exhibiting wide range biological activities such anti-cancer, nephroprotective, anti-inflammatory, anti-bacterial, hepatoprotective, anti-obesity, anti-oxidant effects, both vivo vitro In paper, research discussed depth combining application modern research, aiming provide valuable reference future practical

Language: Английский

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Separation and Identification of Non-Volatile Sour and Bitter Substances in Amomum villosum L. by Ultra-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry and Electronic Tongue Analysis, as Well as Their In Vitro Anti-Tumor Activity

Separations, Journal Year: 2025, Volume and Issue: 12(4), P. 77 - 77

Published: March 28, 2025

Amomum villosum L. is a perennial herbaceous belonging to the ginger family. Due its unique aroma, it widely used in alcoholic beverages and food processing. Unfortunately, issues with bitterness sourness occur, which affect taste quality of processed products. In this study, non-volatile sour bitter substances were systematically isolated, purified, characterized through combination chromatographic separation techniques ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS). The results indicate that three compounds (DL-malic acid, protocatechuic p-hydroxybenzoic acid) one compound (catechin) identified for first time vitro anti-tumor activity was screened determined using Cell Counting Kit-8 (CCK-8) assays, 5-Ethynyl-2′-deoxyuridine (EdU) staining experiment, scratch assays. reveal substance catechin (25–100 μg/mL) exhibited significant inhibitory effects, inhibited proliferation migration human non-small cell lung cancer A549 cells dose-dependent mechanisms. This investigation also reveals influence different traditional extraction solvents on degree extracts, providing theoretical basis improving pharmacological utilization extracts.

Language: Английский

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Cloning, Expression, and Bioinformatics Analysis of the AvFD1 Gene in Amomum villosum Lour

Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 457 - 457

Published: April 24, 2025

As a perennial medicinal plant in the Zingiber genus, Amomum villosum Lour. faces agricultural challenges due to its prolonged vegetative and reproductive growth phases, which hinder efficient pollination delay fruiting. To address this limitation, present study aimed identify FD gene involved regulating flowering A. provide basis for research on molecular mechanisms of early fruiting cultivars. Based differentially expressed AvFD1 obtained from transcriptome database plants controls, specific primers were designed PCR clone full-length sequence AvFD1. The characteristics cloned analyzed using online bioinformatics software. expression profiles various tissues 1- 2-year bearing varieties investigated by quantitative real-time PCR. This successfully FD1 villosum, marking first reported characterization species. Tissue-specific analysis revealed significantly elevated levels stolon tips flower buds compared tender leaves, suggesting potential role as positive regulator initiation. establishes essential data subsequent functional validation villosum.

Language: Английский

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Preparation of Ovalbumin/Xanthan Gum/Chitosan Pickering Emulsion Oleogel Added with Amomum villosum Lour. Extract and Its Application in Cookies

Gels, Journal Year: 2024, Volume and Issue: 10(11), P. 683 - 683

Published: Oct. 23, 2024

In this study, a new oleogel system was constructed and used as fat substitute in the processing of cookies. The preparation process

Language: Английский

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Amomum villosum Lour. alleviates pre-eclampsia by inducing enrichment of Bifidobacterium bifidum through vanillic acid to inhibit placental ferroptosis

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 119217 - 119217

Published: Dec. 1, 2024

Amomum villosum Lour. (AVL), a traditional Chinese medicine, is widely used to pregnancy-related vomiting and prevent miscarriage. Pre-eclampsia (PE) severe pregnancy syndrome. Recent studies have demonstrated interactions between PE the digestive system. However, it uncertain that AVL against was associated with gut.

Language: Английский

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