Electroacupuncture combined with trigonelline inhibits pyroptosis in cerebral ischemia-reperfusion by suppressing autophagy via the PI3K/AKT/mTOR signaling pathway

Brain Research Bulletin, Journal Year: 2025, Volume and Issue: unknown, P. 111200 - 111200

Published: Jan. 1, 2025

Electroacupuncture (EA) and trigonelline (TG) have been reported to be beneficial in alleviating cerebral ischemia/reperfusion injury (CIRI). However, the synergistic effects of EA TG CIRI underlying mechanism not demonstrated. Rats were subjected middle artery occlusion (MCAO) surgery reperfusion (MCAO/R) establish a model. Neurological deficit score was evaluated using Garcia's scale. Cerebral infarction rats determined TTC staining. Brain tissue morphology assessed by HE The expression various proteins measured IF assay western blot. or treatment could effectively ameliorate neurological disorders, attenuate reduce neuronal damage brain rats. In addition, suppressed autophagy pyroptosis More importantly, intervention observed ameliorating suppressing pyroptosis, while Rapa, an inducer autophagy, strengthened these MCAO/R-induced Furthermore. Rapa reversed combination with TG-mediated improvement suppression Notably, PI3K/AKT/mTOR pathway inactivated combined enhanced activation pathway. LY294002, inhibitor pathway, stimulated pyroptosis. which dependent on inhibition through signaling

Language: Английский

Luteolin alleviates olfactory dysfunction in eosinophilic chronic rhinosinusitis through modulation of the TLR4/NF-κB signaling pathway

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 148, P. 114189 - 114189

Published: Feb. 1, 2025

Language: Английский

Insight of action mechanism of Astragaloside IV for relieving of cerebral ischemic injury in a rat model of middle cerebral artery occlusion reperfusion via proteomics and network pharmacology

Journal of Natural Medicines, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Language: Английский